Diagnostics of Pigmented Skin Tumors Based on Light-Induced Autofluorescence and Diffuse Reflectance Spectroscopy

Borisova, Ekaterina; Troyanova, P.

doi:10.1007/978-3-030-44594-2_7

Cited by 1 publication

(1 citation statement)

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“…Their key features of non-invasiveness, atraumaticity, sensitivity, cost-efficiency and clinical transfer potential, make them in vivo diagnostic tools of primary interest, complementary to other clinical gold standard procedures such as histopathology. In vivo skin diffuse reflectance (DR) [9][10][11] and autofluorescence (AF) [4,5,[12][13][14] spectroscopies or the combination of both [15][16][17], and the use of multiple excitation wavelengths for a better diagnostic accuracy [18], have been widely investigated for their applicability to provide a complementary information about the structural and biochemical changes accompanying the development of pathologies in skin tissues. For example, bimodal analysis combining multiply excited AF and DR spectral characteristics [17,19] resulted in increased diagnostic accuracy compared to a single excitation-AF approach when discriminating three types of mice skin hyperplasia, or normal columnar epithelium vs high-grade cervical precancers [20].…”

Section: Introductionmentioning

confidence: 99%

Impact of optical clearing on ex vivo human skin optical properties characterized by spatially resolved multimodal spectroscopy

Amouroux

Khairallah

Bashkatov

et al. 2021

Journal of Biophotonics

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A spatially resolved multimodal spectroscopic device was used on a two-layered "hybrid" model made of ex vivo skin and fluorescent gel to investigate the effect of skin optical clearing on the depth sensitivity of optical spectroscopy. Time kinetics of fluorescence and diffuse reflectance spectra were acquired in four experimental conditions: with optical clearing agent (OCA) 1 made of polyethylene glycol 400 (PEG-400), propylene glycol and sucrose; with OCA 2 made of PEG-400 and dimethyl sulfoxide (DMSO); with saline solution as control and a "dry" condition. An increase in the gel fluorescence back reflected intensitywas measured after optical clearing. Effect of OCA 2 turned out to be stronger than that of OCA 1, possibly due to DMSO impact on the stratum corneum keratin conformation. Complementary experimental results showed increased light transmittance through the skin and confirmed that the improvement in the depth sensitivity of the multimodal spectroscopic approach is related not only to the dehydration and refractive indices matching due to optical clearing, but also to the mechanical compression of tissues caused by the application of the spectroscopic probe.

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