Diallyl trisulphide inhibits adipogenesis in 3T3-L1 adipocytes through lipogenesis, fatty acid transport, and fatty acid oxidation pathways

Chang, Wei-Hsiang; Wu, Chi Hao; Hsu, Chin Lin

doi:10.1016/j.jff.2015.05.002

Cited by 19 publications

(14 citation statements)

References 40 publications

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“…This phenomenon is not surprising, since cells have smaller lipid content due to inhibition of glucose uptake and lipid synthesis and thus there is no need for cells to induce the production of lipases to break down lipid. Normally, up-regulation of PGC-1b increases fatty acid oxidation in the mitochondrial pathway [46,47]. However, in this study when we measured the level of PGC-1b, there was no change in all experimental groups.…”

Section: Discussioncontrasting

confidence: 66%

Lipogenesis inhibition and adipogenesis regulation via PPARγ pathway in 3T3-L1 cells by Zingiber cassumunar Roxb. rhizome extracts

Wong-a-nan

Inthanon

Saiai

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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Zingiber cassumunar (ZC) is used by tribal people in northern Thailand in traditional remedies for antiobesity and in food recipes. Extracts from this plant have been studied for several pharmacological effects including anti-obesity, but with no clear evidence on cellular mechanism of activity. This study aim to investigate the lipolytic and anti-adipogenic activity of crude extracts from ZC on in vitro cultures of the mouse adipocyte cell-model, 3T3-L1. Dry rhizome powder was extracted with absolute ethanol and boiled-water. On the exposed pre-adipocytes to the extracts, cytotoxicity was not detected by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid content and glycerol release were assessed using Oil Red-O and a commercial Adipolysis Assay kits respectively. The extracts exhibited no significant lipolytic activity on the exposed mature-adipocytes, in serial dilutions ranging from 1 to 800 mg/ml. However, anti-lipogenic activity was presented. All extracts significantly reduced the lipid content of exposed differentiating-adipocytes. This anti-lipogenic activity was confirmed by the expression of selected genes, determined by using real-time PCR techniques, in four groups namely: adipocyte differentiation genes, glucose uptake genes, lipid metabolism genes and fatty acid oxidation genes. 1 H NMR spectrum of the extracts exhibited the prominent olefinic protons of phenylbutanoids, the group of compounds previously proved with several bioactivities. This study provided evidences of mechanisms that apparently verify the traditional use of ZC to prevent obesity.

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Section: Discussioncontrasting

confidence: 66%

Lipogenesis inhibition and adipogenesis regulation via PPARγ pathway in 3T3-L1 cells by Zingiber cassumunar Roxb. rhizome extracts

Wong-a-nan

Inthanon

Saiai

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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“…Palmitic acid also represents an inflammatory mediator in the adipose tissue inducing white adipose tissue expansion and increasing inflammation through oxidative stress (Kennedy et al ., 2009), palmitic acid being found in high levels in digested donkey milk. On the other hand, many natural lipid compounds with anti-inflammatory and antioxidant effects have been used to treat obesity such as n-3 PUFA, EPA and DHA, MUFA and CLA (Kusunoki et al ., 2013; Chang et al ., 2015). Here we observed higher free oleic and linoleic acid levels in digested human and formula milk; oleic acid 1.5- and 6-folds higher and linoleic acid 2- and 5.5-folds higher than that found in milk from ruminant species, respectively.…”

Section: Resultsmentioning

confidence: 99%

Effect of lipid fraction of digested milk from different sources in mature 3T3-L1 adipocyte

Santillo

Figliola

Caroprese

et al. 2019

Journal of Dairy Research

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We evaluated the effect of in vitro digested milk on mature adipocytes 3T3-L1, paying particular attention to its fatty acid composition, and comparing human (HM), donkey (DM), bovine (BM), ovine (OM), caprine (CM) and formula (FM) milk. Cellular viability, apoptosis, oxidative response and gene expression levels of NF-κB p65, HMGB1, SREBP-1c and FAS were evaluated. Digested milk treatments significantly reduced 3T3-L1 mature adipocytes viability and caspase activity compared with control group, but no significant differences were observed among different sources of digested milk. In all digested milk samples, ROS level was higher than the control, however, the digested human and formula milk showed lower levels of ROS than DM, BM, OM and CM samples. Lower capacity of HM and FM to induce oxidative stress in mature adipocytes was ascribed to the peculiar free fatty acids profile of digested milk samples. All milk treatments elicited a significant over-expression of NF-κB p65 in 3T3-L1 adipocytes compared to the control; the lowest gene expression was found in HM, BM, OM and CM, the highest in FM and an intermediate behavior was shown in DM. All digested milk treatments influenced the gene expression of SRBP-1c with FM and HM showing the highest levels. For FAS expression, BM showed the highest level, OM and CM intermediate and FM, HM and DM the lowest levels, however HM and DM had comparable levels to the control.

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“…These compounds have recently received much interest in the search for natural bioactive compounds that contribute to anti-obesity properties. For example, diallyl trisulphide, a garlic-derived organo-sulphur compound, inhibited adipogenesis in 3T3-L1 adipocytes through regulating the gene levels of lipogenesis, lipolysis and adipokines [ 28 ]. Phillyrin prevents lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through LKB1/AMPK activation [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The Inhibitory Effects of Djulis (Chenopodium formosanum) and Its Bioactive Compounds on Adipogenesis in 3T3-L1 Adipocytes

et al. 2018

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The aim of this study was to provide new insights into the role of the ethanolic extracts of Djulis (Chenopodium formosanum, EECF) and its bioactive compounds in preventing adipogenesis in 3T3-L1 adipocytes. The results demonstrated EECF significantly inhibited oil red O-stained material (OROSM), triglyceride levels and glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 adipocytes. The expression of the critical molecules involved in lipid synthesis such as PPARγ, C/EBPα and SREBP-1c was attenuated in EECF-treated cells. According to HPLC-DAD and HPLC-MS/MS analysis, rutin, kaempferol, betanin and another nine compounds were present in EECF. The suppression of lipid accumulation by rutin, kaempferol and betanin occurred by decreasing the gene expression of PPARγ, C/EBPα and SREBP-1c. Taken together, these findings suggest the presence of bioactive compounds in EECF may partly account for the anti-adipogenesis of EECF and EECF is therefore a potentially lipid lowering functional food.

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Diallyl trisulphide inhibits adipogenesis in 3T3-L1 adipocytes through lipogenesis, fatty acid transport, and fatty acid oxidation pathways

Cited by 19 publications

References 40 publications

Lipogenesis inhibition and adipogenesis regulation via PPARγ pathway in 3T3-L1 cells by Zingiber cassumunar Roxb. rhizome extracts

Lipogenesis inhibition and adipogenesis regulation via PPARγ pathway in 3T3-L1 cells by Zingiber cassumunar Roxb. rhizome extracts

Effect of lipid fraction of digested milk from different sources in mature 3T3-L1 adipocyte

The Inhibitory Effects of Djulis (Chenopodium formosanum) and Its Bioactive Compounds on Adipogenesis in 3T3-L1 Adipocytes

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