Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: implications for the monitoring of illegal hunting and wildlife trade

Silva-Neto, A. A.; Ferreira, P. B.; Torres, Rodrigo A.; Texeira, R. H. F.; Duarte, J. M. B.; Barbosa, Antônio C.; Vargas, Ricardo Cruz; García, José Eduardo

doi:10.1590/1519-6984.10814

Cited by 8 publications

(5 citation statements)

References 15 publications

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“…cyt b gene is one of the genes located in the mitochondrial genome that is widely used for molecular analyses. cyt b gene possesses fast and slow developments of codon positions which have conservative parts and ever-changing parts [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The phylogenetics of Leucocytozoon caulleryi infecting broiler chickens in endemic areas in Indonesia

Yuniarti

2017

Vet World

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Aim:The objective of this research was to determine the species and strains of Leucocytozoon caulleryi and study the phylogenetics of L. caulleryi of broiler chickens in endemic areas in Indonesia.Materials and Methods:Blood samples were collected from broiler chickens originated from endemic area in Indonesia, i.e., Pasuruan, Lamongan, Blitar, Lumajang, Boyolali, Purwokerto, and Banjarmasin in 2017. Collected blood was used for microscopic examination, sequencing using BLAST method to identify the nucleotide structure of cytochrome b (cyt b) gene that determines the species, and the phylogenetics analysis of L. caulleryi that infected broiler chickens in endemic areas in Indonesia, using Mega 5 software.Results:The results showed that Plasmodium sp. and L. caulleryi were infected broiler chickens in endemic areas in Indonesia. L. caulleryi in one area had very close phylogenetic relations with those in other areas. The genetic distance between L. caulleryi taxa from various endemic areas is very close (<5%).Conclusion:There is a very close phylogenetics among strains of L. caulleryi that infected broiler chickens in various endemic areas in Indonesia.

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Section: Introductionmentioning

confidence: 99%

The phylogenetics of Leucocytozoon caulleryi infecting broiler chickens in endemic areas in Indonesia

Yuniarti

2017

Vet World

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“…Using the Cyt b gene and direct PCR, a rare paka animal was identified in the illegal meat trade. The method based on a single-nucleotide substitution of the Cyt b gene proved effective and differentiated paka meat with 100% certainty [14]. The presented results demonstrate the usefulness of PCR analysis based on primers for the Cyt b gene in the authentication of meat and meat products in the laboratory.…”

Section: Discussionmentioning

confidence: 63%

“…These markers are not only reliable and sensitive but also relatively inexpensive. Using the SNP fragment of the D-loop gene of mitochondrial DNA effectively identified 10 wild African animal species that were affected by illegal hunting [14]. The cytochrome b (Cyt b) gene is widely used to identify vertebrate species.…”

Section: Introductionmentioning

confidence: 99%

Optimization of polymerase chain reaction for the identification of Roe deer, Saiga, and Siberian stag living in Kazakhstan

et al. 2022

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Background and Aim: One of the reasons for the decline in the number of wild species of artiodactyls is poaching and the illegal trading of animal products. Molecular genetic identification of animals from a biological sample effectively proves poaching cases and illegal trade of animal products. This study aimed to develop a polymerase chain reaction (PCR) test that allows for species identification of artiodactyl animals that are most often subject to poaching. Materials and Methods: Genomic DNA was extracted from meat and blood samples of animals killed by poachers using commercial kits. Three pairs of primers were designed and used to amplify the cytochrome b gene fragment of Roe deer, Saiga antelope, and Siberian stag. Results: The proposed protocol allows amplification of specific PCR products of 542 bp with Roe deer DNA, 587 bp with Saiga DNA, and 525 bp with Siberian stag DNA. Specificity analysis showed no cross activity with DNA from other animal species. The detection limit of PCR ranged from 15.6 pg to 1.9 pg of DNA in 25 μL of the reaction mixture. Conclusion: Sequencing the amplified products and subsequent comparison with the corresponding reference sequence showed a similarity ranging from 99.99% to 100%. The PCR based on the developed primers demonstrated high sensitivity and specificity when using DNA from homogeneous and heterogeneous animals.

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“…The reasons for the growth in hunting activity in tropical forests are diverse, including increased human population, expansion of the highway network, the use of modern hunting instruments, occupation history, the preference for game meat, availability of game substitutes, socioeconomic conditions, the degree of dependence on hunting as a food source, and cultural conditions (Bennet and Robinson 2000;Bodmer et al 1996;Constantino 2016;Constantino et al 2008;Jerozolimski and Peres 2003;Schenck et al 2006;Van Vliet and Mbazza 2011;Wright et al 2007). However, although hunting is responsible for supplementing the diet of various traditional populations (Fernandes-Ferreira and Alves 2014;Silva-Neto 2016), the demand for game meat in the tropical region by traditional, urban and rural populations has been putting pressure on wild species, making it difficult to recover their numbers (Alves et al 2009a;Alves et al 2016;Pérez 2009;Ramos et al 2016;Sousa and SrbekAraújo 2017) and provoked several local extinctions (Canale et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Wildlife Consumption Dynamics: Unveiling Conduru Park in Southern Bahia, Brazil

Vicente dos Santos Teixeira,

Duarte da Rocha,

Silveira Miranda

et al. 2024

Ethnobio Conserv

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The current investigation aimed to identify the wild animals utilized as a food source in five locations within the Serra do Conduru State Park region, Bahia, Brazil. The field survey was conducted from June 2016 to July 2017, involving semi-structured interviews and informal conversations with 45 hunters. The composition of species used for sustenance in the five locations was characterized through permutation multivariate analyses of variance. Generalized linear models were constructed to evaluate whether socio-demographic variables among hunters influenced the number of captured species. A total of 67 species (34 families and 22 orders) of hunting significance were documented in the region, with 41 being hunted for consumption. The most represented taxa were mammals (32), birds (21), reptiles (13), and amphibians (1). Dicotyles tajacu, Dasypus novemcinctus, and Cuniculus paca emerged as the most targeted species for food. Hunters who still reside within the conservation unit capture a greater number of wild animals. Older hunters and those with smaller family sizes hunt a broader range of species. The rifle and domestic dogs are the predominant techniques employed in the region. The findings underscore the persistence of illegal hunting practices in the Serra do Conduru State Park region. This emphasizes the necessity for measures directed at the conservation of hunted species, particularly those identified as being under some degree of threat in nature.

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Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: implications for the monitoring of illegal hunting and wildlife trade

Cited by 8 publications

References 15 publications

The phylogenetics of Leucocytozoon caulleryi infecting broiler chickens in endemic areas in Indonesia

The phylogenetics of Leucocytozoon caulleryi infecting broiler chickens in endemic areas in Indonesia

Optimization of polymerase chain reaction for the identification of Roe deer, Saiga, and Siberian stag living in Kazakhstan

Wildlife Consumption Dynamics: Unveiling Conduru Park in Southern Bahia, Brazil

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