Diagnosis and grading of malignancy in squamous epithelial lesions of the larynx with DNA cytophotometry

Abstract

For diagnostic purposes 14 histologically benign, 15 dysplastic, and 16 malignant squamous epithelial lesions were subjected to DNA cytophotometry. Results were computed according to an algorithm for DNA diagnosis and grading of malignancy. In cases of unequivocally malignant or benign lesions all DNA diagnoses agreed with the histologic diagnoses. In one case the allegedly faulty DNA diagnosis of cancer was proven to be correct on clinical follow-up. Within the group of histologically mild to moderate dysplasias the algorithm identified four cases as malignant that were proved to be malignant either in the follow-up or at another site of the same lesion. With the aid of the DNA malignancy grade two groups of patients with squamous epithelial carcinomas of the larynx could be discerned with a highly significant difference according to their survival times.

Morphoquantitative Analysis of the Intraepithelial Neoplasic Uterine Cells by Computer Image System

“…Our studies reveal a different concentrations in DNA cells distribution, as to moderate dysplasia (CIN II) indication and morphologic diagnostic, in opposition with those with dysplasia in benign level (CIN I), leading to a possible observation of the beginning of a lesion as observed by Böcking et al (1984Böcking et al ( , 1985. According to Saccomano (1982), lesions considered moderate dysplasias (CIN II) change to carcinoma in situ within eight years.…”

“…Although morphologic evidences of malignity are obvious, when the carcinoma in situ stage occurs, aneuploid cells presence in benign dysplasia (CIN I), possibly is an indication of pre-cancerous cells. Böcking et al (1985) and Méhes et al (2004) believe demonstration of DNA ploidy can be used as a quality control in cytology and a morphologic observation in histology.…”

“…Although it's known that the numerous diagnosed dysplasias by cytomorphology and histology are insufficient to detect the preventive malignancy even with a constant preoccupation of inner controls, Amaral et al (2003), Pittoli et al (2003) and Böcking et al (1985) think DNA quantification can be used in different techniques such as Giemsa, Toluidine Blue, Gallocyanine (stain methods), and other coloration techniques. Böhm (1968) efforts have been made towards automatic diagnostic of tumors by flow cytometry to measure nuclear DNA.…”

Squamous Cell Carcinoma of the Upper Aerodigestive Tract: Precursors and Problematic Variants

“…This clinical concern is due to the fact that severe dysplasia is often multifocal and frequently occurs adjacent to or near synchronous foci of invasive carcinoma. Further, this form of dysplasia has a rate of progression to invasive carcinoma that is greater than that of "classic" CIS (20,23,25). A diagnosis of severe dysplasia requires therapeutic intervention, as well as clinical evaluation of the entire UADT to exclude the possible presence of additional foci of dysplasia or carcinoma.…”

Cellular DNA content and prognosis in surgically treated squamous carcinoma of the larynx

“…Of these 12 do not discuss survival (Danes et al, 1987;Ensley et al, 1989;Feinmesser et al, 1990;Franzen et al, 1987a,b;Graessel-Pietrusky & Hornstein, 1982;Hemmer & Kreidler, 1990;Johnson et al, 1985;Kaplan et al, 1986;Kearsley et al, 1990;Olinici & Caluser, 1987;Wilson et al, 1988). Of the remaining 14 articles, four do not state the type of treatment the patient received (Boecking et al, 1985;Chen, 1989;Lampe et al, 1987;Oloffson et al, 1986). In seven reports the patients were treated by a variety of combinations of radiotherapy, chemotherapy or surgery (Feichter et al, 1987;Goldsmith et al, 1986;Goldsmith et al, 1987;Guo et al, 1989;Holm, 1982;Sickle-Santanello et al, 1986;Tytor et al, 1987).…”