Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva

Balmaseda, Ángel; Guzmán, María G.; Hammond, Samantha N.; Robleto, Guillermo; Flores, C. A. S.; Téllez, Yolanda; Videa, Elsa; Saborío, Saira; Pérez, Lissette; Sandoval, Erick; Rodriguez, Yoryelin; Harris, Eva

doi:10.1128/cdli.10.2.317-322.2003

Cited by 129 publications

(123 citation statements)

References 20 publications

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“…Compared to the haemagglutination inhibition assay as the gold standard, MAC-ELISA shows a sensitivity and specificity of 90% and 98%, respectively, in samples collected after 5 days of fever 55 . In addition to serum, dengue-specific IgM can be detected in whole blood on filter paper (sensitivity 98.1% and specificity 98.5%) 77,78 and in saliva (sensitivity 90.3% and specificity 92.0%) 79 , but not in urine 68 . More than 50 commercial kits are available with variable sensitivity and specificity 65,[80][81][82] .…”

Section: Mac-elisamentioning

confidence: 99%

Dengue: a continuing global threat

et al. 2010

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Dengue fever and dengue haemorrhagic fever are important arthropod-borne viral diseases. Each year, there are ~50 million dengue infections and ~500,000 individuals are hospitalized with dengue haemorrhagic fever, mainly in Southeast Asia, the Pacific and the Americas. Illness is produced by any of the four dengue virus serotypes. A global strategy aimed at increasing the capacity for surveillance and outbreak response, changing behaviours and reducing the disease burden using integrated vector management in conjunction with early and accurate diagnosis has been advocated. Antiviral drugs and vaccines that are currently under development could also make an important contribution to dengue control in the future.Dengue is the most important arthropod-borne viral infection of humans. Worldwide, an estimated 2.5 billion people are at risk of infection, approximately 975 million of whom live in urban areas in tropical and sub-tropical countries in Southeast Asia, the Pacific and the Americas 1 . Transmission also occurs in Africa and the Eastern Mediterranean, and rural Europe PMC Funders GroupAuthor Manuscript Nat Rev Microbiol. Author manuscript; available in PMC 2015 February 19. Published in final edited form as:Nat Rev Microbiol. 2010 December ; 8(12 0): S7-16. doi:10.1038/nrmicro2460. Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts communities are increasingly being affected. It is estimated that more than 50 million infections occur each year, including 500,000 hospitalizations for dengue haemorrhagic fever, mainly among children, with the case fatality rate exceeding 5% in some areas [1][2][3][4] . The geographical areas in which dengue transmission occurs have expanded in recent years (FIG. 1), and all four dengue virus serotypes (DENV-1-4) are now circulating in Asia, Africa and the Americas, a dramatically different scenario from that which prevailed 20 or 30 years ago (FIG. 2). The molecular epidemiology of these serotypes has been studied in an attempt to understand their evolutionary relationships 11 .This Review will provide an update on our understanding of the pathogenesis of this successful pathogen, how we diagnose and control infection and the progress that has been made in vaccine development. Dengue virus pathogenesisDengue viruses belong to the genus flavivirus within the Flaviviridae family. DENV-1-4 evolved in non-human primates from a common ancestor and each entered the urban cycle independently an estimated 500-1,000 years ago 12 . The virion comprises a spherical particle, 40-50 nm in diameter, with a lipopolysaccharide envelope. The positive singlestrand RNA genome (FIG. 3), which is approximately 11 kb in length, has a single open reading frame that encodes three structural proteins -the capsid (C), membrane (M) and envelope (E) glycoproteins -and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5 [18][19][20] . ADE occurs when mononuclear phagocytes are infected through their Fc receptors by immune complexes that form between DE...

