The acoustic voice quality index (AVQI) is a multiparametric tool based on six acoustic measurements to quantify overall voice quality in an objective manner, with the smoothed version of the cepstral peak prominence (CPPS) as its main contributor. In the last decade, many studies demonstrated its robust diagnostic accuracy and high sensitivity to voice changes across voice therapy in different languages. The aim of the present study was to provide information regarding AVQI’s and CPPS’s performance in normophonic non-treatment-seeking subjects, since these data are still scarce; concatenated voice samples, consisting of sustained vowel phonation and continuous speech, from 123 subjects (72 females, 51 males; between 20 and 60 years old) without vocally relevant complaints were evaluated by three raters and run in AVQI v.02.06. According to this auditory-perceptual evaluation, two cohorts were set up (normophonia versus slight perceived dysphonia). First, gender effects were investigated. Secondly, between-cohort differences in AVQI and CPPS were investigated. Thirdly, with the number of judges giving G = 1 to partition three sub-levels of slight hoarseness as an independent factor, differences in AVQI and CPPS across these sub-levels were investigated; for AVQI, no significant gender effect was found, whereas, for CPPS, significant trends were observed. For both AVQI and CPPS, no significant differences were found between normophonic and slightly dysphonic subjects. For AVQI, however, this difference did approach significance; these findings emphasize the need for a normative study with a greater sample size and subsequently greater statistical power to detect possible significant effects and differences.