“…Despite being known as the gold standard for the detection of IgG concentrations (Fleenor and Stott, 1981), the radial immunodiffusion (RID) assay has not found common use on the field, given that it is expensive, produces results only after 24 h, is influenced by temperature alterations during incubation, and is required to be performed by experienced staff. Thus, alternative methods have been investigated for the assessment of passive immunity status (Pekcan et al, 2013;Deelen et al, 2014;Elsohaby et al, 2014;Hogan et al, 2015;Drikic et al, 2018;Topal et al, 2018;Akköse et al, 2022a). Digital and optical Brix refractometers are efficient, lowcost and practical devices that can be used on the farm to detect ITPI.…”