Diagnosis of Helicobacter pylori: comparison of an urease test, histological visualization of curved bacteria and culture

Abstract

Helicobacter pylori was investigated in 189 patients for culture, microscopic visualization of campylobacter-like organisms (CLO) and a ten minute urease test. In 136 (72%) the bacteria was isolated, and in 98 of them CLO were histologically detected. Specificity, sensitivity, positive and negative predictive values of microscopic visualization of CLO were: 0.77, 0.73, 0.97 and 0.51, respectively; 98 culture-positive patients were urease test positive. Specificity, sensitivity, positive and negative predictive values of the urease test were: 0.83, 0.72, 0.92 and 0.54, respectively. Comparing the urease test with culture of H. pylori combined with microscopic visualization of CLO, its specificity, sensitivity, positive and negative predictive values were: 0.95, 0.71, 0.98 and 0.48, respectively. Probably, these values are not real, since bacteria different from H. pylori could be misclassified as CLO.

Antibodies to Helicobacter pylori in dyspeptic patients, asymptomatic adults, and children from Costa Rica

“…H. pylori infection has been associated with gastric injury and an increased risk of carcinoma (1,3,22). Two earlier studies in Costa Rica reported isolation of H. pylori in 62 out of 92 (67%) and 136 out of 189 (72%) adult dyspeptic patients respectively (8,9). However, these prevalence values relate to dyspeptic patients, and no information is available regarding the general population.…”

In vitro and In vivo Anti-Helicobacter pylori Activities of Centella asiatica Leaf Extract

“…One day after the final administration, the mice were killed and their gastric mucosa was biopsied for the detection of H. pylori . The presence of H. pylori in gastric mucosa was confirmed by direct bacterial culture, as previously described ( 25 ). For culturing, tissue was rubbed across the surface of a plate containing a selective medium of 10% bovine serum, 6 μg/mL vancomycin, 5 μg/mL trimethroprim, 10 μg/mL polymyxin B, 200 μg/mL bacitracin, 8 μg/mL amphotericin B, and 10 μg/mL nalidixic acid.…”

Anti-Helicobacter pylori Properties of GutGardTM

“…The presence of H. pylori in gastric mucosa was confirmed by direct bacterial culture, as described previously (25). For culturing, the tissue was rubbed across the surface of a plate with selective medium containing 10% bovine serum, 6 μg/mL vancomycin, 5 μg/mL tri-methroprim, 10 μg/mL polymyxin B, 200 μg/mL baci-tracin, 8 μg/mL amphotericin B and 10 μg/mL nalidixic acid.…”