Diagnostic accuracy of genetic markers for identification of the <i>Lr46/Yr29</i> “slow rusting” locus in wheat (<i>Triticum aestivum</i> L.)

Bobrowska, Roksana; Noweiska, Aleksandra; Spychała, Julia; Tomkowiak, Agnieszka; Kwiatek, Michał

doi:10.1515/bmc-2022-0002

Cited by 4 publications

(4 citation statements)

References 41 publications

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“…Stripe rust resistance gene Yr69 has also been found to be bordered proximally by Xbarc124 (Hou et al 2016). Bobrowska et al (2022) found that Xwmc44 is the appropriate marker for detection of Lr46/Yr29 gene's resistance allele. Similarly, Yr59, an adult plant resistance gene was positioned on the long arm of chromosome 7B, and was tagged by Xbarc32 (Zhou et al 2014).…”

Section: Resultsmentioning

confidence: 99%

“…This gene provides partial broad spectrum resistance against leaf rust and is an ideal target for stacking with other disease resistance genes (Singla et al 2017). Bobrowska et al (2022) has checked the diagnostic accuracy of genetic markers for identification of slow rusting locus in wheat. Functional markers identified in the present study can also be validated using similar course of study.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of wheat (Triticum aestivum) genotypes for multiple fungal resistance using functional markers

et al. 2023

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Wheat (Triticum aestivum L.) encounters 15–20% yield loss due to fungal diseases. A study was carried out to analyse the allelic variations in functional genes associated with multiple fungal disease resistance, viz. rusts, smuts and powdery mildew in 58 contrasting wheat genotypes. The experiments were conducted at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana during 2020–21. A set of 29 simple sequence repeat (SSR) markers was selected for screening, out of which 24 markers showed amplifications (82.7%) and 23 showed polymorphism (95.83%) with a total of 46 alleles. Alleles per locus varied from 1 to 3 with a mean of 1.96 alleles per locus. At a similarity coefficient of 0.66, dendrogram grouped all the genotypes into 2 major clusters. Two and three dimensional plots also confirmed the distribution. Results showed that genotypes PBW 725 and WH 1268 were found to be most diverse at a similarity coefficient of 77%. SSR polymorphism rates were analysed using polymorphism information content, expected heterozygosity, marker index, discriminating power and resolving power values, where first two ranged from 0.03–0.65, and later three ranged from 0.03–1.94, 0.03–0.66 and 0.03–2.00, respectively. Based on these results, 8 proficient markers, viz. Barc232, Swm271, Xbarc124, Xbarc32, Xwmc44, Xgwm296, Gpw5029 and Xwmc557 are suggested for Indian wheat fungal disease resistance profiling. Among these, first two markers (Barc232 and Swm271) were detected in most (57) of the genotypes which are associated with ut6 and Lr75 genes, providing resistance to loose smut and leaf rust, respectively. This study can further help in gene pyramiding for producing multiple disease resistant genotypes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Characterization of wheat (Triticum aestivum) genotypes for multiple fungal resistance using functional markers

et al. 2023

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“…The molecular markers used successfully identified selected Lr resistance genes in the hybrid forms tested ( Table 2 ). It has been reported in the literature that STS marker csLV34 is highly reliable for Lr34 [ 25 , 33 , 34 ], and csLV46G22 is a highly reliable and close diagnostic marker for the Lr46 gene [ 22 , 27 , 35 ]. In the case of the presence of Lr34 , we obtained a product of 150 bp.…”

Section: Resultsmentioning

confidence: 99%

“…PCR was performed with FastGene Optima HotStart ReadyMix reagents (NIPPON Genetics Europe GmbH, Düren, Germany), using a Labcycler thermal cycler (SensoQuest GmbH, Göttingen, Germany). The composition of the reaction mixture is given in our previous work [ 27 ]. The PCR consisted of initial denaturation at 94 °C for 3 min, followed by 35 cycles (denaturation, 94 °C for 30 s; primer annealing, 60 °C for 30 s; elongation, 72 °C for 90 s), followed by the final extension for 10 min at 72 °C and storage at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Diversity of Expression Patterns of Lr34, Lr67, and Candidate Genes towards Lr46 with Analysis of Associated miRNAs in Common Wheat Hybrids in Response to Puccinia triticina Fungus

