Diagnosis of canine visceral leishmaniasis: Biotechnological advances

Gomes, Yara M.; Cavalcanti, Milena Paiva; Lira, Rodrigo Araújo; Abath, Frederico G. C.; Alves, Lêucio Câmara

doi:10.1016/j.tvjl.2006.10.019

Cited by 97 publications

(94 citation statements)

References 70 publications

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“…Leishmania species cause a variety of human and animal diseases, ranging from self-limited cutaneous to the more severe diffuse cutaneous and visceral forms as consequence of the complex host immunological response depending on the invading Leishmania species (Grimaldi and Tesh, 1993;Gomes et al, 2008, Gramiccia M, 2011. Dogs are considered to be the main reservoir of L. infantum constituting part of the epidemiological cycle of human transmission (Acedo-Sánchez et al, 1996;Fisa et al, 1999;Gramiccia, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…frequent clinical signs reported were lymphadenomegaly, weight loss, poor appetite, dermatological lesions, onychogryphosis and to a lesser extent ocular lesions, epistaxis, renal failure, lameness, diarrhea and meningitis (Slappendel, 1988;Ciaramella et al,1997;Vinuelasat al., 2001, Saridomichelakis et al, 2009). The conventional diagnosis of leishmaniosis consists of serological tests like ELISA and immunofluorescence, direct examination of smears after Giemsa staining, culture of parasite and histopathological techniques (Grimaldi and Tesh, 1993;Gomes et al, 2008). These methods are not used for species identification.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, molecular techniques are used to detect and discriminate the species in recent years (Lachaud et al, 2002;Ferroglio et al, 2006;Gramiccia, 2011). The PCR method has advantageous as it is rapid, sensitive and specific (Moreira et al, 2007;Gomes et al, 2008). The aim of this study was to investigate the frequency of canine leishmaniosis in Istanbul (north-western Turkey).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Bilgin

Turan

Yılmaz

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. Leishmania infantum is widespread in Mediterranean countries including Turkey and can cause a serious disease in both humans and dogs. Dogs are considered to be its main reservoirs. The current epidemiological study was carried out in Istanbul for detection of leishmaniosis among dogs. A total of 246 dogs were included in the study. Twenty one (8.54%) blood samples were found to be positive based on PCR diagnosis, using primers specific for the kinetoplast DNA of Leishmania. Infection rate was 6.51% in 169 dogs living in shelters and 12.99% in 77 client-owned dogs. The rate was significantly (p<0.001) higher in the dogs (37.93%) presenting one or more clinical symptoms which may be attributable to leishmaniosis, than in the asymptomatic dogs (4.61%). Eleven (52.4%) of 21 PCR positive dogs presented clinical symptoms whereas the rest of the dogs (47.62%) were asymptomatic. The major clinical sign in PCR positive animals was dermatological problems. Amongst PCR positive dogs, skin lesions were present in 11, weight loss in 2, lymphadenopathy in 2, epistaxis in 2 and lethargy in 2 dogs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Bilgin

Turan

Yılmaz

et al. 2018

J Hellenic Vet Med Soc

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“…Conventional serological tests are limited due to cross-reactions with other parasitological diseases. Thus, a number of studies have discussed polymerase chain reaction (PCR) and immunoassays based on the use of recombinants to enhance the sensitivity and specificity of the diagnosis of CVL (BENSOUSSAN et al, 2006;GOMES et al, 2008). Invasive methods have been used for the diagnosis of this disease based on clinical samples, such as aspirates of bone marrow, spleen tissue and lymph nodes (GOMES et al, 2008), but such methods are distressing for animals.…”

Section: Introductionmentioning

confidence: 99%

Leishmania (infantum) chagasi in canine urinary sediment

Mendonça

Batista

Alves

2015

Rev. Bras. Parasitol. Vet.

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Canine visceral leishmaniasis (CVL) is difficult to diagnosis, mainly due to the presence of asymptomatic animals, the diversity of clinical symptoms and the difficulty in obtaining diagnostic evidence of high sensitivity and specificity. The purpose of this study was to diagnose CVL in urinary sediment of 70 dogs of different breeds, sexes and ages from the veterinary hospital of the Federal University of Piauí and Zoonosis Control Center of Teresina, Brazil. The serological tests were TR DPP  for CVL and enzyme-linked immunosorbent assay (ELISA) for CVL, parasitological exams of bone marrow and lymph nodes and urine sediment cultures. Leishmania was detected in the bone marrow and/or lymph node of 61.0% of the animals (43/70), and urine sediment culture was positive in 9.30% (4/43) of these animals. In the serological exams, 70.0% (49/70) were reactive using the DPP and 78.2% (55/70) were reactive using ELISA. The goal of this study was to diagnose the presence of L. (infantum) chagasi in a culture of urinary sediment.Keywords: Leishmania, urine, dogs, CVL, TR DPP  . ResumoA leishmaniose visceral canina (LVC) é uma doença de difícil diagnóstico. Principalmente devido à presença de animais assintomáticos, a diversidade da sintomatologia clínica apresentada e também pela dificuldade em se obter uma prova diagnóstica que reúna alta sensibilidade e especificidade. O objetivo deste trabalho foi relatar a presença de L. (infantum) chagasi em meio de cultura, utilizando-se sedimento urinário. Foram utilizados neste experimento, 70 cães provenientes do Hospital Veterinário Universitário da Universidade Federal do Piauí e do Centro de Controle de Zoonoses de Teresina, com raça, sexo e idade variada. Foram realizados exames sorológicos: TR DPP  Leishmaniose Visceral Canina (DPP) e Ensaio Imunoenzimático Leishmaniose Visceral Canina (ELISA), exames parasitológicos de amostras de medula e/ou linfonodo e cultura de sedimento urinário. Em 61,0% (43/70) dos animais estudados, observou-se presença de Leishmania em medula e/ou linfonodo, e destes 9,30% (4/43) foram positivos na cultura de sedimento urinário. Nos exames sorológicos, 70,0% (49/70) dos animais apresentavam-se reativos no DPP e 78,2% (55/70) no ELISA. Pode-se concluir, neste estudo, que é possível diagnosticar a LVC por meio da cultura de sedimento urinário.

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“…Moreover, such methods are invasive. Conventional serological techniques are limited by cross-reactivity with other parasitic diseases and because several technical procedures have not been standardized (2). However, serological methods are still widely employed in epidemiological surveys.…”

Section: Introductionmentioning

confidence: 99%

Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review

Paiva-Cavalcanti¹,

Régis-da-Silva²,

Gomes³

2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. Moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.

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Diagnosis of canine visceral leishmaniasis: Biotechnological advances

Cited by 97 publications

References 70 publications

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Leishmania (infantum) chagasi in canine urinary sediment

Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review

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