Diagnosis of Newcastle disease in broiler by histopathology and immunohistochemistry

Brar, R. S.; Leishangthem, Geeta Devi; Gadhave, P. D.; Singh, Namita; Banga, H. S.; Mahajan, Vineet; Sodhi, Simrinder Singh

doi:10.5958/0973-970x.2017.00014.1

Cited by 7 publications

(9 citation statements)

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“…In the current study, NDV nucleoprotein immunolabeling revealed its wide systemic distribution, mainly in the lining epithelium of the conjunctiva, nostril, pulmonary alveoli, bronchial, kidney tubules, intestines, and bursa, in addition to hepatocytes, inflammatory cells (mainly macrophages and lymphocytes), and various neural cells and glia cells of the central nervous system. These findings are in line with some previous studies [ 20 , 49 , 77 ]. However, it should be borne in mind that the immunohistochemical expression of NDV nucleoprotein showed a variable degree of intensity among the examined organs in relation to the route of inoculation, along with more intense immunohistochemical expression of NDV nucleoprotein in inflammatory and epithelial cells recorded in the intranasal and mixed oculo-nasal inoculated groups.…”

Section: Discussionsupporting

confidence: 94%

“…Immunohistochemical staining is widely accepted as an accurate, quick, and economic technique for the identification of the virus and confirmation of the diagnosis of ND [ 69 ]. Importantly, ND viral antigens are mainly localized in the cytoplasm and nuclei of necrotic cells of the various organs, as well as in the epithelia, circulating monocytes, and alveolar macrophages in the lungs [ 77 ] . In the current study, NDV nucleoprotein immunolabeling revealed its wide systemic distribution, mainly in the lining epithelium of the conjunctiva, nostril, pulmonary alveoli, bronchial, kidney tubules, intestines, and bursa, in addition to hepatocytes, inflammatory cells (mainly macrophages and lymphocytes), and various neural cells and glia cells of the central nervous system.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

EL-Morshidy¹,

Abdo

Elmahallawy

et al. 2021

Animals

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Newcastle disease virus (NDV) remains a constant threat to the poultry industry. There is scarce information concerning the pathogenicity and genetic characteristics of the circulating velogenic Newcastle disease virus (NDV) in Egypt. In the present work, NDV was screened from tracheal swabs collected from several broiler chicken farms (N = 12) in Dakahlia Governorate, Egypt. Real-time reverse transcriptase polymerase chain reaction (RRT-PCR) was used for screening of velogenic and mesogenic NDV strains through targeting F gene fragment amplification, followed by sequencing of the resulting PCR products. The identified strain, namely, NDV-CH-EGYPT-F42-DAKAHLIA-2019, was isolated and titrated in the allantoic cavity of 10 day old specific pathogen-free (SPF) embryonated chicken eggs (ECEs), and then their virulence was determined by mean death time (MDT) and intracerebral pathogenicity index (ICPI). The pathogenicity of the identified velogenic NDV strain was also assessed in 28 day old chickens using different inoculation routes as follows: intraocular, choanal slit, intranasal routes, and a combination of both intranasal and intraocular routes. In addition, sera were collected 5 and 10 days post inoculation (pi) for the detection of NDV antibodies by hemagglutination inhibition test (HI), and tissue samples from different organs were collected for histopathological and immunohistochemical examination. A series of different clinical signs and postmortem lesions were recorded with the various routes. Interestingly, histopathology and immunohistochemistry for NDV nucleoprotein displayed widespread systemic distribution. The intensity of viral nucleoprotein immunolabeling was detected within different cells including the epithelial and endothelium lining, as well as macrophages. The onset, distribution, and severity of the observed lesions were remarkably different between various inoculation routes. Collectively, a time-course comparative pathogenesis study of NDV infection demonstrated the role of different routes in the pathogenicity of NDV. The intranasal challenge was associated with a prominent increase in NDV lesions, whereas the choanal slit route was the route least accompanied by severe NDV pathological findings. Clearly, the present findings might be helpful for implementation of proper vaccination strategies against NDV.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

EL-Morshidy¹,

Abdo

Elmahallawy

et al. 2021

Animals

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“…The most commonly observed postmortem lesions in our farms were petechiae in the proventriculus or proventriculitis, tracheitis, and enteritis. These findings match with the observations of Yune and Abdela [11], De Oliveira Torres Carrasco et al [18], Brown and Bevins [17], Brar et al [19], Crespo et al [20], and Talha et al [21].…”

