Diagnostic application of lipidomics fingerprints to bladder carcinoma

Cruciani, Gabriele; Pellegrino, Roberto Maria; Veroli, Alessandra Di; Cataldi, Samuela; Marocco, Daniela; Costantini, Elisabetta; Sidoni, Angelo; Viola-Magni, Mariapia

doi:10.21037/tcr.2017.11.11

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, autotaxin–lysophosphatidic acid signaling has been implicated in ovarian cancer [ 16 , 17 , 18 ]. Neutral lipids such as triacylglycerols, diacylglycerols, and cholesteryl esters have also been reported to be altered in cancer [ 19 , 20 , 21 ]. These studies highlight the importance of in-depth lipidomic analysis in cancers for elucidation of potential molecular signatures of carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic Analysis of Archival Pathology Specimens Identifies Altered Lipid Signatures in Ovarian Clear Cell Carcinoma

et al. 2021

View full text Add to dashboard Cite

Cancer metabolism is associated with the enhanced lipogenesis required for rapid growth and proliferation. However, the magnitude of dysregulation of diverse lipid species still requires significant characterization, particularly in ovarian clear cell carcinoma (OCCC). Here, we have implemented a robust sample preparation workflow together with targeted LC-MS/MS to identify the lipidomic changes in formalin-fixed paraffin-embedded specimens from OCCC compared to tumor-free ovarian tissue. We quantitated 340 lipid species, representing 28 lipid classes. We observed differential regulation of diverse lipid species belonging to several glycerophospholipid classes and trihexosylceramide. A number of unsaturated lipid species were increased in OCCC, whereas saturated lipid species showed a decrease in OCCC compared to the controls. We also carried out total fatty acid analysis and observed an increase in the levels of several unsaturated fatty acids with a concomitant increase in the index of stearoyl-CoA desaturase (SCD) in OCCC. We confirmed the upregulation of SCD (the rate-limiting enzyme for the synthesis of monounsaturated fatty acids) by immunohistochemistry (IHC) assays. Hence, by carrying out a mass spectrometry analysis of archival tissue samples, we were able to provide insights into lipidomic alterations in OCCC.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipidomic Analysis of Archival Pathology Specimens Identifies Altered Lipid Signatures in Ovarian Clear Cell Carcinoma

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Two hundred and fifty lipids were analyzed and a significant difference was observed, in particular for 25 lipids that appear to be characteristic for the tumor patient. Their identification may offer the possibility of a marker that appears to have sensitivity and a specificity of around 100% (Figure 3) [147].…”

Section: Analysis Of Isolated Virtosomes From Voided Urinementioning

confidence: 99%

Bladder Cancer Markers and Recent Innovations

Viola-Magni¹,

Cataldi²,

Marocco³

2017

Bladder Cancer - Management of NMI and Muscle-Invasive Cancer

Self Cite

View full text Add to dashboard Cite

Bladder cancer (urothelial carcinoma) is the most common tumor of the urinary tract. It occurs more frequently among men about 65 years old on average. Two forms of the tumor are known: a non-muscle-invasive one and a muscle-invasive one. The latter turns out to be very aggressive with a survival of 5 years average. The non-muscle-invasive form frequently recurs (60-70%) and in 15% of cases, it progresses into the invasive form. The diagnosis is made mainly by cystoscopy and urine cytology. A high number of researches were dedicated in order to find a simple test using voided urine to frequently monitor possible tumor recurrence. During the last 10 years, many tests were proposed concerning either special proteins of which the most common are the bladder tumor antigen (BTA) and the nuclear matrix protein 22 (NMP22) or the presence of genetic mutations [most frequently, fibroblasts growth factor receptor 3 (FGFR3) and TP53], alteration of DNA methylation, chromatin structure and, more recently, the presence of specific micro-RNA. Recently the analysis of lipids present in voided urine showed a difference in fatty acids between healthy individuals and those affected by non-invasive forms. These markers appear to have a high specificity and sensitivity: a deepening of these results could lead to the development of a test that avoids invasive treatment and the cost of cystoscopy.

show abstract

“…These four major classes of lipids have been frequently investigated as potential tumor-related biomarkers in lipidomic research. , For example, the changes of LPE (18:1), PE (P-40:3), CE (18:2), and SM (22:0) in serum can distinguish lung cancer patients from healthy people . Variation of TG and CE in urine samples is also expected to be a diagnostic marker for bladder cancer …”

mentioning

confidence: 99%

“…6 Variation of TG and CE in urine samples is also expected to be a diagnostic marker for bladder cancer. 7 Among various body fluid samples for lipidomic research, the serum is the first choice because it contains rich lipid molecules and is widely used in clinical trials. 8 The major platform for serum lipidomic research is liquid chromatography−mass spectrometry (LC−MS), 9 which is able to detect high coverage of lipid molecules owing to the good separation ability of LC.…”

mentioning

confidence: 99%

Dual-Mechanism-Driven Strategy for High-Coverage Detection of Serum Lipids on a Novel SALDI-MS Target

Wang

Liu

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Serum lipid metabolites have been emerging as ideal biomarkers for disease diagnosis and prediction. In the current stage, nontargeted or targeted lipidomic research mainly relies on a liquid chromatography–mass spectrometry (LC–MS) platform, but future clinical applications need more robust and high-speed platforms. Surface-assisted laser desorption ionization mass spectrometry (SALDI-MS) has shown excellent advantages in the high-speed analysis of lipid metabolites. However, the platform in the positive ion mode is more inclined to target a certain class of lipids, leading to the low coverage of lipid detection and limiting its practical translation to clinical applications. Herein, we proposed a dual-mechanism-driven strategy for high-coverage detection of serum lipids on a novel SALDI-MS target, which is a composite nanostructure comprising vertical silicon nanowires (VSiNWs) decorated with AuNPs and polydopamine (VSiNW-Au-PDA). The performance of laser desorption and ionization on the target can be enhanced by charge-driven desorption coupled with thermal-driven desorption. Simultaneous detection of 236 serum lipids (S/N ≥ 5) including neutral and polar lipids can be achieved in the positive ion mode. Among these, 107 lipid peaks were successfully identified. When combined with VSiNW-Au-PDA and VSiNW chips, 479 lipid peaks can be detected in serum samples in positive and negative ion modes, respectively. Based on the platform, serum samples from 57 hepatocellular carcinoma (HCC) patients and 76 healthy controls were analyzed. After data mining, 14 lipids containing different lipid types (TAG, CE, PC) were selected as potential lipidomic biomarkers. With the assistance of an artificial neural network, a diagnostic model with a sensitivity of 92.7% and a specificity of 96% was constructed for HCC diagnosis.

show abstract

Diagnostic application of lipidomics fingerprints to bladder carcinoma

Cited by 4 publications

References 0 publications

Lipidomic Analysis of Archival Pathology Specimens Identifies Altered Lipid Signatures in Ovarian Clear Cell Carcinoma

Lipidomic Analysis of Archival Pathology Specimens Identifies Altered Lipid Signatures in Ovarian Clear Cell Carcinoma

Bladder Cancer Markers and Recent Innovations

Dual-Mechanism-Driven Strategy for High-Coverage Detection of Serum Lipids on a Novel SALDI-MS Target

Contact Info

Product

Resources

About