Diagnostic relevance of cell block procedure in secondary tumors of the pancreas

Aquaporin‐1 expression in fluoro‐edenite‐induced mesothelioma effusions: An approach by cell‐block procedure

“…Cytological evaluation of PE is employed to achieve a definite diagnosis of MM. For this purpose, the CB procedure is an excellent technical support since it allows cell architecture preservation, routine stains as well as IHC or molecular analyses . Cytological and IHC assessments of MM through specific panels of antibodies are available, but prognostic markers are less numerous and not routinely used in MM effusions.…”

“…For CB preparation, after rapid on‐site evaluation (ROSE) of specimen adequacy and preliminary cytological diagnosis by Diff‐Quik® stain, the 22‐gauge needles and syringes used and the residual material were rinsed with 10 mL of 50% ethanol into a special container. The sample was centrifuged to create pellets (in a 10 mL disposable centrifuge tube at 2147 g for 6 minutes), which were immediately fixed in 4% neutral buffered formalin for 45 minutes at room temperature, as reported elsewhere . Pellets were then placed in a cassette and stored at 80% ethanol until ready for processing in an automatic tissue processor (Leica TP 1020 Leica Microsystems, Wetzlar, Germany) .…”

“…The sample was centrifuged to create pellets (in a 10 mL disposable centrifuge tube at 2147 g for 6 minutes), which were immediately fixed in 4% neutral buffered formalin for 45 minutes at room temperature, as reported elsewhere . Pellets were then placed in a cassette and stored at 80% ethanol until ready for processing in an automatic tissue processor (Leica TP 1020 Leica Microsystems, Wetzlar, Germany) . The obtained CBs were embedded in paraffin at 56°C, cut into 3‐μm thick sections and routinely stained with haematoxylin‐eosin; parallel serial sections of the same thickness were mounted on poly‐lysine‐coated glasses and used for IHC together with slides routinely obtained from the corresponding thoracoscopic biopsy samples.…”

“…The cytological MM diagnosis on MPE can be performed on direct fixed smears, liquid‐based cytology or cell‐block (CB), the latter is the most effective method . CB better preserves cellular architecture, and corresponding IHC is highly effective and accurate, reproducing the technical conditions of histological samples . Many diagnostic biomarkers are used for MM diagnosis, such as calretinin, CK5, podoplanin, mesothelin, osteopontin and others, but prognostic predictors are not routinely applied .…”

Comparison of Diagnostic Yield of Endoscopic Ultrasound-guided Fine-needle Aspiration Cytology and Cell Block in Solid Lesions

“…The sections obtained from CB can be stained with hematoxylin and eosin (H and E) and subjected to immunohistochemistry (IHC) or molecular analyses. [10,11] Due to limited availability of on-site pathologist, multiple passes are made to increase the chance of collecting adequate sample. Making a CB from the material obtained may improve the yield, and in this study, we aim to compare individual and combined diagnostic yields of CB and cytology in solid lesions in the absence of on-site pathologist while following a protocol-based approach.…”