Diagnostic identification of Taenia saginata with the polymerase chain reaction

Gottstein, Bruno; Deplazes, Peter; Tanner, I.; Skaggs, Jennifer

doi:10.1016/0035-9203(91)90042-w

Cited by 35 publications

(13 citation statements)

References 5 publications

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“…Different improved postmortem diagnostic methods have been proposed, such as ELISA methods, detecting antigens in meat juice (Abuseir et al, 2007), immunohistochemical techniques, using the avidin-biotin complex immunohistochemical method (Ogunremi et al, 2004), and biomolecular assays (Gottstein et al, 1991;Abuseir et al, 2006;Geysen et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Development of a Biomolecular Assay for Postmortem Diagnosis ofTaenia saginataCysticercosis

Chiesa

Dalmasso

Bellio

et al. 2010

Foodborne Pathogens and Disease

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Bovine cysticercosis is caused by the larval stage of the human tapeworm Taenia saginata. According to European data on meat inspection, the prevalence ranges from 0.007% to 6.8%, but the real prevalence is considered to be at least 10 times higher. Laboratory confirmation of the etiological agent is based on gross, stereomicroscopic, and histological examination of submitted specimens. False identifications may occur, possibly because of death and degeneration of cysts, or because taeniid larvae and other tissue parasites, such as Sarcocystis spp., may cause similar macroscopic morphological lesions. Therefore, tests that can warrant sure identification of taeniid lesions and calcified cysts in the muscle are needed. The focus of our study was to develop a suitable postmortem test that could be applied on putative lesions by T. saginata cysticerci, as ambiguously diagnosed after routine meat inspection. In particular, we proposed a biomolecular assay targeting the mitochondrial cytochrome c oxidase subunit I gene (COI). For developing the polymerase chain reaction assay, viable cysts of Cysticercus bovis (n = 10) were used as positive reference samples, and those of Echinococcus granulosus (n = 3), Cysticercus tenuicollis (n = 3), and Sarcocystis spp. (n = 4) as reference negative controls. Further, to evaluate the applicability of the proposed assay, 171 samples of bovine muscular tissue, obtained from local slaughterhouses and containing lesions recognized as T. saginata cysticerci by macroscopic examination, were tested. The proposed test confirmed the diagnosis at postmortem inspection in 94.7% (162/171) of samples. In conclusion, the assay developed in this study, amplifying a short fragment from the mitochondrial gene COI, showed to be suitable for samples containing both viable and degenerating T. saginata cysticerci, yielding an unequivocal diagnosis.

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Section: Introductionmentioning

confidence: 99%

Development of a Biomolecular Assay for Postmortem Diagnosis ofTaenia saginataCysticercosis

Chiesa

Dalmasso

Bellio

et al. 2010

Foodborne Pathogens and Disease

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“…Ribosomal, mitochondrial, repetitive DNA sequences have been used in the development of different PCR (polymerase chain reaction) protocols (Harrison et al 1990;Gottstein et al 1991;Zarlenga et al 1991;Bowles and McManus 1994). Multiplex PCRs, PCR-RFLP (PCRrestriction fragment length polymorphism), and PCR sequencing have allowed the species-specific identification of T solium, T. saginata, and T. asiatica (Mayta et al 2000;González et al 2000González et al , 2010Jeon et al , 2011Jeon and Eom 2013).…”

Section: Molecular Diagnosismentioning

confidence: 99%

Taeniosis and Cysticercosis

Ferrer

Gárate

2014

Helminth Infections and Their Impact on Global Public Health

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Taeniosis and cysticercosis are zoonotic diseases produced by Taenia saginata and Taenia solium. The adult tapeworms are parasites of human intestine and show a wide geographical distribution. Taenia asiatica, another tapeworm species, was described in Southeast Asia. The larval stages of these cestodes (metacestodes or cysticerci) cause cysticercosis; T. saginata causes bovine cysticercosis, T. asiatica larvae develop in the pig viscera, and T. solium is able to produce cysticercosis in both pig and man. When the parasite larva invades, the central nervous system (CNS) can provoke neurocysticercosis (NCC), one of the most frequent parasitic infections of human CNS. These diseases continue to cause health problems and livestock industry losses in areas where the parasites are endemic and also in non-endemic regions as a consequence of travel and migrations. There are few symptoms associated with taeniosis; in contrast, NCC (pleomorphic pathology) could be a life-threatening disease, depending on the location, number, stage of cysticerci, and the host immune response. Diagnosis of taeniosis is generally achieved by stool microscopic examinations, and the detection of cysticercosis is generally performed by neuroimaging and immunoassays. Both conventional coprological techniques and immunological assays show limitations, and new diagnostic tools have been developed, more specific and sensitive, such as specific monoclonal antibodies, recombinant antigens, synthetic peptides, and PCR. Considering the clinical impact, veterinary problems, and economic losses derived from taeniosis/cysticercosis, control programs have been implemented. In addition, several vaccine candidates have been characterized that could complement the control measures.

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“…The utility of molecular markers derived from either mitochondrial or nuclear loci for the identification of human-Taenia has been amply demonstrated [29,30,[42][43][44][45]. These studies, however, have focused on a limited number of species in the genus, and a more synoptic comparative approach seems warranted in order to define patterns of genetic diversity and interspecific variation for Taenia spp.…”

Section: Diagnostics and Molecular Markersmentioning

confidence: 99%