Diagnostic performance of HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR assays on formalin-fixed paraffin-embedded cervical tissue specimens from women with cervical cancer

Wang, Hye young; Kim, Geehyuk; Cho, Hyemi; Kim, Sung-Hyun; Lee, Dongsup; Park, Sunyoung; Park, Kwang Hwa; Lee, Hyeyoung

doi:10.1016/j.yexmp.2015.03.036

Cited by 15 publications

(21 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, our study results are not only in accordance with those of several other groups who have identified telomerase activity in the majority of malignant tumors, but also in line with those who have identified telomerase activity in proliferative diseases, predisposing the development of malignant tumors [28][29][30][31][32] .…”

Section: Discussionsupporting

confidence: 92%

“…However, when quantifying telomerase activity, it became significantly higher according to cervical lesion reported. These results support previous reports, in which telomerase activity levels seem to be related to the pathologic stages of different types of cancers including hepatocellular carcinoma (HCC) 28 , where human telomerase reverse transcriptase (hTER) RNA expression showed a statistically significant relationship with tumor size in HCC patients regarding a cervical lesion 24,29 . A correlation between telomerase activity or hTER expression and the varying degrees of cervical lesions was observed regarding potentially malignant oral lesions.…”

Section: Discussionsupporting

confidence: 91%

“…Studies reporting a positive correlation between telomerase activity and pathologic degree of tumor [29][30] suggest that the cells with higher telomerase activity…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Castro-Duque¹,

Loango-Chamorro²,

Ruiz-Hoyos³

et al. 2015

Rev. Bras. Ginecol. Obstet.

View full text Add to dashboard Cite

PURPOSE To analyze the relation between the cytological findings and telomerase activity (TA). METHODS Cervical samples were evaluated and classified according to the Bethesda System. Telomerase activity was measured total product generated values (TPG) using the TRAP assay (telomeric repeat amplification protocol); data were analyzed statistically using the χ2 test, with the level of significance set at p<0.05. RESULTS The study was conducted on 102 patients. Of these, 3.9% showed normal cytological findings, 8.8% showed cervicitis; 2% showed Atypical Squamous Cells of Undetermined Significance (ASCUS); 67.6% showed Low Grade Squamous Intraepithelial Lesion (LSIL); 11.8% showed High Grade Squamous Intraepithelial Lesion (H-SIL) and 5.9% showed Squamous Carcinoma. Among telomerase-positive samples, the TPG values were cervicitis

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Castro-Duque¹,

Loango-Chamorro²,

Ruiz-Hoyos³

et al. 2015

Rev. Bras. Ginecol. Obstet.

View full text Add to dashboard Cite

show abstract

“…A World Health Organization report revealed that >270,000 women succumbed to CC in 2014 and >85% of cases occur in less developed regions (28). The morbidity and mortality of CC are high (29) and the age of onset is a decreasing (30,31). In the past few decades, great progress has been made researching treatments for CC (32,33).…”

Section: Discussionmentioning

confidence: 99%

miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3

Zhang

et al. 2018

Exp Ther Med

View full text Add to dashboard Cite

Cervical cancer (CC) is one of the most malignant tumors that affect women. Recent studies have reported that microRNAs (miRs) serve important roles in CC. The aim of the present study was to investigate the role of miR-218 in CC and to verify its underlying mechanism. The results of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) revealed that miR-218 was dramatically downregulated in CC tissues and cell lines. Furthermore, the expression of Gli3 and Ki67 was measured using RT-qPCR and the results revealed that levels of these proteins were negatively correlated with miR-218 in CC tissues. The protein expression levels were determined by western blotting. Then SiHa cell line was used to investigate the mechanism of CC. Following cell transfection, cell apoptosis and cycle analyses were performed using the flow cytometry. The results revealed that miR-218 overexpression significantly inhibited cell proliferation, apoptosis and cell cycle. Additionally, luciferase reporter assay revealed that Gli3 may be a novel and direct target of miR-218 in CC. Taken together, the results of the present study suggest that miR-218 overexpression or Gli3 knockdown may have potential as therapeutic strategies for the treatment of CC.

show abstract

“…The biomarkers p16, Ki-67, human telomerase reverse transcriptase (hTERT), and L1 capsid protein hold clinical promise for more accurate diagnostic workup of patients with positive HPV testing. While p16 is overexpressed in cervical dysplasia and associated with high-risk HPV oncogenic transformation, Ki-67 and hTERT are markers of uncontrolled cell proliferation [13,39]. Meanwhile, the presence of HPV L1 capsid protein within dysplastic cells demonstrates a completed HPV life cycle, and HPV L1 capsid protein-negative cases have lost the capacity to synthesize virions [40].…”

Section: Future Screening Strategiesmentioning

confidence: 99%

Current Cervical Carcinoma Screening Guidelines

Schlichte

Guidry

2015

JCM

View full text Add to dashboard Cite

A formidable threat to the health of women, cervical carcinoma can be prevented in many cases with adequate screening. The current guidelines for cervical carcinoma screening were created as joint recommendations of the American Cancer Society (ACS), the American Society for Colposcopy and Cervical Pathology (ASCCP) and the American Society for Clinical Pathology (ASCP) in 2012, and later accepted and promoted by the American Congress of Obstetricians and Gynecologists (ACOG). The 2012 recommendations underscore the utility of molecular testing as an adjunct to cytology screening for certain women and provide guidance to clinicians based on different risk-benefit considerations for different ages. This manuscript will review screening techniques and current recommendations for cervical cancer screening and human papilloma virus (HPV) testing, as well as possible future screening strategies.

show abstract

Diagnostic performance of HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR assays on formalin-fixed paraffin-embedded cervical tissue specimens from women with cervical cancer

Cited by 15 publications

References 19 publications

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3

Current Cervical Carcinoma Screening Guidelines

Contact Info

Product

Resources

About