Diagnosis of cypress pollen allergy: in vivo and in vitro standardization of a Juniperus ashei pollen extract

Hrabina, M.; Dumur, J.-P.; Sicard, H.; Viatte, Auguste; André, C.

doi:10.1034/j.1398-9995.2003.00247.x

Cited by 33 publications

(24 citation statements)

References 10 publications

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“…A review of the literature revealed only very few double-blind, placebo-controlled studies of cypress pollen immunotherapy, and none of the published studies were performed with a standardized allergen extract, as this type of extract has only recently become available [2]. These few clinical studies were also conducted with allergen extracts derived from pollen of related species.…”

Section: Discussionmentioning

confidence: 99%

“…The allergen extract was standardized with reference to an in-house reference preparation by RAST inhibition, isoelectric focusing and immunoblotting for the determination of allergenic potency [2]. The biological activity of the in-house reference preparation was determined by skin prick test, and potency was expressed as the arbitrary index of reactivity (IR).…”

Section: Methodsmentioning

confidence: 99%

“…The poor quality of allergen extracts used for skin prick tests made the diagnosis relatively difficult, but this was facilitated by standardization of a Juniperus ashei extract [2]. Comparison of three 1/20 (m/V) Cupressus sempervirens , Cupressus arizonica and J. ashei allergen extracts showed a much higher cutaneous reactivity for J. ashei than for the other cypress species.…”

Section: Introductionmentioning

confidence: 99%

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Immunotherapy with an Aluminum Hydroxide-Adsorbed Juniperus ashei Foreign Pollen Extract in Seasonal Indigenous Cypress Pollen Rhinoconjunctivitis

Charpin¹,

Gouitaa²,

Dron‐Gonzalvez³

et al. 2007

Int Arch Allergy Immunol

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Background:The efficacy of standardized Juniperus ashei extract was assessed in patients with allergic rhinoconjunctivitis due to European cypress pollens. Methods:Forty adults with European cypress-allergic rhinoconjunctivitis were randomized to receive immunotherapy or a matched placebo. Specific immunotherapy was performed with a standardized, aluminum hydroxide-adsorbed J. ashei extract with a potency of 100 IR (arbitrary index of reactivity) containing 54 µg of Jun a 1/ml (Alustal, Stallergenes, France). Subcutaneous injections started in October 2000. The maintenance dose was 0.30 ml of the 100-IR concentration per month. Rhinitis and conjunctivitis symptoms were rated according to a 4-point score. Results: Seventeen patients from the treated group and 15 patients from the placebo group completed year 2001; 14 in each group completed year 2002. A statistically significant improvement (41%, p < 0.02) in the conjunctivitis symptom score was observed in actively treated patients compared to the placebo group at the peak of the 2001 pollen season. Improvement in rhinitis (17%) was not significant. This significant improvement was greater at the peak of the 2002 pollen season (63%, p < 0.01). Conclusions: This study therefore indirectly validates the concept of treatment by major allergen because J. ashei is absent from the region in which this study was conducted.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunotherapy with an Aluminum Hydroxide-Adsorbed Juniperus ashei Foreign Pollen Extract in Seasonal Indigenous Cypress Pollen Rhinoconjunctivitis

Charpin¹,

Gouitaa²,

Dron‐Gonzalvez³

et al. 2007

Int Arch Allergy Immunol

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“…Two-dimensional (2-D) electrophoresis was performed as previously described [24]. Briefly, Immobiline DryStrip pH 3–10, 11 cm (GE Healthcare), was rehydrated with a rehydration solution (thiourea, urea, CHAPS 4%, glycerol 10%, dithiotreitol and IPG buffer) and ash pollen IHRP was applied.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblotting was performed as described elsewhere [24]. Briefly, proteins from 1-D or 2-D gels were transferred onto 0.45 µm pore size nitrocellulose membranes and stained with Ponceau-S red to demonstrate successful transfer of proteins.…”

