Diagnosis of Atypical Neurocysticercosis by Polymerase Chain Reaction Analysis: Case Report

Meri, Taru; Jokiranta, T. Sakari; Granat, Simo; Collander, Fredrika; Valtonen, Matti; Meri, Seppo

doi:10.1086/517790

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…As expected, given previous observations (Meri et al, 1999;Almeida et al, 2006), cysticercal DNA was detected in the CSF of most of the cases (10/16) who had vesicular parasites in their central nervous system. The PCR was most likely to give a positive result when the CSF sample being tested came from a patient with the most severe form of NC, with parasites in the subarachnoid space at the base of the brain or in a ventricle .…”

Section: Discussionsupporting

confidence: 90%

Neurocysticercosis: detection ofTaenia soliumDNA in human cerebrospinal fluid using a semi-nested PCR based on HDP2

Hernández

González

Fleury

et al. 2008

Annals of Tropical Medicine & Parasitology

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Human neurocysticercosis (NC) is caused by Taenia solium larvae lodged in the central nervous system. This disease is usually diagnosed by radiology but the results are not always clear-cut and so immunological assays are often also used. A semi-nested PCR, based on the non-coding HDP2 sequence of T. saginata, has now been developed for detecting DNA from T. solium cysticerci and confirming NC. This PCR, which amplifies a 171-bp T. solium product, allowed the specific detection of just 174 attograms of T. solium DNA. The efficacy of the PCR was tested using cerebrospinal fluid (CSF) from neurological patients, including 46 confirmed Mexican cases of NC and 32 patients from non-endemic Spain. Eighteen of the confirmed cases [including 10 (71%) of the 14 with vesicular extraparenchymal cysticerci and four (17%) of the 24 with damaged cysticerci] and two (33%) of the six patients with 'uncertain' diagnosis (in whom a diagnosis of NC could not be established by radiological and immunological studies) were found PCR-positive. The 36 patients known to have neurological problems other than NC were found PCR-negative. The HDP2 PCR offers a new tool in the diagnosis of NC and in exploring the pathogenesis of this serious disease.

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Section: Discussionsupporting

confidence: 90%

Neurocysticercosis: detection ofTaenia soliumDNA in human cerebrospinal fluid using a semi-nested PCR based on HDP2

Hernández

González

Fleury

et al. 2008

Annals of Tropical Medicine & Parasitology

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“…The use of DNA-based techniques for NCC diagnosis has been reported ( 4 , 10 , 11 ). A recent polymerase chain reaction (PCR) assay study showed excellent sensitivity and specificity in the diagnosis of extraparenchymal NCC ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Neurocysticercosis

et al. 2018

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Background: Neurocysticercosis (NCC) is the most common helminthic infection of the central nervous system (CNS). The diagnosis of NCC is sometimes challenging due to its heterogenous clinical manifestations and the variable sensitivity and specificity of neuroimaging and serological tests.Methods: Next-generation sequencing (NGS) of cerebrospinal fluid (CSF) was used to detect pathogens in patients with clinically suspected CNS infections. A series of patients diagnosed with NCC is reviewed here.Results: Using NGS of CSF, four patients were diagnosed with NCC. The reads corresponding to Taenia solium ranged from 478 to 117,362, with genomic coverage of 0.0564–11.15%. Reads corresponding to T. solium were not found in non-template controls and far exceeded those of the background microorganisms in patients with NCC, facilitating the interpretation of the NGS results.Conclusions: This case series demonstrates that NGS of CSF is promising in the diagnosis of NCC in difficult to diagnose cases. Larger studies are needed in the future.

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“…A stool examination for ova and parasites should be performed in both the patient and his family members. Recently, use of the technique of polymerase chain reaction has been described for the establishment of a diagnosis of NC in a patient with atypical presentation 14 .…”

Section: Discussionmentioning

confidence: 99%

Neurocysticercosis in a preschool-age child: case report

Vasconcelos

Ramos²,

Schwan³

et al. 2000

Arq. Neuro-Psiquiatr.

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-Neurocysticercosis is a frequent cause of epilepsy and other neurologic abnormalities in all age groups, however by virtue of its prolonged incubation period as well as young children's nutritional habits, it is rarely seen in preschool-age children. The objective of this study is to report the case of a 2 ½ year-old child who presented with new-onset seizures. Her diagnostic features, including neuroradiologic findings, are described and compared with the literature. No matter how young he or she may be, every child who presents with newonset seizures or other unexplained neurologic features and whose CT or MRI shows cystic lesions or contrastenhancing rounded lesions should raise a suspicion of neurocysticercosis.KEY WORDS: cysticercosis, CNS, childhood, CT, MRI. Neurocisticercose em uma criança pré-escolar: relato de casoRESUMO -A neurocisticercose é causa freqüente de epilepsia e outras manifestações neurológicas em todas as faixas etárias, mas devido ao seu período de incubação longo e aos hábitos alimentares das crianças pequenas, raramente é vista em pré-escolares. O objetivo deste estudo é descrever o caso de uma criança de 2,5 anos de idade que se apresentou com convulsões de início recente. As manifestações clínicas que levaram ao diagnóstico e os achados neurorradiológicos são descritos e comparados com a literatura. Até mesmo nas menores faixas etárias, toda criança que se apresentar com convulsões ou outras manifestações neurológicas inexplicadas e cuja TC ou RM mostrar lesões císticas ou lesões arredondadas captantes de contraste deve levantar a suspeita de neurocisticercose.

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Diagnosis of Atypical Neurocysticercosis by Polymerase Chain Reaction Analysis: Case Report

Cited by 9 publications

References 4 publications

Neurocysticercosis: detection ofTaenia soliumDNA in human cerebrospinal fluid using a semi-nested PCR based on HDP2

Neurocysticercosis: detection ofTaenia soliumDNA in human cerebrospinal fluid using a semi-nested PCR based on HDP2

Next-Generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Neurocysticercosis

Neurocysticercosis in a preschool-age child: case report

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