Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection

Fosgate, Geoffrey T.; Tavornpanich, Saraya; Hunter, D.; Pugh, Roberta; Sterle, J.A.; Schumann, Kate R.; Eberling, August J.; Beckham, Tammy R.; Martin, Bruce L.; Clarke, Neville P.; Adams, L. Garry

doi:10.1016/j.vetmic.2008.04.016

Cited by 7 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A comprehensive recent study of specificity of this method confirmed a very high specificity, but even with the inconclusive reactions considered false-positives, the specificity was 99.9%, which may provide enough accuracy for a diagnosis during an outbreak, but may not be specific enough for surveillance in FMDfree areas (23). The finding that 4 samples failed to yield a C T value throws doubt on the provenance of the tested material, because stored cell culturegrown antigens to four of the isolates readily reacted in the rRT-PCR which had a nucleotide substitution in the probe site, enabling it to escape recognition by the routine diagnostic probe.…”

Section: Discussionmentioning

confidence: 98%

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran

et al. 2013

View full text Add to dashboard Cite

Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological samples at the reference laboratory for FMD, Iran. Materials and Methods: Two different RT-PCR assays targeting two different regions 5´ untranslated region (5´-UTR) and RNA polymerase (3D) of the FMDV genome were used to confirm the presence of FMDV in epithelial suspensions. Results: In the two methods the viral RNA in all tested archival serotypes of FMDV were detected. Specificity of this reaction was confirmed by the use of swine vesicular disease virus and blue-tongue. The amount of cycle threshold (C T) value of all seven serotypes was different and the lowest and highest of C T value achieved for SAT3, A, O types and SAT2, C types, respectively. Conclusion: The results showed that RT-PCR was more sensitive and effective than routine diagnostic methods. Furthermore, RT-PCR as a strong and valuable tool concomitant with diagnostic routine methods facilitate monitoring the fields FMDV strains and suggested that the use of the multiple diagnostic targets could enhance the sensitivity of the molecular methods for the detection of FMDV.

show abstract

Section: Discussionmentioning

confidence: 98%

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran

et al. 2013

View full text Add to dashboard Cite

show abstract

“…However, samples from the area free of FMD, which were properly stored, were all negative by real-time RT-PCR as well as by virus isolation and ELISA, indicating a high specificity of this test as previously demonstrated [ 12 ]. A comprehensive recent study of specificity of this method confirmed a very high specificity, but even with the inconclusive reactions considered false-positives, the specificity was 99.9%, which may provide enough accuracy for a diagnosis during an outbreak, but may not be specific enough for surveillance in FMD-free areas [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil

Paixão

Neta

Paiva³

et al. 2008

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…The conventional RT-PCR and real-time RT-PCR have been widely used to specifically detect FMDV [ 19 , 20 ]. The purpose of this assay was to estimate the binding target virus efficiency of MNBs by real-time RT-PCR and conventional RT-PCR.…”

Section: Resultsmentioning

confidence: 99%

Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody

et al. 2015

View full text Add to dashboard Cite

BackgroundImmunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.ResultsIn this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples.ConclusionUltimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-015-0201-5) contains supplementary material, which is available to authorized users.

show abstract

Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection

Cited by 7 publications

References 19 publications

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran

Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil

Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody

Contact Info

Product

Resources

About