Section: Methodsmentioning

confidence: 99%

Standardization of an Ash (Fraxinus excelsior) Pollen Allergen Extract

Hrabina

Purohit²,

Oster³

et al. 2006

Int Arch Allergy Immunol

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Background: Ash tree (Fraxinus excelsior) is the main representative of the Oleaceae family in temperate zones. Diagnosis of ash pollen allergy is made difficult due to (1) an overlapping pollinization period with Betulaceae, (2) non-inclusion in current diagnostic assays, and (3) some cross- reactivity with minor allergens from Betulaceae. The aim of this study was to calibrate an ash pollen in-house reference preparation (IHRP) in allergic patients in order to produce standardized products for diagnosis and immunotherapy purposes. Methods: Ash pollen IHRP was extracted, ultrafiltered and freeze dried. Allergens in the extract were detected after 2-dimensional PAGE using specific sera and a monoclonal antibody. The Fra e 1 content of IHRP was evaluated by quantitative immunoprint. Forty-eight subjects from the North-East of France exhibiting clinical symptoms, a positive skin test and specific IgE levels ≧class 2 to ash pollen were recruited. IgE immunoprints were performed to select patients sensitized to the ash Fra e 1 allergen as opposed to cross-reacting allergens. Serial 10-fold dilutions of the IHRP were tested by skin prick tests in order to determine the concentration inducing a geometrical mean wheal diameter of 7 mm, said to correspond to an index of reactivity (IR) of 100 per millilitre. Results: IgE-reactive molecules in IHRP comprise Fra e 1, Fra e 2, a 9-kDa molecule (presumably Fra e 3), as well as a doublet at 15 kDa and high molecular weight allergens. The 100 IR concentration of IHRP inducing a geometrical mean wheal diameter of 7 mm in 22 patients sensitized to Fra e 1 corresponds to the 1/126 (w/v) extraction ratio (i.e. 259 µg/ml of protein by Bradford) and contains 17 µg/ml of Fra e 1. The variability in total activity of 5 batches of standardized extracts was found to be significantly reduced when compared with 7 non-standardized extracts. Conclusion: An ash pollen IHRP was defined and molecularly characterized. Its successful standardization at 100 IR/ml in patients specifically sensitized to Fra e 1 allowed a skin reactivity-based calibration in properly diagnosed patients. Such a standardized ash pollen extract is a reliable tool to support immunotherapy of ash pollen allergy.

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“Omics” and Biomedical Applications

Ferranti

Nitride

Gallo

2015

Analytical Separation Science

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Extensive research has shown that data generated by the various omic technologies represent a unique resource for biologists. This chapter explores how classical approaches to study biological processes, such as those used in chemistry, biochemistry, immunology, and genetics, intersect with the new omics technologies to advance the understanding of biological systems by examination of case studies of general interest for researchers. It explores the development of simple and robust methods for understanding the meaning of omics data in biomedical sciences. The chapter highlights that instead of looking at one potential biomarker at a time, the new techniques in genomics, transcriptomics, proteomics, metabolomics, lipidomics, and glycomics have allowed researchers to identify patterns in the changes of many genes and compounds that correlate with disease state. Mass spectrometry (MS) and spectroscopic techniques are the key analytical platforms on which the emerging omics technologies are based

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Diagnosis of cypress pollen allergy: in vivo and in vitro standardization of a Juniperus ashei pollen extract

Abstract: Standardized Ja pollen extract is therefore a very appropriate tool for the in vivo diagnosis of cypress pollen allergy and good candidate for specific immunotherapy.

Cited by 33 publications

References 10 publications

Immunotherapy with an Aluminum Hydroxide-Adsorbed <i>Juniperus ashei</i> Foreign Pollen Extract in Seasonal Indigenous Cypress Pollen Rhinoconjunctivitis

Immunotherapy with an Aluminum Hydroxide-Adsorbed <i>Juniperus ashei</i> Foreign Pollen Extract in Seasonal Indigenous Cypress Pollen Rhinoconjunctivitis

Standardization of an Ash <i>(Fraxinus excelsior) </i>Pollen Allergen Extract

“Omics” and Biomedical Applications

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