Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma

Wang, Dong; Li, Baosheng; Wang, Juan; Song, Yipeng; Zhang, Zicheng; Fu, Cuiping; Zhang, Peiliang

doi:10.3892/or.2015.4499

Cited by 24 publications

(16 citation statements)

References 49 publications

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“…miR-7 has been considered as a double-edged sword where it may act either as an oncomiR or as a TS-miR in different tumor types through its participation in distinct pathways and regulation of discrete target genes in different cell types. 12,[30][31][32][33][34][35][36][37] This study revealed that serum miR-7 RQ values were significantly lower in the HCC group compared to both CLD and healthy control groups (p < 0.01). In fact, most studies describing the role of miR-7 in HCC have been in vitro experiments with the exception of the study done by Fang et al 14 who analyzed the expression of miR-7 using qRT-PCR in 10 pairs of clinical HCC tissue samples and reported repression of miR-7 expression in 7 of 10 HCC cases compared to paired normal tissues.…”

Section: Discussionmentioning

confidence: 79%

Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma

et al. 2017

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Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). IntroductionHepatocellular carcinoma (HCC) represents the fifth most common cancer and the third most leading cause of cancer-related deaths worldwide. 1 High morbidity observed in HCC is majorly attributed to the lack of early detection markers and poor prognosis. Hence, exploration of novel frontiers in HCC diagnosis and therapeutics remains to be high priority research areas. 2,3 HCC represents a public health problem in Egypt. It constitutes 70.48% of all liver tumors among Egyptians, representing the second most common malignancy after bladder cancer in men and breast cancer in women and the second most common cause of death in men. 4,5 Alpha-fetoprotein (AFP) is the most widely used tumor biomarker for HCC diagnosis. However, it has low sensitivity and specificity. This highlights the need for other methods that would be minimally invasive, simple, and reliable. 6 MicroRNAs (miRNAs) are a class of short non-coding RNAs, which play a central role in sequence specific posttranscriptional gene attenuation. 7 They are involved in various fundamental cellular processes as well as carcinogenesis. Moreover, miRNAs are highly stable in serum due to their resistance to RNase, extreme pH, and temperature. Therefore, they have been identified as candidate AbstractThere is an obvious need to diagnose hepatocellular carcinoma using novel non-invasive and sensitive biomarkers. In this regard, the aim of this study was to evaluate and correlate both relative quantification of microRNA-7 using quantitative real time polymerase chain reaction and quantitative analysis of selenoprotein P using enzyme-linked immunosorbent assay in sera of hepatocellular carcinoma patients, chronic liver disease patients, as well as normal healthy subjects in order to establish a new diagnostic biomarker with a valid non-invasive technique. In addition, this study aimed to investigate whether changes in selenium supply affect microRNA-7 expression and selenoprotein P levels in human hepatocarcinoma cell line (HepG2). The results showed a highly significant decrease in serum microRNA-7 relative quantification values and selenoprotein P levels in malignant group in comparison with benign and control groups. The best cutoff for serum microRNA-7 and selenoprotein P to discriminate hepatocellular carcinoma group from benign and control groups was 0.06 and 4.30 mg/L, respectively. Furthermore, this study showed that changes in selenium supply to HepG2 cell line can alter the microRNA-7 profile and are paralleled by changes in the concentration of its target protein (selenoprotein P). Hence, serum microRNA-7 ...

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Section: Discussionmentioning

confidence: 79%

Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma

et al. 2017

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“…While this manuscript was in preparation, Hanniford and coworkers reported that reduced miR-7-5p expression was associated with melanoma recurrence and reduced invasion in vitro [47]. Another recent report suggested that serum miR-7-5p levels might be a useful biomarker for tumors of the esophagus [48]. Certainly, additional studies are required to more clearly define the prognostic significance of miR-7-5p expression in large cohorts of primary and metastatic melanoma.…”

Section: Discussionmentioning

confidence: 99%

microRNA-7-5p inhibits melanoma cell proliferation and metastasis by suppressing RelA/NF-κB

et al. 2016

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microRNA-7-5p (miR-7-5p) is a tumor suppressor in multiple cancer types and inhibits growth and invasion by suppressing expression and activity of the epidermal growth factor receptor (EGFR) signaling pathway. While melanoma is not typically EGFR-driven, expression of miR-7-5p is reduced in metastatic tumors compared to primary melanoma. Here, we investigated the biological and clinical significance of miR-7-5p in melanoma. We found that augmenting miR-7-5p expression in vitro markedly reduced tumor cell viability, colony formation and induced cell cycle arrest. Furthermore, ectopic expression of miR-7-5p reduced migration and invasion of melanoma cells in vitro and reduced metastasis in vivo. We used cDNA microarray analysis to identify a subset of putative miR-7-5p target genes associated with melanoma and metastasis. Of these, we confirmed nuclear factor kappa B (NF-κB) subunit RelA, as a novel direct target of miR-7-5p in melanoma cells, such that miR-7-5p suppresses NF-κB activity to decrease expression of canonical NF-κB target genes, including IL-1β, IL-6 and IL-8. Importantly, the effects of miR-7-5p on melanoma cell growth, cell cycle, migration and invasion were recapitulated by RelA knockdown. Finally, analysis of gene array datasets from multiple melanoma patient cohorts revealed an association between elevated RelA expression and poor survival, further emphasizing the clinical significance of RelA and its downstream signaling effectors. Taken together, our data show that miR-7-5p is a potent inhibitor of melanoma growth and metastasis, in part through its inactivation of RelA/NF-κB signaling. Furthermore, miR-7-5p replacement therapy could have a role in the treatment of this disease.

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“…Moreover, docetaxel inhibited cell proliferation via upregulation of miR-7 expression in NSCLC cells 18. Furthermore, mutations within the miR-7 promoter are associated with poor prognosis of lung cancer,10 and serum miR-7 levels are a predictive biomarker in esophageal squamous cell carcinoma 19. Interestingly, miR-7 was identified as an oncogene in renal cell carcinoma 20.…”

Section: Introductionmentioning

confidence: 99%

Curcumin exerts its antitumor activity through regulation of miR-7/Skp2/p21 in nasopharyngeal carcinoma cells

Feng¹,

Wang²,

Zhang³

et al. 2017

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Curcumin, a natural polyphenol compound, exhibits tumor suppressive activity in a wide spectrum of cancers, including nasopharyngeal carcinoma cells. However, the exact molecular mechanisms governing this tumor suppressive activity remain elusive. Multiple studies have revealed that miRNAs are critically involved in tumorigenesis, indicating that targeting miRNAs could be a therapeutic strategy for treating human cancer. In the current study, we set out to determine whether curcumin regulates miR-7 expression in nasopharyngeal carcinoma cells. We found that curcumin inhibited cell growth, induced apoptosis, retarded cell migration and invasion, and triggered cell cycle arrest in the human nasopharyngeal carcinoma cell lines CNE1 and CNE2. Importantly, we observed that curcumin upregulated the expression of miR-7 and subsequently inhibited Skp2, a direct miR-7 target. Our results identified that upregulation of miR-7 by curcumin could benefit nasopharyngeal carcinoma patients.

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Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma

Cited by 24 publications

References 49 publications

Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma

Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma

microRNA-7-5p inhibits melanoma cell proliferation and metastasis by suppressing RelA/NF-κB

Curcumin exerts its antitumor activity through regulation of miR-7/Skp2/p21 in nasopharyngeal carcinoma cells

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