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Section: Mac-elisamentioning

confidence: 99%

Dengue: a continuing global threat

et al. 2010

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“…DENV-specific IgM was detected by using a single-dilution IgM-capture enzyme-linked immunosorbent assay (ELISA), 13 and total antibodies to DENV were measured by using an inhibition ELISA 4,14,15 and analyzed using the method of Reed and Muench 16 to confirm serologic diagnosis and to distinguish primary and secondary DENV infections. Both serologic assays were performed on paired day 1 and day 14 serum samples.…”

Section: Methodsmentioning

confidence: 99%

Index Cluster Study of Dengue Virus Infection in Nicaragua

Reyes¹,

Mercado²,

Standish³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Traditional study designs do not identify acute asymptomatic or pre-symptomatic dengue virus (DENV) infections, thus limiting our understanding of immunologic and viral factors that modulate infection outcome. In the 2006 and 2007 dengue seasons, we conducted a pilot index cluster study in Managua, Nicaragua, in which 442 persons living within 50 meters of 22 index cases identified through an ongoing pediatric cohort study were evaluated for DENV infection. Post-enrollment and pre-enrollment DENV infections were confirmed in 12 (2.7%) and 19 (4.3%) contacts, respectively. Five (42%) post-enrollment infections were asymptomatic, and DENV-2 was identified in 9 (75%) infections. Phylogenetic analysis with full-length DENV genomic sequence from contacts, index cases, and cohort dengue cases indicated focal transmission and infection outside the local area. We demonstrate the feasibility of identification of acute asymptomatic and pre-symptomatic cases in urban Latin America, the first report of such a study in the Americas, and identify age and concomitant immunity to DENV of contacts as a key factor in index cluster study design.

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“…High homology between dengue serotypes and recognition of common epitopes, during a sequential could explain the IgM levels detected in our cases. Dengue IgA antibody detection in serum samples from individuals with dengue infections have been proposed as a serological marker for diagnosis [9] [39] and as an indicator of recent infection [40] [41]. In this study, highest IgA ratios were observed for secondary D and SD cases and mainly in samples collected after defervescence day.…”

Section: Discussionmentioning

confidence: 56%

“…The IgA has been commented with an analogous behaviour to IgG antibody. In primary infection must be detectable after the release of IgM, while in secondary infection IgA appear early in acute phase by the common epitopes recognition by memory B cell clones circulating on blood [40] [42]. The induction of high-rate IgA levels requires a group of factors which must act synergistically in the Ig production predominantly toward IgA isotype: engagement of CD40 (B cells) by CD40 ligand (CD40L, Ag-activated CD4+ T cells) which determines class switch gene recombination; TGF-ß secreted by CD40L-induced B cells is required for switching to IgA; the presence of IL-10 increasing both the number of IgA lymphocytes and the amount of secreted IgA.…”

Section: Discussionmentioning

confidence: 99%

Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

Ruiz¹,

Vázquez²,

Villegas³

et al. 2017

OALib

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Latest estimates suggest 390 million infections of dengue occur each year, of which 100 million results in symptomatic disease. The new clinical classification proposed by WHO-TRD 2009 simplifies dengue fever (DF) into dengue (D) and severe dengue (SD) with an intermediary group of "dengue with warning signs" (DWS). Early, sensitive and specific diagnosis of dengue is needed for appropriate patient management as well as for early epidemic detection. Virological and serological markers in sera from dengue confirmed patients from Venezuela and Nicaragua were evaluated. A total of 232 sera collected in the acute (days 1 to 5 of fever onset) and convalescent (days 7 to 14) phases of illness were tested by Platelia NS1 antigen ELISA and IgM, IgA and IgE capture ELISA methods. NS1 was detected with highest OD values in the acute phase for primary and secondary cases. IgM was detected in primary and secondary groups but the highest OD figures were observed after defervescence day. IgA and IgE detection were almost absent for primary cases, contrary to secondary cases which showed higher values but after defervescence day. NS1 could be used as effective diagnostic marker in the acute phase of illness for primary and secondary dengue infections. IgM detection is still an invaluable tool for routine dengue diagnosis but delay. IgA detection could define secondary infection in early phase of illness. IgA/IgE could be evaluated as prognostic markers associated to SD cases.

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Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva

Cited by 129 publications

References 20 publications

Dengue: a continuing global threat

Dengue: a continuing global threat

Index Cluster Study of Dengue Virus Infection in Nicaragua

Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

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