Spychała,

Tomkowiak,

Noweiska

et al. 2024

CIMB

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Leaf rust caused by Puccinia triticina (Pt) is one of the most dangerous diseases causing significant losses in common wheat crops. In adult plants resistant to rust, a horizontal adult plant resistance (APR) type is observed, which protects the plant against multiple pathogen races and is distinguished by greater persistence under production conditions. Crucial pleiotropic slow-rust genes such as Lr34, Lr46, Lr67, and Lr68, in combination with other genes of lesser influence, continue to increase durable resistance to rust diseases. Based on our previous results, we selected four candidate genes for Lr46 out of ten candidates and analysed them for expression before and after inoculation by P. triticina. As part of our study, we also investigated the expression patterns of miRNA molecules complementary to Lr34 and the candidate genes. The aim of the study was to analyse the expression profiles of candidate genes for the Lr46 gene and the Lr34 and Lr67 genes responsible for the differential leaf-rust resistance of hybrid forms of the F1 generation resulting from crosses between the Glenlea cultivar and cultivars from Polish breeding companies. In addition, the expression of five miRNAs (tae-miR9653b, tae-miR5384-3p, tae-miR9780, tae-miR9775 and tae-miR164), complementary to Lr34, and selected candidate genes were analysed using stem-loop RT-PCR and ddPCR. Biotic stress was induced in adult plants by inoculation with Pt fungal spores, under controlled conditions. Plant material was collected before and 6, 12, 24, and 48 h after inoculation (hpi). Differences in expression patterns of Lr34, Lr67, and candidate genes (for Lr46) were analysed by qRT-PCR and showed that gene expression changed at the analysed time points. Identification of molecular markers coupled to the Lr genes studied was also carried out to confirm the presence of these genes in wheat hybrids. qRT-PCR was used to examine the expression levels of the resistance genes. The highest expression of Lr46/Yr29 genes (Lr46-Glu2, Lr46-RLK1, Lr46-RLK2, and Lr46-RLK3) occurred at 12 and 24 hpi, and such expression profiles were obtained for only one candidate gene among the four genes analysed (Lr46-Glu2), indicating that it may be involved in resistance mechanisms of response to Pt infection.

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A Combination of Resistance Genes Confers High and Durable Resistance Against Stripe Rust in Wheat Cultivar Lantian 26

Wang,

Liu

et al. 2024

Plant Disease

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Lantian 26, a leading elite winter wheat cultivar in Gansu Province since its release in 2010, exhibits high resistance or immunization to stripe rust in adult-plant stage under a high disease pressure in Longnan (southeastern Gansu). Identifying the resistance genes in Lantian 26 could provide a basis for enhanced durability and high levels of resistance in wheat cultivars. Here, a segregating population was developed from a cross between a highly susceptible wheat cv. Mingxian 169 and the highly stripe rust-resistant cv. Lantian 26. The F2 and F2:3 progenies of the cross were inoculated with multiple prevalent virulent races of stripe rust for adult plant-stage resistance evaluation in two different environments. Exon sequence alignment analysis revealed that a stripe rust resistance gene on the 718.4-721.2 Mb region of chromosome 7BL, tentatively named as YrLT26, and a co-segregation STS marker GY17 was developed and validated using the F2:3 population and 103 wheat cultivars. The other two resistance genes, Yr9 and Yr30, were also identified in Lantian 26 using molecular markers. Therefore, the key to high and durable resistance to stripe rust at adult stage is the combination of Yr9, Yr30 and YrLT26 genes in Lantian 26. This could be a considerable strategy for improving the wheat cultivars with effective and durable resistance in the high-pressure region for stripe rust.

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Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.)

Cited by 4 publications

References 41 publications

Characterization of wheat (Triticum aestivum) genotypes for multiple fungal resistance using functional markers

Characterization of wheat (Triticum aestivum) genotypes for multiple fungal resistance using functional markers

Diversity of Expression Patterns of Lr34, Lr67, and Candidate Genes towards Lr46 with Analysis of Associated miRNAs in Common Wheat Hybrids in Response to Puccinia triticina Fungus

A Combination of Resistance Genes Confers High and Durable Resistance Against Stripe Rust in Wheat Cultivar Lantian 26

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