Diagnostic laboratory immunology for talaromycosis (penicilliosis): review from the bench-top techniques to the point-of-care testing

Pruksaphon, Kritsada; Intaramat, Akarin; Ratanabanangkoon, Kavi; Nosanchuk, Joshua D.; Vanittanakom, Nongnuch; Youngchim, Sirida

doi:10.1016/j.diagmicrobio.2019.114959

Cited by 30 publications

(40 citation statements)

References 61 publications

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“…By immunoblot, the epitopes recognized by MAb 4D1 appeared to distribute among multiple undefined protein bands with broad high molecular mass, between 50 and 150 kDa ( Fig. 2b) similar to data previously described [7][8][9][10] . These results indicate that the target epitope of MAb 4D1 is shared by various forms of the glycoprotein according to previously described findings 12 .…”

Section: Immunoreactivity Patterns and Carbohydrate Components Of Thesupporting

confidence: 84%

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Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei

Pruksaphon

Ching

Nosanchuk

et al. 2020

Sci Rep

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Talaromyces marneffei is a dimorphic fungus that has emerged as an opportunistic pathogen particularly in individuals with HIV/AIDS. Since its dimorphism has been associated with its virulence, the transition from mold to yeast-like cells might be important for fungal pathogenesis, including its survival inside of phagocytic host cells. We investigated the expression of yeast antigen of T.marneffei using a yeast-specific monoclonal antibody (MAb) 4D1 during phase transition. We found that MAb 4D1 recognizes and binds to antigenic epitopes on the surface of yeast cells. Antibody to antigenic determinant binding was associated with time of exposure, mold to yeast conversion, and mammalian temperature. We also demonstrated that MAb 4D1 binds to and recognizes conidia to yeast cells’ transition inside of a human monocyte-like THP-1 cells line. Our studies are important because we demonstrated that MAb 4D1 can be used as a tool to study T.marneffei virulence, furthering the understanding of the therapeutic potential of passive immunity in this fungal pathogenesis.

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Section: Immunoreactivity Patterns and Carbohydrate Components Of Thesupporting

confidence: 84%

“…MAb 4D1 was generated in the pre-proteomes and recombinant protein technology era 7,10 and the antigenic target recognized by this clone is unknown. Purification of MAb 4D1 target proteins from numerous contaminating proteins in TM CYA have been attempted by liquid isoelectric focusing (IEF).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei

Pruksaphon

Ching

Nosanchuk

et al. 2020

Sci Rep

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“…When the level of IgM reaches the detection limit of 62 the assay kit, the detection of IgM can avoid false-negative results owing to sampling. At present, the main 63 methods for the detection of specific antibodies in clinical laboratories are gold immunochromatography 64 assay (GICA) and enzyme-linked immunosorbent assay (ELISA) [7][8][9][10][11], both of which have the advantages 65 of mature methodology, high flux detection, simple operation, rapid detection, no special equipment, and 66 they are low cost. Using these two methods to detect SARS-CoV-2 IgM can identify or screen 67 SARS-CoV-2 infection in suspicious and close contact populations earlier, more quickly and effectively, 68 and improve the accuracy of epidemiological monitoring, which is very important for patient management 69 and epidemic prevention and control.…”

Section: Introduction 46mentioning

confidence: 99%

A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays

et al. 2020

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We set out to investigate the interference factors that led to false-positive novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM detection results using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions. GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (Flu B) IgM-positive sera, 5 Mycoplasma pneumoniae IgM-positive sera, 5 Legionella pneumophila IgM-positive sera, 6 sera of HIV infection patients, 36 rheumatoid factor IgM (RF-IgM)-positive sera, 5 sera from hypertensive patients, 5 sera from diabetes mellitus patients, and 14 sera from novel coronavirus infection disease 19 (COVID-19) patients. The interference factors causing false-positive reactivity with the two methods were analyzed, and the urea dissociation test was employed to dissociate the SARS-CoV-2 IgM-positive serum using the best dissociation concentration. The two methods detected positive SARS-CoV-2 IgM in 22 mid-to-high-level-RF-IgM-positive sera and 14 sera from COVID-19 patients; the other 50 sera were negative. At a urea dissociation concentration of 6 mol/liter, SARS-CoV-2 IgM results were positive in 1 mid-to-high-level-RF-IgM-positive serum and in 14 COVID-19 patient sera detected using GICA. At a urea dissociation concentration of 4 mol/liter and with affinity index (AI) levels lower than 0.371 set to negative, SARS-CoV-2 IgM results were positive in 3 mid-to-high-level-RF-IgM-positive sera and in 14 COVID-19 patient sera detected using ELISA. The presence of RF-IgM at mid-to-high levels could lead to false-positive reactivity of SARS-CoV-2 IgM detected using GICA and ELISA, and urea dissociation tests would be helpful in reducing SARS-CoV-2 IgM false-positive results.

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“…Antigen detection is a promising field to aid in earlier diagnosis of Talaromyces with impressive progress in recent years [65,116,[119][120][121]. A lateral flow immunochromatographic assay utilizing a monoclonal antibody, 4D1, conjugated with gold colloid as a signal generator and lined with T. marneffei cytoplasmic yeast antigen detected antigen in urine with a sensitivity of 87.9% and specificity of 100% in persons with confirmed T. marneffei infection by blood culture [64].…”

Section: Talaromycosismentioning

confidence: 99%

Diagnosis of Pulmonary Infections Due to Endemic Fungi

et al. 2021

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Endemic mycoses including Histoplasma, Blastomyces, Coccidioides, Paracoccidioides, and Talaromyces are dimorphic fungi that can cause a variety of clinical manifestations, including respiratory infections. Their pulmonary presentations are variable, and diagnosis is often delayed as they can mimic other infectious and non-infectious causes of pulmonary disease. Delay in diagnosis can lead to unnecessary antibiotic use, repeat hospitalizations, and increased morbidity and mortality. The diagnosis of endemic fungal pulmonary infections often relies on multiple diagnostic tests including culture, tissue histopathology, antigen assays, and antibody assays. Due to the increased use of immunosuppressive agents and the widening geographic ranges where these infections are being found, the prevalence of endemic fungal infections is increasing. Physicians need to be aware of the clinical manifestations of pulmonary infections due to endemic fungal in order to ensure that the proper diagnostic work up is obtained promptly. A high index of suspicion is particularly important in patients with suspected pulmonary infections who have failed to improve despite antibiotics in the appropriate setting. We present a review diagnostic testing for pulmonary infections due to endemic mycoses.

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Diagnostic laboratory immunology for talaromycosis (penicilliosis): review from the bench-top techniques to the point-of-care testing

Cited by 30 publications

References 61 publications

Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei

Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei

A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays

Diagnosis of Pulmonary Infections Due to Endemic Fungi

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