Diagnosis of Small Non–cleaved Cell Lymphoma by Fine Needle Aspiration Utilizing Cytomorphologic Features Combined with Cytogenetic Analysis

Schmitz, Laura; Beneke, Janet; Kubic, Virginia

doi:10.1159/000332700

Cited by 15 publications

(18 citation statements)

References 3 publications

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“…Cytomorphologically, a monomorphic lymphoid cell population with the presence of nucleoli and mitotic activity was the major clue to the diagnosis of NHL in these cases. This observation was in agreement with previous studies, 2,16,18,19 with a 100% cytohistological correlation in our study. We did not observe higher grades of monomorphic lymphoid cell aggregates or an increased number of mast cells in intermediate-grade NHL.…”

Section: Discussionsupporting

confidence: 95%

Fine‐needle aspiration biopsy in diagnosis of follicular lymphoma: Cytomorphologic and immunohistochemical analysis

Saikia

Dey

Saikia

et al. 2002

Diagnostic Cytopathology

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Follicular lymphoma is a relatively uncommon type of non-Hodgkin's lymphoma (NHL) thought to derive from follicular center cells. There are limitations to fine-needle aspiration in the diagnosis of follicular NHL, and very few studies are available on it. We describe here the cytomorphologic features of eight biopsy-proven cases of follicular NHLs and five biopsy-proven cases of intermediate-grade NHLs. Five cases of reactive lymphoid hyperplasia (RLH) diagnosed on cytology were also studied cytomorphologically. Image morphometry was done in follicular and intermediate-grade NHLs, immunocytochemistry was done in follicular and intermediate-grade NHLs, and immunocytochemistry for bcl-2 expression was studied in five cases of follicular NHLs. The aspirate smears of follicular NHL showed the presence of higher grades, many to abundant (++ to +++) monomorphic lymphoid aggregates, with an increased number of mast cells. Lymphohistiocytic tangles and tingeable body macrophages were not observed in any of the cases. Cytomorphologically, all the cases of intermediate-grade NHL showed a predominantly monomorphic population of lymphoid cells with absent or few grades (+) of lymphoid aggregates. Image morphometric data analysis of each cell parameter for follicular and intermediate-grade NHLs were found to be statistically insignificant (P > 0.05) and hence of little help in diagnosis of follicular vs. low-grade NHLs. Immunocytochemistry for bcl-2 expression in follicular NHL showed variable results, ranging from 0-90% (expressed as bcl-2 index). Certain characteristic cytomorphological features such as the presence of monomorphic lymphoid cell aggregates and an increased number of mast cells are soft indicators for the diagnosis of follicular NHL on aspiration smears. Immunocytochemistry for bcl-2 expression may be of help in cases with clinical and cytological suspicion of lymphoma. Image morphometry has a small role in this regard.

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Section: Discussionsupporting

confidence: 95%

Fine‐needle aspiration biopsy in diagnosis of follicular lymphoma: Cytomorphologic and immunohistochemical analysis

Saikia

Dey

Saikia

et al. 2002

Diagnostic Cytopathology

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“…Cytogenetic analysis with proof of the [t (8;14)] translocation may help to solve this problem. 33 Another problem in our study was the differentiation of malignant lymphoma from reactive lymphoid hyperplasia. In 3 cases reactive lymphoid hyperplasia was erroneously diagnosed as malignant lymphoma.…”

Section: Discussionmentioning

confidence: 95%

Fine Needle Aspiration Cytology and Flow Cytometry in the Diagnosis and Subclassification of Non-Hodgkin’s Lymphoma Based on the World Health Organization Classification

Bangerter¹,

Brudler²,

Heinrich³

et al. 2007

Acta Cytologica

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“…123 Detailed cytomorphologic and cytomorphometric parameters such as cell types, cell size, nuclear diameters, or mitotic indices can be studied in cytologic preparations of NHL and HD to supplement the subtyping of these entities. 103,124 Special ancillary techniques such as cytochemistry, 125 DNA flow cytometry, 126 immunocytochemistry, [127][128][129] cytogenetic analysis, 130 and Southern blot hybridization 131,132 are now performed on lymph node aspirates to diagnose lymphomas vs. other malignancies, and to decide the functional character of lymphomas and their clonal nature. Combining FNA and flow cytometric immunophenotyping (FCI) techniques has resulted in a rapid and accurate method for evaluating nodal and extranodal sites suspicious for malignant lymphoma, either by in vivo FNA 133 or by ex vivo FNA.…”

Section: Discussionmentioning

confidence: 99%

Value and limitations of fine-needle aspiration cytology in diagnosis and classification of lymphomas: A review

Das¹

1999

Diagn. Cytopathol.

104

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Diagnosis of Small Non–cleaved Cell Lymphoma by Fine Needle Aspiration Utilizing Cytomorphologic Features Combined with Cytogenetic Analysis

Cited by 15 publications

References 3 publications

Fine‐needle aspiration biopsy in diagnosis of follicular lymphoma: Cytomorphologic and immunohistochemical analysis

Fine‐needle aspiration biopsy in diagnosis of follicular lymphoma: Cytomorphologic and immunohistochemical analysis

Fine Needle Aspiration Cytology and Flow Cytometry in the Diagnosis and Subclassification of Non-Hodgkin’s Lymphoma Based on the World Health Organization Classification

Value and limitations of fine-needle aspiration cytology in diagnosis and classification of lymphomas: A review

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