Diagnosis and discrimination of autoimmune Graves' disease and Hashimoto's disease using thyroid-stimulating hormone receptor-containing recombinant proteoliposomes

Fukushima, Hidetaka; Matsuo, Hideaki; Imamura, Koji; Morino, Kazuhide; Okumura, Katsuhiko; Tsumoto, K.; Yoshimura, Teizo

doi:10.1016/j.jbiosc.2009.06.006

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…Notably, AABs to cytokine and chemokine antigens (IL6 and CSF2) involved in immune defense, together with lung specific proteins (gastrin release peptide (GRP) and serpin family B member 3 (SERPINB3), were predominantly elevated in asymptomatic females. By contrast, thyroid stimulating hormone receptor (TSHR) and lysine demethylase 6B (KDM6B), which are known primary antigens in autoimmune diseases, including Graves' disease and Hashimoto's disease [ 13 ] and systemic sclerosis (SS also termed scleroderma) [ 14 ] were highly expressed in asymptomatic males. Among all participants who had at least mild symptoms, the range and degree of AAB reactivity was more pronounced in males compared to females (Fig.…”

Section: Resultsmentioning

confidence: 99%

Paradoxical sex-specific patterns of autoantibody response to SARS-CoV-2 infection

et al. 2021

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Background Pronounced sex differences in the susceptibility and response to SARS-CoV-2 infection remain poorly understood. Emerging evidence has highlighted the potential importance of autoimmune activation in modulating the acute response and recovery trajectories following SARS-CoV-2 exposure. Given that immune-inflammatory activity can be sex-biased in the setting of severe COVID-19 illness, the aim of the study was to examine sex-specific autoimmune reactivity to SARS-CoV-2 in the absence of extreme clinical disease. Methods In this study, we assessed autoantibody (AAB) reactivity to 91 autoantigens previously linked to a range of classic autoimmune diseases in a cohort of 177 participants (65% women, 35% men, mean age of 35) with confirmed evidence of prior SARS-CoV-2 infection based on presence of antibody to the nucleocapsid protein of SARS-CoV-2. Data were compared to 53 pre-pandemic healthy controls (49% women, 51% men). For each participant, socio-demographic data, serological analyses, SARS-CoV-2 infection status and COVID-19 related symptoms were collected by an electronic survey of questions. The symptoms burden score was constructed based on the total number of reported symptoms (N = 21) experienced within 6 months prior to the blood draw, wherein a greater number of symptoms corresponded to a higher score and assigned as more severe burden. Results In multivariable analyses, we observed sex-specific patterns of autoreactivity associated with the presence or absence (as well as timing and clustering of symptoms) associated with prior COVID-19 illness. Whereas the overall AAB response was more prominent in women following asymptomatic infection, the breadth and extent of AAB reactivity was more prominent in men following at least mildly symptomatic infection. Notably, the observed reactivity included distinct antigens with molecular homology with SARS-CoV-2. Conclusion Our results reveal that prior SARS-CoV-2 infection, even in the absence of severe clinical disease, can lead to a broad AAB response that exhibits sex-specific patterns of prevalence and antigen selectivity. Further understanding of the nature of triggered AAB activation among men and women exposed to SARS-CoV-2 will be essential for developing effective interventions against immune-mediated sequelae of COVID-19.

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Section: Resultsmentioning

confidence: 99%

Paradoxical sex-specific patterns of autoantibody response to SARS-CoV-2 infection

et al. 2021

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“…BV particles were collected with a conventional protocol as previously described. [28][29][30][31][32][33][34] BVs were precipitated from the supernatant using a Beckman Coulter L-70 ultracentrifuge (107,000 × g, 30 min, 15°C). The pellet was completely resuspended in~3-4 mL of phosphate-buffered saline (PBS; 1 mM Na 2 HPO 4 , 10.5 mM KH 2 PO 4 , 140 mM NaCl, 40 mM KCl; pH 6.2 adjusted with NaOH aq.).…”

Section: Methodsmentioning

confidence: 99%

“…23) The utility of BVs as gene carriers to mammals was reported, 24,25) and experimental kits are commercially available. 26) When fused with liposomes, protein-expressing BV particles can be used to prepare recombinant proteoliposomes by activating the endogenic fusion protein GP64 27) under acidic conditions, as reported by Yoshimura et al [28][29][30][31][32][33][34] As mentioned above, BV particles have become attractive as nanomaterials, similar to other virus particles. AcNPV BVs are recovered from the culture supernatant of BV-infected host cells (Sf9, Sf21, etc.…”

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confidence: 94%

The method used to culture host cells (Sf9 cells) can affect the qualities of baculovirus budding particles expressing recombinant proteins

Hattori

Nakanishi

Mori

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Budded virus (BV) particles of baculovirus (Autographa californica nucleopolyhedrovirus, AcNPV) are harvested from the supernatant of liquid culture of Sf9 host cells by ultracentrifugation. Using polyacrylamide gel electrophoresis, Western blot and transmission electron microscopy (TEM) of BV samples fractionated closely by sucrose density gradient centrifugation, we observed that BVs exhibited different qualities depending on whether they had been harvested from the supernatant from a standing (static), shaking (suspension), or standing/shaking (pre-/post-infection) culture of Sf9 cells. The amount of BV protein apparently increased in the order of standing, standing/shaking, and shaking procedure, and the yield of intact particles showed an opposite trend. TEM observation clearly showed that appropriate fractions of the standing and standing/shaking cultures contained more intact BV particles than those from the shaking culture. These results suggest that the qualities of recombinant BV particles may be related to the culture conditions of the host cells.

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“…In Graves's diseases, an autoimmune disease of the thyroid gland, antibodies are produced against the thyroid protein thyroglobulin. These antibodies are called Thyroid Stimulating Hormones Receptors (TSHR) antibodies results in the increase in thyroid synthesis and section and thyroid growth as well as all accompanying symptoms [15][16][17]. In some autoimmune blood disorders, antibodies are produced against the body red and white blood cells, while in other autoimmune disorders, antibodies attack a wide range of tissues and organs resulting in more debilitating symptoms [18].…”

Section: Introductionmentioning

confidence: 99%

Probiotics Applications in Autoimmune Diseases

Al‐Salami¹,

Caccetta²,

Goločorbin-Kon³

et al. 2012

Probiotics

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Diagnosis and discrimination of autoimmune Graves' disease and Hashimoto's disease using thyroid-stimulating hormone receptor-containing recombinant proteoliposomes

Cited by 14 publications

References 23 publications

Paradoxical sex-specific patterns of autoantibody response to SARS-CoV-2 infection

Paradoxical sex-specific patterns of autoantibody response to SARS-CoV-2 infection

The method used to culture host cells (Sf9 cells) can affect the qualities of baculovirus budding particles expressing recombinant proteins

Probiotics Applications in Autoimmune Diseases

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