Diagnosis of Cytomegalovirus (CMV) Polyradiculopathy and Documentation of In Vivo Anti‐CMV Activity in Cerebrospinal Fluid by Using Branched DNA Signal Amplification and Antigen Assays

Flood, Jennifer; Drew, W. Lawrence; Miner, Richard C.; Jekic-McMullen, Dragana; Shen, Lu-Ping; Kolberg, Janice A.; Garve, Janice; Follansbee, Stephen; Poscher, Margaret E.

doi:10.1086/514051

Cited by 24 publications

(15 citation statements)

References 10 publications

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“…Also, pp65 antigenemia assay level of >100nuclei/200,000 cells has been associated with the occurrence CMV disease in 63% of patients, compared to 3% in antigenemia negative patients [160]. Additionally, the pp65 antigenemia from the CSF can be successfully used in confirmation of CMV polyradiculomyelopathy seen almost exclusively in AIDS patients [167, 168]. …”

Section: Infections In Immunocompromised Hostsmentioning

confidence: 99%

Overview of the Diagnosis of Cytomegalovirus Infection

Ross¹,

Novák²,

Pati³

et al. 2011

IDDT

212

177

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Cytomegalovirus (CMV) is recognized as the most common congenital viral infection in humans and an important cause of morbidity and mortality in immunocompromised hosts. This recognition of the clinical importance of invasive CMV disease in the setting of immunodeficiency and in children with congenital CMV infection has led to the development of new diagnostic procedures for the rapid identification of immunocompromised individuals with CMV disease, as well as fetuses and infants with congenital infection. Diagnosis of acute maternal CMV infection by the presence of IgM and low IgG avidity requires confirmation of fetal infection which is typically performed by CMV PCR of the amniotic fluid. Viral culture of the urine and saliva obtained within the first two weeks of life continue to be the gold standard for diagnosis of congenitally infected infants. PCR assays of dried blood spots from infants have not been shown to have sufficient sensitivity for the identification of most infants with congenital CMV infection. However, saliva PCR assays are currently being assessed as a useful screening method for congenital CMV infection. In the immunocompromised host, newer rapid diagnostic assays such as pp65 antigenemia and real-time CMV PCR of blood or plasma have allowed for preemptive treatment reducing morbidity and mortality. However, lack of standardized real-time PCR protocols hinders the comparison of the data across different centers and the development of uniform guidelines for the management of invasive CMV infections in immunocompromised individuals.

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Section: Infections In Immunocompromised Hostsmentioning

confidence: 99%

Overview of the Diagnosis of Cytomegalovirus Infection

Ross¹,

Novák²,

Pati³

et al. 2011

IDDT

212

177

View full text Add to dashboard Cite

show abstract

“…We performed a very limited assessment of mono versus combined therapy in patients with CMV CNS disease, undergoing serial CSF examinations (Flood et al, 1997). Utilizing the bDNA assay, we compared the rate of decline of CMV signal in patients receiving GCV 10 mg/kg/d versus GCV 10 mgm/kg/d plus FOS 180 mg/kg/d.…”

Section: Therapy Of CMV Viremic Diseasementioning

confidence: 99%

Is combination antiviral therapy for CMV superior to monotherapy?

Drew¹

2006

Journal of Clinical Virology

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“…The branched DNA assay is based on signal amplification rather than target-amplification (Urdea, 1994). This assay has been used to measure CMV DNA and HIV-1 RNA levels in the CSF of HIV-infected patients (Flood et al, 1997;Stingele et al, 2001).…”

Section: Na Amplification Techniquesmentioning

confidence: 99%

Molecular analysis of cerebrospinal fluid in viral diseases of the central nervous system

Cinque

Bossolasco

Lundkvist

2003

Journal of Clinical Virology

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The use of nucleic acid (NA) amplification techniques has transformed the diagnosis of viral infections of the central nervous system (CNS). Because of their enhanced sensitivity, these methods enable detection of even low amounts of viral genomes in cerebrospinal fluid. Following more than 10 years of experience, the polymerase chain reaction or other NA-based amplification techniques are nowadays performed in most diagnostic laboratories and have become the test of choice for the diagnosis of several viral CNS infections, such as herpes encephalitis, enterovirus meningitis and other viral infections occurring in human immunodeficiency virus-infected persons. Furthermore, they have been useful to establish a viral etiology in neurological syndromes of dubious origin and to recognise unusual or poorly characterised CNS diseases. Quantitative methods have provided a valuable additional tool for clinical management of these diseases, whereas post-amplification techniques have enabled precise genome characterisation. Current efforts are aiming at further improvement of the diagnostic efficiency of molecular techniques, their speed and standardisation, and to reduce the costs. The most relevant NA amplification strategies and clinical applications of to date will be the object of this review.

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Diagnosis of Cytomegalovirus (CMV) Polyradiculopathy and Documentation of In Vivo Anti‐CMV Activity in Cerebrospinal Fluid by Using Branched DNA Signal Amplification and Antigen Assays

Cited by 24 publications

References 10 publications

Overview of the Diagnosis of Cytomegalovirus Infection

Overview of the Diagnosis of Cytomegalovirus Infection

Is combination antiviral therapy for CMV superior to monotherapy?

Molecular analysis of cerebrospinal fluid in viral diseases of the central nervous system

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