Diagnostic Efficiency of Adsorbed Anthrax Vegetative Fluorescent Immunoglobulins Demonstrated in the Medical Trials

Sayapina, L. V.; Абдрашитова, А. С.; Lobach, R. N.; Komratov, A. V.; Malakhaeva, A. N.; Lyashova, O. Yu.; Valova, T. V.; Khramov, Mykyta; Gefan, N. G.

doi:10.21055/0370-1069-2012-4-92-96

Cited by 3 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Известные сибиреязвенные бактериофаги, выпускаемые в Российской Федерации, различаются по широте спектра лизируемых ими штаммов возбудителя сибирской язвы, а также по специфичности их действия [1,4]. Некоторую противоречивость отдельных авторов по этому вопросу можно объяснять использованием ими различных вариантов бактериофагов, отличающихся концентрацией фаговых частиц, недостаточной представительностью использованных выборок культур B. anthracis и близкородственных сапрофитов, а также различиями в оценке результатов теста на фагочувствительность.…”

unclassified

Development of Biotechnology for Anthrax Bacteriophages Reproduction, and Comparative Analysis of Their Properties Stability and Diagnostic Value

Головинская¹,

Tsygankova²,

Куличенко³

2014

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

Carried out has been complex comparative analysis of the production and diagnostic properties of such anthrax bacteriophages as Gamma A-26, Fah-RRIVV&M (Russian Research Institute of Veterinary Virology and Microbiology), R/D-Ph-6, BA-9, K RRIEV (Russian Research Institute of Experimental Veterinary), Saratov, and 186. It is revealed that all of the bacteriophages have the properties necessary for using them for preparation manufacturing purposes: they readily proliferate in liquid cultures of avirulent Bacillus anthracis strain-STI, and retain sufficient specific activity if stored at the optimum temperatures or, when exposed to short-term temperature excursions, within a period of two years. Most clearly expressed are the differences in diagnostic properties of the bacteriophages-specific activity and specificity. Put forward are the groups of bacteriophages that are the most relevant for identification or phage-typing of B. anthracis strains.

show abstract

unclassified

Development of Biotechnology for Anthrax Bacteriophages Reproduction, and Comparative Analysis of Their Properties Stability and Diagnostic Value

Головинская¹,

Tsygankova²,

Куличенко³

2014

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

show abstract

Development of luminescent serum for diagnostics of animal bacillus anthracix agent

et al. 2020

View full text Add to dashboard Cite

The purpose of the study was to obtain luminescent serum based on highly purified anthracoid globulins to diagnose anthrax agents in animals. To date, there are plenty diagnostic agents that allow rapid and accurate diagnostics of infectious diseases of animals. One of them is the luminescent microscopy of the fluorescent antibody method, which is used as an express method and provides for diagnostics within 3-5 hours. Hyperimmune serum globulins prepared on two types of antigens – protective from strain 55 (VNIIVViM) and capsular from Lange-2 strain at five-fold scheme of introduction of these antigens – were used to make luminescent anthracoid serum. The luminescent serum made on the basis of highly purified anthracoid globulins has a coloring titer (specific activity) of 1:16. When examining the specificity of the obtained luminescent serum in smears from anthrax agent strains, clear fluorescence was observed with more intense luminescence along the periphery of microbial cells.

show abstract

Comparative analysis of the results of live anthrax vaccine identification by immunofluorescence and immunochromatography

Alekseeva,

Kasina,

Nemirovskaya

2024

Biological Products. Prevention, Diagnosis, Treatment

View full text Add to dashboard Cite

INTRODUCTION. The quality evaluation of live anthrax vaccines will benefit from the implementation of alternative testing methods that are capable of specific identification of the Bacillus anthracis spore antigen using the appropriate diagnostic products that are authorised in the Russian Federation for the detection of B. anthracis spores.AIM. This study aimed to investigate the applicability of immunofluorescence analysis to the identification of live anthrax vaccines and compare this method with immunochromatography.MATERIALS AND METHODS. The study used commercial batches of a live anthrax vaccine and Russian diagnostic products, including diagnostic dry adsorbed fluorescent anti-anthrax spore immunoglobulins and an immunochromatographic assay (ICA) reagent kit for rapid detection and identification of B. anthracis spores (ICA system for B. anthracis). For smears and ICA system reactions, the authors prepared working solutions of bacterial suspensions at spore concentrations typical of the B. anthracis STI-1 vaccine strain. The spore concentrations were achieved using the pharmacopoeial reference standard (RS) for the opacity of bacterial suspensions of 10 international units (IU). Identification reactions involved the registration of immune complex formation.RESULTS. Immunofluorescence tests of the live anthrax vaccine demonstrated bright greenish-yellow envelope fluorescence with an intensity score of 3+ to 4+ for smears stained with diagnostic immunoglobulins at 1:32 and 1:64 dilutions. Immunochromatographic tests of the live anthrax vaccine detected the spore antigen at vaccine concentrations of 109 and 108 spores/mL, with the test strips showing two distinct dark-pink lines indicative of immunoprecipitation. According to the results obtained using the selected methods, the tested microbial culture was confirmed as B. anthracis.CONCLUSIONS. Immunochromatography and immunofluorescence tests with appropriate diagnostic preparations are convenient and reliable tools for the species-specific detection of B. anthracis STI-1 spores in the live anthrax vaccine. The results obtained in the anthrax vaccine identification tests provide a basis for recommending the above methods as supplementary alternatives to Ziehl–Neelsen bacteriological staining, which is currently prescribed by the State Pharmacopoeia of the Russian Federation.

show abstract

Diagnostic Efficiency of Adsorbed Anthrax Vegetative Fluorescent Immunoglobulins Demonstrated in the Medical Trials

Cited by 3 publications

References 1 publication

Development of Biotechnology for Anthrax Bacteriophages Reproduction, and Comparative Analysis of Their Properties Stability and Diagnostic Value

Development of Biotechnology for Anthrax Bacteriophages Reproduction, and Comparative Analysis of Their Properties Stability and Diagnostic Value

Development of luminescent serum for diagnostics of animal bacillus anthracix agent

Comparative analysis of the results of live anthrax vaccine identification by immunofluorescence and immunochromatography

Contact Info

Product

Resources

About