Diagnostics in space: will zero gravity add weight to new advances?

Wong, Season

doi:10.1080/14737159.2020.1699061

Cited by 9 publications

(17 citation statements)

References 17 publications

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“…Until now, RT-qPCR testing was considered as the golden method for screening cases of COVID-19 and also chikungunya, dengue and Zika [ 21 ]. However, in a pandemic context, where a large number of analyses are required in a short time, these tests cannot be cost-effective.…”

Section: Integrated Analytical Platform For Fast and Cost-effective Of Eid Diagnosismentioning

confidence: 99%

“…Alternatively, IR spectroscopy and MALDI-TOF MS have been introduced as routine tools in microbiology laboratories, being used for identification of bacteria, fungi, and viruses. The usability of these techniques can cover identification of transmitting vector, pathogen agent, and also the virus detection in final receptor [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control

2021

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COVID-19 and arboviruses (ARBOD) epidemics co-occurrence is a great concern. In tropical and subtropical regions, ARBOD diseases such as chikungunya, dengue, and Zika are frequent. In both COVID-19 and ARBOD cases, an accurate diagnosis of infected patients is crucial to promote adequate treatment and isolation measures in COVID-19 cases. Overlap of clinical symptoms and laboratory parameters between COVID-19 and ARBOD present themselves as an extra challenge during diagnosis. COVID-19 diagnosis is mainly performed by quantitative reverse polymerase chain reaction (RT-qPCR), while ARBOD diagnosis is performed by serology, detection of antigen or antibody, and molecular diagnosis. In this review, the epidemiologic profile of arboviruses and SARS-CoV-2 is analyzed, and potential risks of symptom overlap is addressed. The implementation of an analytical platform based on infrared (IR) spectroscopy, MALDI-TOF mass spectrometry, and RT-qPCR is discussed as an efficient strategy for a fast, robust, reliable, and cost-effective diagnosis system even during the co-occurrence of virus outbreaks. The spectral data of IR spectroscopy and MALDI-TOF MS obtained from COVID-19 infected and recovered patients can be used to build up an integrated spectral database. This approach can enable us to determine quickly the groups that have been exposed and have recovered from COVID-19 or ARBOD, avoiding misdiagnoses.

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Section: Integrated Analytical Platform For Fast and Cost-effective Of Eid Diagnosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control

2021

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“…Polymerase chain reaction (PCR)-based nucleic acid amplification tests (NAATs) for N. gonorrhoeae significantly improved both the sensitivity and specificity of gonorrhea diagnosis [ 22 ]. Currently, Io CT/NG ® by Binx Health and GenXpert CT/NG ® by Cepheid are the only FDA-approved NAATs for gonorrhea [ 23 , 24 ]. However, these tests require controlled and restricted laboratory environments for diagnostic evaluation, with results generally available in a few days and are expensive to perform in developing countries.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, POCTs based on immunological methods (i.e., immunodiagnostics) still need to overcome issues associated with poor sensitivity and specificity due to the antigenic variability of N. gonorrhoeae strains for the detection of gonorrhea infections while meeting the established criteria [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. These issues are also reflected, in part, in commercial N. gonorrhoeae antibodies, which show poor specificity or the failure to recognize their targets.…”

Section: Introductionmentioning

confidence: 99%

“…For example, the sensitivity for detecting N. gonorrhoeae -positive female specimens was 70%, 12.5%, and 60% using GC Check (PATH), ACON NG Duo tests (ACON Laboratories, San Diego, USA), and Biostar OIA GC (ThermoFisher Scientific, Waltham, USA) test kits in development, respectively, compared with the standard reference tests confirmed by NAAT or culture tests [ 28 , 30 , 31 ]. Furthermore, the confidence interval (CI) for identifying N. gonorrhoeae in clinical specimens ranged between 55% and 82%, 0 and 41.7%, and 46 and 74% for GC Check, ACON NG Duo tests, and Biostar OIA GC, respectively [ 24 ]. Consequently, FDA-approved immunodiagnostic tests such as POCTs and enzyme-linked immunosorbent assays (ELISAs) for gonorrhea are unavailable.…”

Section: Introductionmentioning

confidence: 99%

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Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

et al. 2021

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Gonorrhea is one of the most common, but still hidden and insidious, sexually transmitted diseases caused by Neisseria gonorrhoeae (gonococci). However, the diagnosis and treatment of gonorrhea are hampered by antigenic variability among gonococci, the lack of acquired immunity, and antimicrobial resistance. Further, strains resistant to cephalosporins, including ceftriaxone, the last line of defense, represent a growing threat, which prompted us to develop gonococci-specific diagnostic antibodies with broad-spectrum binding to gonococci strains to generate gonorrhea-detecting reagents. This study reports the identification of gonococci antibodies via bio-panning on gonococci cells using scFv-phage libraries. Reformatting the lead scFv-phage Clones 1 and 4 to a multivalent scFv1-Fc-scFv4 maxibody increased the sensitivity by up to 20-fold compared to the single scFv-Fc (maxibody) alone. Moreover, the multivalent maxibody showed broader cross-reactivity with clinical isolates and the ceftriaxone antibiotic-resistant World Health Organization (WHO) reference strain L. In contrast, the selected antibodies in the scFv-phage, maxibody, and multivalent maxibody did not bind to N. sicca, N. meningitides, and N. lactamica, suggesting the clinical and pharmaceutical diagnostic value of these selected antibodies for gonorrheal infections. The present study illustrates the advantages and potential application of multivalent maxibodies to develop rapid and sensitive diagnostic reagents for infectious diseases and cancer.

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Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications

et al. 2021

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Healthy plants are vital for successful, long-duration missions in space, as they provide the crew with life support, food production, and psychological benefits. The microorganisms that associate with plant tissues play a critical role in improving plant health and production. To that end, we developed a methodology to investigate the transcriptional activities of the microbiome of red romaine lettuce, a key salad crop that was grown under International Space Station (ISS)-like conditions. Microbial transcripts enriched from host–microbe total RNA were sequenced using the Oxford Nanopore MinION sequencing platform. Results show that this enrichment approach was highly reproducible and could be an effective approach for the on-site detection of microbial transcriptional activity. Our results demonstrate the feasibility of using metatranscriptomics of enriched microbial RNA as a potential method for on-site monitoring of the transcriptional activity of crop microbiomes, thereby helping to facilitate and maintain plant health for on-orbit space food production.

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Diagnostics in space: will zero gravity add weight to new advances?

Cited by 9 publications

References 17 publications

COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control

COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control

Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications

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