Diagnosis of Plant Pathogens and Implications for Plant Quarantine: A Risk Assessment Perspective

Rossi, Vittorio; Candresse, Thierry; Jeger, Michael; Manceau, Charles; Urek, Gregor; Stancanelli, Giuseppe

doi:10.1007/978-94-017-9020-8_12

Cited by 5 publications

(4 citation statements)

References 89 publications

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“…Plant symptoms observation, the first step in pest management, is carried out using optical methods (10). Detection and/or identification of pests actually represent critical steps for the appropriate application of phytosanitary measures as illustrated in a range of international documents (11). International Standards for Phytosanitary Measures (ISPM) No.…”

Section: Bacterial Pathogen Detection and Identificationmentioning

confidence: 99%

The diagnosis of plant pathogenic bacteria a state of art

2018

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Plant protection plays an important role in agriculture for the food quality and quantity. The diagnosis of plant diseases and the identification of the pathogens are essential prerequisites for their understanding and control. Among the plant pests, the bacterial pathogens have devastating effects on plant productivity and yield. Different techniques (microscopy, serology, biochemical, physiological, molecular tools and culture propagation) are currently used to detect and identify bacterial pathogens. Detection and identification are critical steps for the appropriate application of phytosanitary measures. The "harmonization of phytosanitary regulations and all other areas of official plant protection action" mean the good practices for plant protection and plant material certification. The prevention of diseases progression and spread by early detection are a valuable strategy for proper pest management and disease control. For this purpose, innovative methods aim achieving results within a shorter time and higher performance, to provide rapidly, accurately and reliably diagnosis. In this review, we focus on the techniques for plant bacterial diagnosis and on the regulations for harmonizing plant protection issue.

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Section: Bacterial Pathogen Detection and Identificationmentioning

confidence: 99%

The diagnosis of plant pathogenic bacteria a state of art

2018

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“…Plant health regulation is built upon a base of risk assessment of known pests or pathogens (MacLeod 2015). Within this established system even simple changes in nomenclature and/or taxonomy of known pests can have major ramifications (Rossi et al 2014). The key issue with technologies such as NGS is that new putative pathogens (in particular viruses) can readily be identified on the basis of their genome, but that this identification is supported by little or no biological information.…”

Section: Assessing Risks From Newly Identified Pathogensmentioning

confidence: 99%

The role and challenges of new diagnostic technology in plant biosecurity

Mumford¹,

Macarthur²,

Boonham³

2015

Food Sec.

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Pest and pathogens pose a major threat to food security and the natural environment, and these threats are moving around the globe. Trade, travel and transport have major roles to play in this and thus to improve plant biosecurity, we need enhanced phytosanitary inspection systems. In order to achieve this, there is a role for the more effective use of diagnostic technology, such as field-based testing or the use of next generation sequencing technology. In this review we examine the opportunities and challenges posed by using new technology within a plant biosecurity context, but in contrast to previous reviews, here we focus on practical challenges associated with deployment and routine use, rather than specific technical issues. These key challenges include the need to accelerate the development and deployment of new technologies into the field, the accelerated discovery of new pathogens and the need for new risk assessment approaches, and improvements to our understanding of how best to deploy and use new diagnostic tools for maximum impact. Throughout we focus on how interdisciplinary approaches are important to help us improve our understanding and achieve our goals.

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“…inoxydabilis) infects various Asteraceae genera including Tanacetum, Zinnia and Matricaria (Boerema et al 2004;van der Aa et al 1990). Both pathogens are important quarantine organisms in many European countries (EPPO 2015), however, due to historical confusion in their taxonomy (Vaghefi et al 2012;Walker and Baker 1983), the reported host range and geographical distribution should be treated with caution (Rossi et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Differentiation of S. inoxydabilis and S. tanaceti based on morphology is also problematic and requires a high degree of expertise (Vaghefi et al 2012). The need for a reliable molecular diagnostic assay for identification and differentiation of the three Stagonosporopsis species associated with ray blight of Asteraceae has been emphasised in several studies (EFSA PLH Panel 2013; Rossi et al 2014;Vaghefi et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex PCR diagnostic assay for the detection of Stagonosporopsis species associated with ray blight of Asteraceae

et al. 2016

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Ray blight, a destructive disease of Asteraceae worldwide, is caused by three morphologically similar but phylogenetically distinct species; Stagonosporopsis chrysanthemi, S. inoxydabilis and S. tanaceti. Stagonosporopsis chrysanthemi has been reported as a specific pathogen of chrysanthemum while S. inoxydabilis has been found associated with various Asteraceae. Stagonosporopsis tanaceti has only been reported in Australia, causing substantial crop loss on pyrethrum. All three species were shown to infect and cause disease on in vitro grown pyrethrum plants, hence, S. chrysanthemi and S. inoxydabilis may pose a significant biosecurity threat to the Australian pyrethrum industry. All these Stagonosporopsis species are also Level 2 quarantine pathogens in Europe.Hence, rapid and accurate detection and differentiation of these species is a priority for ray blight management in Australia and in Europe. Accordingly, three species-specific PCR-based assays, targeted to the intergenic spacer of the nuclear ribosomal DNA, were developed. The specificity of each assay was confirmed against 21 Stagonosporopsis spp. as well as 14 pathogenic and saprophytic fungal species commonly found in association with pyrethrum in Australia. The primers were highly sensitive and specific to the target species, detecting down to 4 fg of genomic DNA. These primers were further used in a multiplex PCR to differentiate the presence of the three Stagonosporopsis spp. based on variable sized amplicons in a single reaction.

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Diagnosis of Plant Pathogens and Implications for Plant Quarantine: A Risk Assessment Perspective

Cited by 5 publications

References 89 publications

The diagnosis of plant pathogenic bacteria a state of art

The diagnosis of plant pathogenic bacteria a state of art

The role and challenges of new diagnostic technology in plant biosecurity

Development of a multiplex PCR diagnostic assay for the detection of Stagonosporopsis species associated with ray blight of Asteraceae

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