Diagnostic Accuracy of Class 1 Integron PCR Method in Detection of Antibiotic Resistance in
            <i>Salmonella</i>
            Isolates from Swine Production Systems

Rao, Sangeeta; Maddox, Carol W.; Hoien-Dalen, Patricia S.; Lanka, Sara; Weigel, Ronald M.

doi:10.1128/jcm.01597-07

Cited by 18 publications

(21 citation statements)

References 27 publications

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“…A strong association between the presence of gene cassettes and resistance to specific antibiotics was also confirmed by earlier studies (Chang et al. 2000; Roe and Pillai 2003; Rao et al. 2008).…”

Section: Discussionsupporting

confidence: 80%

“…All isolates carrying the dfrA gene were resistant to SXT. A strong association between the presence of gene cassettes and resistance to specific antibiotics was also confirmed by earlier studies (Chang et al 2000;Roe and Pillai 2003;Rao et al 2008). However, in some isolates containing gene cassettes, the corresponding antibiotic resistance phenotype was not always present.…”

Section: Class I Integron and Upec In Lebanonsupporting

confidence: 73%

See 1 more Smart Citation

Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

El‐Najjar¹,

Farah²,

Hashwa³

et al. 2010

Letters in Applied Microbiology

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Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17‐aadA5, dfrA1‐aadA1, dfrA12‐orf5‐aadA2 and blaOXA‐30‐aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. Conclusions: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. Significance and Impact of the Study: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron‐associated antibiotic resistance genes in UPEC.

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Section: Discussionsupporting

confidence: 80%

Section: Class I Integron and Upec In Lebanonsupporting

confidence: 73%

Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

El‐Najjar¹,

Farah²,

Hashwa³

et al. 2010

Letters in Applied Microbiology

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“…The dhfr AXVII- aadA V gene cassette was present in the variable region of 12 integron-positive isolates, where the location of aadA V was close to the 3′CS and dhfr AXVII to the 5′CS region. Strong association between the presence of gene cassettes and resistance to specific antibiotics have been demonstrated in several studies (Chang et al, 2000 ; Roe and Pillai, 2003 ; Rao et al, 2008 ). On the contrary, in our study certain isolates were found to carry integron gene cassettes but the corresponding antibiotic resistance phenotype was absent.…”

Section: Discussionmentioning

confidence: 88%

Isolation and Genetic Analysis of Multidrug Resistant Bacteria from Diabetic Foot Ulcers

Shahi

Kumar

2016

Front. Microbiol.

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Severe diabetic foot ulcers (DFUs) patients visiting Sir Sunderlal Hospital, Banaras Hindu University, Varanasi, were selected for this study. Bacteria were isolated from swab and deep tissue of 42 patients, for examining their prevalence and antibiotic sensitivity. DFUs of majority of the patients were found infected with Enterococcus spp. (47.61%), Escherichia coli (35.71%), Staphylococcus spp. (33.33%), Alcaligenes spp. (30.95%), Pseudomonas spp. (30.95%), and Stenotrophomonas spp. (30.95%). Antibiotic susceptibility assay of 142 bacteria with 16 antibiotics belonging to eight classes showed the presence of 38 (26.76%) isolates with multidrug resistance (MDR) phenotypes. MDR character appeared to be governed by integrons as class 1 integrons were detected in 26 (68.42%) isolates. Altogether six different arrays of genes (aadA1, aadB, aadAV, dhfrV, dhfrXII, and dhfrXVII) were found within class 1 integron. Gene cassette dhfrAXVII-aadAV (1.6 kb) was present in 12 (3 Gram positive and 9 Gram negative) isolates and was conserved across all the isolates as evident from RFLP analysis. In addition to the presence of class 1 integron, six β-lactamase resistance encoding genes namely blaTEM, blaSHV, blaOXA, blaCTX−M−gp1, blaCTX−M−gp2, and blaCTX−M−gp9 and two methicillin resistance genes namely mecA and femA and vancomycin resistance encoding genes (vanA and vanB) were identified in different isolates. Majority of the MDR isolates were positive for blaTEM (89.47%), blaOXA (52.63%), and blaCTX−M−gp1 (34.21%). To our knowledge, this is the first report of molecular characterization of antibiotic resistance in bacteria isolated from DFUs from North India. In conclusion, findings of this study suggest that class-1 integrons and β-lactamase genes contributed to the MDR in above bacteria.

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“…PCR analysis for the florfenicol / chloramphenicol resistance gene floR was performed using the method of Khan et al (2000). The presence of class 1 integrons was determined by the Int-PCR assay (Rao et al 2008). Phage typing for the DT104 phage was performed on this strain by PCR using the method of Pritchett et al (2000).…”

Section: Methodsmentioning

confidence: 99%

Elucidation of antimicrobial susceptibility profiles and genotyping of Salmonella enterica isolates from clinical cases of salmonellosis in New Mexico in 2008

Smith

George²,

Cadle

et al. 2009

World J Microbiol Biotechnol

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In this study, we investigated the antimicrobial susceptibility profiles and the distribution of some well known genetic determinants of virulence in clinical isolates of Salmonella enterica from New Mexico. The minimum inhibitory concentrations (MICs) for various antimicrobials were determined by using the E-test strip method according to CLSI guidelines. Virulence genotyping was performed by polymerase chain reaction (PCR) using primers specific for known virulence genes of Salmonella enterica. Of 15 isolates belonging to 11 different serovars analyzed, one isolate of Salmonella Typhimurium was resistant to multiple drugs namely ampicillin, amoxicillin / clavulanic acid, chloramphenicol and tetracycline, that also harbored class 1 intergron, bla TEM encoding genes for β-lactamase, chloramphenicol acetyl transferase (cat1), plus floR, tet(C) and tet(G). This strain was phage typed as DT104. PCR analysis revealed the presence of invA, hilA, stn, agfA and spvR virulence genes in all the isolates tested. The plasmid-borne pefA gene was absent in 11 isolates, while 5 isolates lacked sopE. One isolate belonging to serogroup E4 (Salmonella Sombre) was devoid of multiple virulence genes pefA, iroB, shdA and sopE. These results demonstrate that clinical Salmonella serotypes from New Mexico used here are predominantly sensitive to multiple antimicrobial agents, but vary in their virulence genotypes. Information on antimicrobial sensitivity and virulence genotypes will help in understanding the evolution and spread of epidemic strains of Salmonella enterica in the region of study.

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Diagnostic Accuracy of Class 1 Integron PCR Method in Detection of Antibiotic Resistance in Salmonella Isolates from Swine Production Systems

Cited by 18 publications

References 27 publications

Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

Isolation and Genetic Analysis of Multidrug Resistant Bacteria from Diabetic Foot Ulcers

Elucidation of antimicrobial susceptibility profiles and genotyping of Salmonella enterica isolates from clinical cases of salmonellosis in New Mexico in 2008

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