Diagnostic d’infection à Helicobacter pylori sur biopsies gastriques : coloration standard, coloration spéciale ou immunohistochimie ?

Benoit, Anaïs; Hoyeau, Nadia; Fléjou, Jean‐François

doi:10.1016/j.annpat.2018.03.009

Cited by 18 publications

(12 citation statements)

References 16 publications

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“…Moreover, IHC is recommended when other routine methods fail to detect H. pylori in cases of chronic gastritis caused due to minimal infection or atypical distribution of bacteria in affected tissue. 27 The limitation of IHC staining for routine diagnostic application is high cost. Hence, IHC staining is recommended for cases with minimal H. pylori infection.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of immunohistochemical staining in detecting <em>Helicobacter pylori</em> in Saudi patients with minimal and atypical infection

et al. 2021

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Gastric Helicobacter pylori infection is diagnosed based on histopathological evaluation of gastric mucosal biopsies, urease test, urea breath test, H. pylori culturing, or direct detection using polymerase chain reaction (PCR). This study aimed to evaluate the efficacy of immunohistochemical (IHC) staining in detecting H. pylori in gastric biopsies from patients with chronic gastritis and minimal or atypical infection. Gastric biopsies from 50 patients with chronic gastritis were subjected to routine haematoxylin and eosin (H&E), modified Giemsa, and IHC staining. The results of staining were compared with those of quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis identified 32 (64%) H. pylori-positive cases, whereas IHC, H&E, and modified Giemsa staining identified 29 (58%), 27 (54%), and 21 (42%) positive cases. The sensitivity of IHC staining (87.50%) was higher than that of H&E (59.38%) and modified Giemsa (43.75%) staining. The specificity of H&E, modified Giemsa, and IHC staining was 55.56%, 61.11%, and 94.44%, respectively. IHC staining exhibited the highest diagnostic accuracy (90%), followed by H&E (58%) and modified Giemsa (50%) staining. Active gastritis, intestinal metaplasia, and lymphoid follicles were detected in 32 (64%), 4 (8%), and 22 (44%) cases, respectively, and all of these cases were H. pylori positive. In contrast to routine H&E and modified Giemsa staining, IHC allows for the accurate H. pylori detection in cases with minimal or atypical infection. Moreover, IHC can be an alternative diagnostic method to qRT-PCR for detection of H. pylori in such cases.

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Section: Discussionmentioning

confidence: 99%

Efficacy of immunohistochemical staining in detecting <em>Helicobacter pylori</em> in Saudi patients with minimal and atypical infection

et al. 2021

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Section: Histologymentioning

confidence: 99%

“…Additional staining in gastric biopsies was investigated, such as using cresyl violet or immunohistochemistry for H. pylori detection [19][20][21]. Benoit A et al [20] reported that it is not necessary to use this method to detect a H. pylori infection since conventional selective stains show good diagnostic accuracy. Immunohistochemistry can be used in cases of low bacterial density, atrophic gastritis with extensive intestinal metaplasia, and chronic active gastritis without H. pylori identification by standard staining.…”

Section: Histologymentioning

confidence: 99%

Current Helicobacter pylori Diagnostics

et al. 2021

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The high prevalence of Helicobacter pylori and the variety of gastroduodenal diseases caused by this pathogen necessitate the use of only accurate methods both for the primary diagnosis and for monitoring the eradication effectiveness. There is a broad spectrum of diagnostic methods available for detecting H. pylori. All methods can be classified as invasive or non-invasive. The need for upper endoscopy, different clinical circumstances, sensitivity and specificity, and accessibility defines the method chosen. This article reviews the advantages and disadvantages of the current options and novel developments in diagnostic tests for H. pylori detection. The progress in endoscopic modalities has made it possible not only to diagnose precancerous lesions and early gastric cancer but also to predict H. pylori infection in real time. The contribution of novel endoscopic evaluation technologies in the diagnosis of H. pylori such as visual endoscopy using blue laser imaging (BLI), linked color imaging (LCI), and magnifying endoscopy is discussed. Recent studies have demonstrated the capability of artificial intelligence to predict H. pylori status based on endoscopic images. Non-invasive diagnostic tests such as the urea breathing test and stool antigen test are recommended for primary diagnosis of H. pylori infection. Serology can be used for initial screening and epidemiological studies. The histology showed its value in detecting H. pylori and provided more information about the degree of gastric mucosa inflammation and precancerous lesions. Molecular methods are mainly used in detecting antibiotic resistance of H. pylori. Cultures from gastric biopsies are the gold standard and recommended for antibiotic susceptibility tests.

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“…As the widespread use of proton pump inhibitors (PPIs) may result in atypical presentation of gastritis, or in density variation of bacteria at different sites [ 37 ], the accuracy of histologic diagnosis of H. pylori infection can be improved by using special staining techniques, specific immune stain, or digital pathology [ 38 , 39 ]. However, a recent study reported a high percentage (94%) of H. pylori detection with the standard hematoxylin-eosin staining, compared with special staining [ 40 ].…”

Section: Invasive Testsmentioning

confidence: 99%

What Is New in Helicobacter pylori Diagnosis. An Overview

Dore

2021

JCM

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Helicobacter pylori infection remains one of the most prevalent infections worldwide, especially in low-resource countries, and the major risk factor for peptic ulcer and gastric cancer. The “test-and-treat” strategy is recommended by several guidelines and consensus. The choice of testing method is based on patient age, presence of alarm signs and/or symptoms, use of non-steroidal anti-inflammatory drugs, as well as local availability, test reliability, and cost. Culture is the gold standard to detect H. pylori and, possibly, to perform susceptibility testing, however, it requires upper endoscopy and dedicated labs. Recent advances in molecular biology have provided new strategies in detecting infection and antimicrobial resistance without invasive tests. In this review we attempt to offer a comprehensive panorama on the new diagnostic tools and their potential use in clinical settings, in order to accomplish specific recommendations.

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Diagnostic d’infection à Helicobacter pylori sur biopsies gastriques : coloration standard, coloration spéciale ou immunohistochimie ?

Cited by 18 publications

References 16 publications

Efficacy of immunohistochemical staining in detecting <em>Helicobacter pylori</em> in Saudi patients with minimal and atypical infection

Efficacy of immunohistochemical staining in detecting <em>Helicobacter pylori</em> in Saudi patients with minimal and atypical infection

Current Helicobacter pylori Diagnostics

What Is New in Helicobacter pylori Diagnosis. An Overview

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