Diagnostic Value of Salivary miRNA in Head and Neck Squamous Cell Cancer: Systematic Review and Meta-Analysis

Kang, Jeong Wook; Eun, Young Gyu; Lee, Young Chan

doi:10.3390/ijms22137026

Cited by 35 publications

(26 citation statements)

References 38 publications

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Section: Discussionmentioning

confidence: 99%

“…Saliva is an increasingly attractive body fluid in the search for disease biomarkers [29][30][31][32]45,61]. miRNAs exhibit remarkable stability in severe conditions, such as extended storage [24,29,32,62]. Zheng et al demonstrated that saliva is not affected by coagulation that could induce a release of miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…The RNA was isolated from each saliva sample using the miRNeasy Kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer's instructions [29,31,44,45]. RNA quality was assessed using the Agilent Technologies Tapestation 2200.…”

Section: Rna Sample Extraction Preparation and Quality Controlmentioning

confidence: 99%

“…Similarly, saliva miRNAome analysis has been investigated by several teams exploring biomarkers for numerous benign and malignant disorders but never in the context of endometriosis [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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Salivary MicroRNA Signature for Diagnosis of Endometriosis

Bendifallah

Suisse²,

Puchar

et al. 2022

JCM

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Background: Endometriosis diagnosis constitutes a considerable economic burden for the healthcare system with diagnostic tools often inconclusive with insufficient accuracy. We sought to analyze the human miRNAome to define a saliva-based diagnostic miRNA signature for endometriosis. Methods: We performed a prospective ENDO-miRNA study involving 200 saliva samples obtained from 200 women with chronic pelvic pain suggestive of endometriosis collected between January and June 2021. The study consisted of two parts: (i) identification of a biomarker based on genome-wide miRNA expression profiling by small RNA sequencing using next-generation sequencing (NGS) and (ii) development of a saliva-based miRNA diagnostic signature according to expression and accuracy profiling using a Random Forest algorithm. Results: Among the 200 patients, 76.5% (n = 153) were diagnosed with endometriosis and 23.5% (n = 47) without (controls). Small RNA-seq of 200 saliva samples yielded ~4642 M raw sequencing reads (from ~13.7 M to ~39.3 M reads/sample). Quantification of the filtered reads and identification of known miRNAs yielded ~190 M sequences that were mapped to 2561 known miRNAs. Of the 2561 known miRNAs, the feature selection with Random Forest algorithm generated after internally cross validation a saliva signature of endometriosis composed of 109 miRNAs. The respective sensitivity, specificity, and AUC for the diagnostic miRNA signature were 96.7%, 100%, and 98.3%. Conclusions: The ENDO-miRNA study is the first prospective study to report a saliva-based diagnostic miRNA signature for endometriosis. This could contribute to improving early diagnosis by means of a non-invasive tool easily available in any healthcare system.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Rna Sample Extraction Preparation and Quality Controlmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Salivary MicroRNA Signature for Diagnosis of Endometriosis

Bendifallah

Suisse²,

Puchar

et al. 2022

JCM

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“…Despite the vast progress in the field of miRNAs as cancer biomarkers, the translation of results to a clinical setting is still challenging and usually lacks reproducibility [202]. However, research is still ongoing, and numerous miRNA molecules are investigated for their biomarker potential in many cancer types, including common and rare gynaecological [202,203], head and neck [204], gastrointestinal [205,206], and lung cancer [207]. Herein, the current knowledge regarding miRNAs as biomarkers for CC, which is the most associated with HPV, is summarized, and additional information for HNCs are provided.…”

Section: Mirna In Hpv-associated Cancermentioning

confidence: 99%

Human Papillomaviruses-Associated Cancers: An Update of Current Knowledge

Pešut

Đukić

Lulić

et al. 2021

Viruses

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Human papillomaviruses (HPVs), which are small, double-stranded, circular DNA viruses infecting human epithelial cells, are associated with various benign and malignant lesions of mucosa and skin. Intensive research on the oncogenic potential of HPVs started in the 1970s and spread across Europe, including Croatia, and worldwide. Nowadays, the causative role of a subset of oncogenic or high-risk (HR) HPV types, led by HPV-16 and HPV-18, of different anogenital and head and neck cancers is well accepted. Two major viral oncoproteins, E6 and E7, are directly involved in the development of HPV-related malignancies by targeting synergistically various cellular pathways involved in the regulation of cell cycle control, apoptosis, and cell polarity control networks as well as host immune response. This review is aimed at describing the key elements in HPV-related carcinogenesis and the advances in cancer prevention with reference to past and on-going research in Croatia.

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Salivary biomarkers in breast cancer diagnosis: A systematic review and diagnostic meta‐analysis

et al. 2022

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Background Salivary diagnostics and their utility as a nonaggressive approach for breast cancer diagnosis have been extensively studied in recent years. This meta‐analysis assesses the diagnostic value of salivary biomarkers in differentiating between patients with breast cancer and controls. Methods We conducted a meta‐analysis and systematic review of studies related to salivary diagnostics published in PubMed, EMBASE, Scopus, Ovid, Science Direct, Web of Science (WOS), and Google Scholar. The articles were chosen utilizing inclusion and exclusion criteria, as well as assessing their quality. Specificity and sensitivity, along with negative and positive likelihood ratios (NLR and PLR) and diagnostic odds ratio (DOR), were calculated based on random‐ or fixed‐effects model. Area under the curve (AUC) and summary receiver‐operating characteristic (SROC) were plotted and evaluated, and Fagan's Nomogram was evaluated for clinical utility. Results Our systematic review and meta‐analysis included 14 papers containing 121 study units with 8639 adult subjects (4149 breast cancer patients and 4490 controls without cancer). The pooled specificity and sensitivity were 0.727 (95% CI: 0.713–0.740) and 0.717 (95% CI: 0.703–0.730), respectively. The pooled NLR and PLR were 0.396 (95% CI: 0.364–0.432) and 2.597 (95% CI: 2.389–2.824), respectively. The pooled DOR was 7.837 (95% CI: 6.624–9.277), with the AUC equal to 0.801. The Fagan's nomogram showed post‐test probabilities of 28% and 72% for negative and positive outcomes, respectively. We also conducted subgroup analyses to determine specificity, sensitivity, DOR, PLR, and NLR based on the mean age of patients (≤52 or >52 years old), saliva type (stimulated and unstimulated saliva), biomarker measurement method (mass spectrometry [MS] and non‐MS measurement methods), sample size (≤55 or >55), biomarker type (proteomics, metabolomics, transcriptomics and proteomics, and reagent‐free biophotonic), and nations. Conclusion Saliva, as a noninvasive biomarker, has the potential to accurately differentiate breast cancer patients from healthy controls.

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Diagnostic Value of Salivary miRNA in Head and Neck Squamous Cell Cancer: Systematic Review and Meta-Analysis

Cited by 35 publications

References 38 publications

Salivary MicroRNA Signature for Diagnosis of Endometriosis

Salivary MicroRNA Signature for Diagnosis of Endometriosis

Human Papillomaviruses-Associated Cancers: An Update of Current Knowledge

Salivary biomarkers in breast cancer diagnosis: A systematic review and diagnostic meta‐analysis

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