Section: Discussionsupporting

confidence: 92%

Serological, clinical, and risk factors of the Newcastle disease on broilers flocks in Algeria

et al. 2019

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Aim: The work aimed at studying the serological and clinical factors, as well as the risk factors of the Newcastle disease (ND) on broilers herds in Algeria. Materials and Methods: A sample of 1248 birds was randomly selected from 52 broiler flocks. We took blood samples from each bird at the level of the wing vein area where an indirect enzyme-linked immunosorbent assay technique was carried out through the use of an IDvet kit. Results: The flocks showed 82.69% of seroprevalence. Clinically speaking, the most common symptoms were sneezing, rale, greenish diarrhea, torticollis, and motor discords. Most commonly observed postmortem lesions were the proventriculitis, tracheitis, and enteritis. Especially, the caeca are hemorrhagic. The scores show the effect of risk factors. There was a significant effect on the mortality, the hygiene and vaccination groups on antibody titers in time 2. The antibody titers were elevated in the herd that recorded a high mortality (more than 10%) compared with those which recorded a low mortality (<10%) (p=0.002). Therefore, the antibody titers were elevated in herds with bad hygiene, compared with the ones with good hygiene (p=0.04). At last, when broiler chicken were not boosted by ND vaccine, flocks appeared to be more seropositive (p=0.02). Conclusion: The serological survey conducted in this study provided an important scope for ND as a dominant viral disease in broilers. Many factors are responsible for the onset of these diseases; correct biosecurity measures are needed to reduce the impact of this pathology in poultry farms.

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“…In this study, the microscopic lesions showed hemorrhages and necrosis in the mucosa and submucosa of proventiculus, desquamation of proventriculus epithelial layer, globular destruction of proventiculas surface, hyperemia of the muscularis layer and infiltration of inflammatory cells to submucosal layer of proventriculus glands, intestinal villi were blunted and necrosis of sub mucosa and mononuclear cell infiltration in the lamina propria, decreased number of lymphocytes in periarterial lymphotic sheath of spleen, hemorrhages, congestion and necrosis in epithelial mucosal cell of spleen, degeneration and multifocal necrosis in liver of broiler chicken, while in layer chicken they were accompanied by perihepatitis, hemorrhage, necrosis, and multifocal inflammatory cell infiltration in liver, Congestion, edema, and mononuclear inflammatory infiltration at the inside and the wall of alveoli, epicarditis and myocarditis, myocardium degeneration of heart, congestion, hemorrhage, mononuclear inflammatory cell infiltration in subepithelial layer of trachea which have been already reported by Etriwati et al (2017), Brar et al (2017) and Pabitra et al (2016).…”

Section: Prevalence Of Nd In Vaccinated Chickens Having Specific Hi Asupporting

confidence: 58%

Humoral immune response against Newcastle disease virus and its pathological changes in vaccinated chickens in selected areas of Barisal district in Bangladesh

Sumona¹,

Alam²,

Arafat³

et al. 2020

Asian J. Med. Biol. Res

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The objectives of this study were to evaluate humoral immunity against Newcastle disease (ND) virus in vaccinated chickens in terms of serum HI antibody titers in broiler and layer chickens and to determine pathological changes in vaccinated chickens. A total of 240 serum samples were collected from broiler (135) and layer (105) chickens from Barishal Sadar, Babugonj and Gournadi Upazilas of Barishal district in Bangladesh after two weeks of scheduled vaccination programme. The overall detection of Hemagglutination inhibition (HI) antibodies against Newcastle disease virus was 62.2% in broiler and 83.8% in layer chickens. The percentages of specific HI antibody titer in broiler chickens were 57.5, 70 and 48 in 1-2 weeks, 3-4 weeks, and 4-above weeks age groups respectively where in layer chickens were 85, 80, 90, 80 and 80 in 15-24 weeks, 25-34 weeks, 35-44 weeks, 45-54 weeks and > 55 weeks of age groups respectively. For both broiler and layer chicken’s protective antibody titers were found higher in adult than in young chickens. Out of 240 samples HI titers of 172 (71.67%) samples were found at protective level, 42 (17.5%) samples were at marginal level and 26 (10.83%) samples were below protective level. Among 26 samples of non-protective level, 21 were broiler and 5 were layer chicken. Out of 26 samples, 10 (38.46%) were found apparently infected with NDV where 8 (38.09%) were broiler and 2 (40%) were layer. The apparently infected birds were diagnosed on the basis of postmortem findings and histopathological lesions. Results of the present investigation may help to design appropriate vaccination schedule for ND in broiler and layer chickens and thus to protect chickens from ND in field condition. Asian J. Med. Biol. Res. June 2020, 6(2): 155-167

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Diagnosis of Newcastle disease in broiler by histopathology and immunohistochemistry

Cited by 7 publications

References 0 publications

Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

Serological, clinical, and risk factors of the Newcastle disease on broilers flocks in Algeria

Humoral immune response against Newcastle disease virus and its pathological changes in vaccinated chickens in selected areas of Barisal district in Bangladesh

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