Diagnostic significance of immunological markers with various forms of ulcerative colitis

Ткачев, А В; Mkrtchyan, Lilit; Беловолова, Р. А.; Devlikamova, Tatiana

doi:10.14300/mnnc.2019.14081

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…The studies addressing the subpopulation composition of circulating leukocytes in patients with IBD compared to healthy volunteers are also limited. Thus, for example, A. Tkachev et al demonstrated an increase in the total number of T-lymphocytes and B-lymphocytes in 44 patients with UC compared with 20 healthy volunteers [20]. In a study by Y.…”

Section: Discussionmentioning

confidence: 98%

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Khusainova,

Genkel,

Kuznetsova

et al. 2024

Gastroenterology Insights

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The aim of the study was to investigate the relationship between the serum zonulin and the cellular immunity in patients with ulcerative colitis (UC) and Crohn’s disease (CD). The study included 97 patients, 13 (13.4%) patients with CD and 84 (86.6%) patients with UC. The concentration of zonulin in the serum was studied using the ELISA kits. The investigation of the circulated leukocyte subpopulation was carried out through flow cytometry. The functional activity of the circulating neutrophils was studied using the absorption capacity of the monodisperse polystyrene latex particles and indicators of oxygen-dependent metabolism in the nitroblue tetrazolium test. The serum zonulin concentration in CD patients was significantly higher compared with UC patients (p = 0.003). The zonulin concentration directly correlated with the functional activity of the circulating neutrophils. Patients with a zonulin concentration > 472.4 pg/mL had a significantly higher number of band neutrophils (p = 0.0104), CD3+CD8+ cells (p = 0.0212), NK cells (p = 0.0161), and lower–CD19+ cells (p = 0.0034). Among the IBD patients, zonulin was associated with IBD severity. An increase in the serum concentration of zonulin was associated with an increase in the functional activity of circulating neutrophils and an increase in the number of CD3+CD8+ cells, NK cells, and a decrease in the number of CD19+ cells.

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Section: Discussionmentioning

confidence: 98%

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Khusainova,

Genkel,

Kuznetsova

et al. 2024

Gastroenterology Insights

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Immune changes at the injured sites in oxazolone-induced ulcerous colitis: Influence of ozone therapy

2022

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Impaired immunoregulation and development of autoimmune response to antigens of own intestinal microbiota and inflammation-altered antigens of colonic cells represent the key links in pathogenesis of inflammatory bowel diseases. Multimodal biological effects of ozone presunme the usage of local and systemic ozone therapy in complex treatment of many inflammatory diseases of the gastrointestinal tract. The aim of our work was to study effects of intraperitoneal and rectal ozone therapy upon immune parameters of the lesion focus in oxazolone-induced ulcerative colitis in the course of time. The study was carried out on 64 adult male inbred Wistar rats weighing 240±20 g. Experimental ulcerative colitis was produced by oxazolone treatment (4-ethoxymethylene-2-phenyl-2-oxazolin-5-one) (SigmaAldrich, USA). The ozone-oxygen mixture was injected intraperitoneally or rectally at a concentration of 1.0-1.2 mg/l, once a day, in a volume of 10 ml, at the 6-day course. The results of experiments were recorded on the days +2, +4 and +6. The concentrations of IL-17 and IL-23 was determined in a homogenate of intestinal tissues (Bender Medsystems, Austria) using a Personal LAB analyzer; expression of CD4 and FoxP3 on intestinal lymphocytes was determined by immunohistochemistry technique (ElisaKit, China). The observed tissue damage of large intestine showed an increase from day 2 to day 6 of oxazolone-induced ulcerative colitis. The total number of lymphocytes significantly increased upon development of experimental colitis, with parallel decrease in the number of CD4+ lymphocytes and FoxP3-positive T lymphocytes. IL-17 and IL-23 concentrations in the tissues increased with the severity of inflammatory changes in the lesion focus. Intraperitoneal ozone administration was associated with significant reduction of lymphocyte contents in the damaged tissues on the 6th day, whereas the numbers of CD4+ and FoxP3 positive T lymphocytes normalized by the 6th day. The levels of IL-17 and IL-23 increased from day 2 to day 6, with a lower IL-23 values on day 6 as compared with non-treated animals. Rectal administration of ACS led to the normalization of FoxP3 cells on the 6th day to the values of intact animals. The levels of proinflammatory cytokines (IL-17 and IL-23) significantly decreased on the 6th day as compared to the group of animals without treatment, which could be due to anti-inflammatory properties of ozone.

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Diagnostic significance of immunological markers with various forms of ulcerative colitis

Cited by 2 publications

References 8 publications

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Immune changes at the injured sites in oxazolone-induced ulcerous colitis: Influence of ozone therapy

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