Diagnosis of toenail onychomycosis by an immunochromatographic dermatophytes test strip

Mareschal, Adrien; Scherer, Émeline; Lihoreau, Thomas; Bellanger, Anne‐Pauline; Millon, Laurence; Aubin, F.

doi:10.1111/jdv.17128

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Testing can be performed directly in-office with both nail and skin samples. Currently, the presence of dermatophytes can be detected with good specificity using these existing flow devices, but results are not species-specific; cross-reaction has also been noted to a low degree with some non-dermatophyte species [ 40 , 41 , 42 , 43 , 44 ]. These tests are useful to provide indication of a likely dermatophyte infection, but further identification is required to select an optimum therapy.…”

Section: Organism Identification Methodsmentioning

confidence: 99%

Diagnosing Onychomycosis: What’s New?

Gupta

Hall

Cooper

et al. 2022

JoF

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An overview of the long-established methods of diagnosing onychomycosis (potassium hydroxide testing, fungal culture, and histopathological examination) is provided followed by an outline of other diagnostic methods currently in use or under development. These methods generally use one of two diagnostic techniques: visual identification of infection (fungal elements or onychomycosis signs) or organism identification (typing of fungal genus/species). Visual diagnosis (dermoscopy, optical coherence tomography, confocal microscopy, UV fluorescence excitation) provides clinical evidence of infection, but may be limited by lack of organism information when treatment decisions are needed. The organism identification methods (lateral flow techniques, polymerase chain reaction, MALDI-TOF mass spectroscopy and Raman spectroscopy) seek to provide faster and more reliable identification than standard fungal culture methods. Additionally, artificial intelligence methods are being applied to assist with visual identification, with good success. Despite being considered the ‘gold standard’ for diagnosis, clinicians are generally well aware that the established methods have many limitations for diagnosis. The new techniques seek to augment established methods, but also have advantages and disadvantages relative to their diagnostic use. It remains to be seen which of the newer methods will become more widely used for diagnosis of onychomycosis. Clinicians need to be aware of the limitations of diagnostic utility calculations as well, and look beyond the numbers to assess which techniques will provide the best options for patient assessment and management.

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Section: Organism Identification Methodsmentioning

confidence: 99%

Diagnosing Onychomycosis: What’s New?

Gupta

Hall

Cooper

et al. 2022

JoF

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Development, preparation, and evaluation of a novel dotted lateral flow immunochromatographic kit for rapid diagnosis of dermatophytosis

Aboul-Ella

Sayed

Abo-Elyazeed

2023

Sci Rep

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Dermatophytosis is a widely spread contagious zoonotic disease, affecting both man (tinea) and animals (ringworm). This disease is caused by a group of closely related keratinophilic fungi known collectively as the dermatophytes group. Although the wide distribution of dermatophytosis cases throughout the whole world and its adverse clinical effect on human health, economical effect on productive animals, and pet animal welfare, there is no rapid accurate diagnostic tool for such disease. The current conducted study tries to accomplish the difficult equation by achieving an accurate, sensitive, specific, user-friendly, rapid, robust, device-less, deliverable to end-users, and economic cost for the development and production of diagnostic kits. Through the development of a rapid diagnostic kit based on immunochromatographic assay with three major affordable reproducible production stages; preliminary stage, developmental and standardization stage, and evaluation stage. Obtaining dermatophytes-specific polyclonal antibodies against criteria-based selected dermatophytes strains associating proper gold nanoparticle preparation, characterization, and conjugation, with proper loading of the different bio-reactants on the efficiently laminated and fabricated lateral flow strips were the main challenge and control points through the whole process. Also, as a result of examining 100 animal samples using the new kit, the κ coefficients of the kit with the direct microscopy while the kit with the culture were 0.44 and 0.76, respectively. Therefore, the newly designated and developed kit showed a very promising competitive diagnostic result within 5–7 min through easy-to-be-performed three steps.

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Strategies to improve the diagnosis and clinical treatment of dermatophyte infections

Durdu

İlkit

2022

Expert Review of Anti-infective Therapy

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Diagnosis of toenail onychomycosis by an immunochromatographic dermatophytes test strip

Abstract: The realization of this project could not have been possible without the support provided by Fondazione Beretta to Dr. Moro for his training period at the Instituto Valenciano de Oncolog ıa.

Cited by 3 publications

References 12 publications

Diagnosing Onychomycosis: What’s New?

Diagnosing Onychomycosis: What’s New?

Development, preparation, and evaluation of a novel dotted lateral flow immunochromatographic kit for rapid diagnosis of dermatophytosis

Strategies to improve the diagnosis and clinical treatment of dermatophyte infections

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