Diagnostic and prognostic value of HABP2 as a novel biomarker for endometrial cancer

Abstract

Background: Endometrial cancer is the fifth most common malignant disorder in women, with its incidence increasing. A biomarker with diagnostic and prognostic value remains to be found. The HABP2 protein, or Factor VII-activating protease, encodes a hyaluronic acid-binding protein.Methods: Patient data including clinical characteristics and RNAseq information of HABP2 was obtained from The Cancer Genome Atlas (TCGA), and analyzed by R statistic packages. A total of 370 women with endometrial cancer were enrolled in the study. To study the diagnostic value of HABP2 in patients with endometrial cancer, receiver operating characteristic (ROC) curves were plotted by the pROC package. To study the prognostic value of HABP2 in patients with endometrial cancer, the survival package in R was used and the Cox model was established.Results: HABP2 expression was lower in endometrial cancer compared with normal endometrial tissues. HABP2 showed moderate diagnostic value for endometrial cancer, with HBP2 expression associated with vital status, histologic grade, and residual tumor. HABP2 was an independent prognostic factor, with low HABP2 expression indicating a better overall survival.Conclusions: HABP2 has diagnostic and prognostic value and maybe a novel biomarker for endometrial cancer.

Exploration of Potential Biomarkers and Immune Landscape for Hepatoblastoma: Evidence from Machine Learning Algorithm

“…The HB cell lines (SMMC-7721) and the normal human hepatic cell line (L02) were cultured in 10% FBS DMEM medium in an environment of 5% CO2 and 37°C [ 23 ]. Primer sequences are summarized in previous studies, including TRIB1 [ 24 ], MAT1A [ 25 ], SAA2-SAA4 [ 26 ], NAT2 [ 27 ], HABP2 [ 28 ], CYP2CB [ 29 ], APOF [ 30 ], and CFHR3 [ 31 ]. Detailed experimental procedures are described in our previous publications [ 32 , 33 ].…”

Role of Hyaluronic Acid in Selected Malignant Neoplasms in Women

“…In contrast, a study by Jiang et al [6] conducted on tissue specimens from 370 women with EC (analysis of the data from patients suffering from EC) detected increased expression of the HABP2 protein; however, decreased expression of this protein was associated with longer OS. An association of HABP2 with increased histological maturity (malignancy) of cancer and residual tumor after surgery has been reported [6]. These studies indicate the possible utility of changes in HABP1/2 protein expression as diagnostic markers or independent prognostic factors in EC.…”

“…High expression was simultaneously associated with increased disease stage, infiltration of lymphatic spaces, and metastasis, as well as shorter DFS and OS [59]. In contrast, a study by Jiang et al [6] conducted on tissue specimens from 370 women with EC (analysis of the data from patients suffering from EC) detected increased expression of the HABP2 protein; however, decreased expression of this protein was associated with longer OS. An association of HABP2 with increased histological maturity (malignancy) of cancer and residual tumor after surgery has been reported [6].…”

“…The biological activity of HA depends on attachment to hyaluronan binding proteins (HABPs) (Figure 2B). This group includes the proteins bound to the cell membrane (e.g., CD44, TLR2/4, HARE), as well as the proteins that are part of the extracellular matrix [5][6][7]. The main HA receptor is CD44 protein (cluster of differentiation 44), a single-chain trans-membrane glycoprotein (Figure 2B), which is also one of the most relevant markers of cancer stem cells (CSCs) in many types of cancer [8][9][10].…”

“…In the course of cancer development, HA affects, among other things, the migration, invasiveness, and resistance of cancer cells to the chemotherapeutic drugs applied [4,5]. HA is a factor involved in carcinogenesis [5,6,19] through its interaction with specific receptors and intracellular signal transduction. HA regulates the microenvironment of tumors, thus promoting their malignant phenotype.…”