Diagnostic significance of influenza subtype-specific IgG, IgA, and IgM antibodies

Döller, G; Döller, P. C.; Gerth, H. J.

doi:10.1016/0092-1157(86)90001-6

Cited by 7 publications

(8 citation statements)

References 20 publications

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“…These criteria were indirectly shown to be suitable, since 94% of the patients with influenza A antigen detected in NPS fulfilled the criteria and since among 11 cases with CF titers of .80 but without a fourfold titer rise 10 had high IgM HIT titers. According to our experience, and as reported by others, long persistence of CF antibodies to influenza A virus is uncommon (6).…”

Section: Discussionsupporting

confidence: 80%

“…The serum IgA antibody response to influenza A has previously been studied by others, with some diverging results (1,5,6,11,17). Burlington and co-workers (5) detected a significant IgA titer rise by indirect ELISA in 96% of patients with an infection with influenza A HlNi virus and in 74% in patients with an experimental influenza A H3N2 virus infection.…”

Section: Discussionmentioning

confidence: 99%

“…influenza A. The IgM capture technique has proven useful for detection of IgM in several different virus infections (6,8,18,23,29). One advantage of the method is the diminished risk of interference from rheumatoid factor, which is of major concern in the indirect ELISA (26).…”

Section: Discussionmentioning

confidence: 99%

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Diagnosis of influenza A virus infections by detection of specific immunoglobulins M, A, and G in serum

et al. 1989

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The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a fourfold rise in titer or high titers (.80) of influenza A virus antibodies by the complement fixation test. The IgM response, analyzed by the hemadsorption immunosorbent technique, was compared with the IgG and IgA responses analyzed by an indirect enzyme-linked immunosorbent assay and the hemadsorption immunosorbent technique, respectively. Antigen detection in nasopharyngeal secretions by immunofluorescence was performed for all patients on admission to hospital. Specific IgM was detected in 86% (55 of 64) of the patients with influenza A. In sera from 36% (18 of 64) of the patients it was detected already on admission. Influenza A virus antigen was detected in nasopharyngeal cells by immunofluorescence on admission in 53% (34 of 64) of the patients. A combination of immunofluorescence and IgM results gave a significantly higher diagnostic rate, 69% (P < 0.01), on admission than did each of the two tests separately. An IgA serum antibody response was seen in 76% (48 of 64) of the cases but did not contribute to any increase in the diagnostic rate. IgM detection by the hemadsorption immunosorbent technique was found to be a valuable supplement for the diagnosis of influenza A in an early phase of the disease.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Diagnosis of influenza A virus infections by detection of specific immunoglobulins M, A, and G in serum

et al. 1989

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“…In contrast to antibodies of the IgA and IgG isotypes, the diagnostic value of IgM antibodies in influenza virus infection seems to be limited. Although the measurement of IgM responses has been shown to be of diagnostic value in primary influenza virus infection (3,18), IgA and IgG antibody responses predominate in influenza virus-infected patients (5,7,11). Therefore, the measurement of IgG and IgA antibody responses is preferred for the serologic confirmation of influenza virus infections.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, ELISAs enable the detection of antibodies of different isotypes (3,6,18,19). For example, the demonstration of virus-specific IgA antibodies after influenza virus infections has been shown to be of diagnostic value (5,6,19). The preparation of viral antigens to be used in these ELISAs usually requires the concentration and purification of virus conventionally propagated in embryonated chicken eggs or cell culture.…”

mentioning

confidence: 99%

Use of Recombinant Nucleoproteins in Enzyme-Linked Immunosorbent Assays for Detection of Virus-Specific Immunoglobulin A (IgA) and IgG Antibodies in Influenza Virus A- or B-Infected Patients

Voeten

Groen²,

Alphen

et al. 1998

J Clin Microbiol

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The nucleoprotein genes of influenza virus A/Netherlands/018/94 (H3N2) and influenza virus B/Harbin/7/94 were cloned into the bacterial expression vector pMalC to yield highly purified recombinant influenza virus A and B nucleoproteins. With these recombinant influenza nucleoproteins, enzyme-linked immunosorbent assays (ELISAs) were developed for the detection of influenza virus A- and B-specific immunoglobulin A (IgA) and IgG serum antibodies. Serum samples were collected at consecutive time points after the onset of clinical symptoms from patients with confirmed influenza virus A or B infections. Nucleoprotein-specific IgA antibodies were detected in 41.2% of influenza virus A-infected patients and in 66.7% of influenza virus B-infected patients on day 6 after the onset of clinical symptoms. In serum samples taken on day 21 (influenza virus A-infected patients) or day 28 (influenza virus B-infected patients), nucleoprotein-specific IgA antibodies could be detected in 58.8 and 58.3% of influenza virus A- and B-infected patients, respectively. At the same time, IgG antibody rises were detected in 88.2% of influenza virus A-infected patients and in 95.8% of influenza virus B-infected patients. On comparison, hemagglutination inhibition assays detected antibody titer rises in 81.3 and 72.7% of patients infected with influenza viruses A and B, respectively. In contrast to the detection of nucleoprotein-specific IgG antibodies or hemagglutination-inhibiting antibodies, the detection of nucleoprotein-specific IgA antibodies does not require paired serum samples and therefore can be considered an attractive alternative for the rapid serological diagnosis of influenza.

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Determination of Antibody to Respiratory Pathogens

Schwartzman¹,

Kamforin²

1989

Reviews on Immunoassay Technology

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Qualitative evaluation and quantitative determination of antibody to respiratory pathogens represents a substantial part of research and applied virology. These investigations can serve quite different purposes. Epidemiologists need the serological data necessary for the control of influenza and other respiratory infections and for elucidating aetiological peculiarities of past infections. A system of constant surveillance of influenza morbidity and seroepidemiological status of the population has been organized in the USSR. The herd immunity is assessed as to circulating influenza viruses and some older strains which have gone out of circulation. The results obtained are important not only for current epidemiological analysis, but also because they enable the prognosis, to some extent, of the epidemic process, the evaluation of danger in shift of influenza viruses when their reappearance in the circulation is anticipated, as was the case in 1976 in the USA and in 1977 in China and in the USSR.Specialists who are developing new vaccines or improving existing ones are interested in determining the immunological status of those vaccinated. These results are essential for immunologists in order to elucidate mechanisms of humoral immunity. Based on serological information, the impact of chemoprophylactic and chemotherapeutic drugs on the immunological system can be evaluated. Determination of immune response to an infectious agent contributes essentially to a reliable diagnosis; as a result, the improvement of serological techniques is perpetuated in further investigations. It goes without saying that neither a single technique nor even a limited number of them can provide a solution to all the problems mentioned above.The purpose of this review is consideration of the main serological techniques available and their application in serological investigations of acute respiratory infections.

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Diagnostic significance of influenza subtype-specific IgG, IgA, and IgM antibodies

Cited by 7 publications

References 20 publications

Diagnosis of influenza A virus infections by detection of specific immunoglobulins M, A, and G in serum

Diagnosis of influenza A virus infections by detection of specific immunoglobulins M, A, and G in serum

Use of Recombinant Nucleoproteins in Enzyme-Linked Immunosorbent Assays for Detection of Virus-Specific Immunoglobulin A (IgA) and IgG Antibodies in Influenza Virus A- or B-Infected Patients

Determination of Antibody to Respiratory Pathogens

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