Diagnosis Based on 1D Gene Analysis of the Foot-And-Mouth Disease Virus

Abstract

A foot and mouth disease (FMD) is an acute, febrile and high contagious viral disease in many cloven hoofed domestic animals and more than 70 wild ones, resulting in severe finantial loss throughout the world. Choosing the correct seeds for foot-and-mouth disease (FMD) is an important issue in protecting this disease, and it is urgently necessary to establish a plan for vaccination in areas affected by FMD and to protect new foot-and-mouth disease. The genetic diversity and antigenic diversity of foot-and-mouth disease virus (FMDV) make eradication through vaccination difficult. Variable antigenic types exist in different geographic areas, and research projects on existing antigenic types are necessary to select vaccine in such an environment. From this, in this paper, oligonucleotide primers for detection and Selecting Serotype of FMDV were newly designed and synthesized. In addition, the nucleotide sequence of the 1D gene was aligned, the mutation region was determined, and the homology and phylogenetic relationship of the nucleotide sequence were analyzed. The antigenicity of the FMDV type O strains in the Democratic People's Republic of Korea and the correspondence between them were examined. The neutralization reaction was used to examine antigenicity between FMDV to select waxy seeds. To prevent FMD through this primer design and experimental method, the nucleic acid of FMDV was amplified by RT-PCR. Then, the nucleotide sequence of the 1D gene corresponding to the virus VP1 protein was analyzed and compared to select a seed vaccine strain.