Diamond Foods, Inc.

Sankara, Jomo; Lindberg, Deborah L.

doi:10.4135/9781526426499

Cited by 2 publications

(3 citation statements)

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“…Although Impossible Foods Inc. previously produced S‐Hb by overexpressing the transcriptional activator Mxr1 for P AOX1 in P. pastoris , [ 23 ] Mxr1 may not be the optimal choice among the three activators (Mit1, Mxr1, and Prm1). In this study, we investigated the contributions of these three activators to the synthesis of P‐Mb and found that Mit1 was the most effective, resulting in a 74.37% increase in P‐Mb titer when overexpressed (Figure 2c).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the main strategy to enhance heme supply in P. pastoris is to overexpress all genes involved in heme biosynthesis. [ 20,23 ] However, a recent study found that a significant accumulation of heme intermediates (PBG, HMB, CPG III, PPG IX, and PP IX) rather than heme was present in the fermentation products from an engineered P. pastoris strain that overexpressed all heme biosynthetic pathway genes through an analysis of ESI‐MS/MS. [ 38 ] These results indicated that this metabolic strategy was not suitable for hemoprotein production in P. pastoris .…”

Section: Discussionmentioning

confidence: 99%

“…737 and No. 1001) and applied by Impossible Foods Inc. [ 23 ] and Motif FoodWorks Inc. to develop the popular artificial meat products “Impossible Burgers” and “HEMAMI”, respectively. In addition, P. pastoris has an advantage over E. coli and S. cerevisiae in high‐cell‐density fermentation because it can grow on simple and inexpensive carbon sources to high densities.…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐SupplyPichia PastorisChassis

Yu,

Zhao,

Zhou

et al. 2023

Advanced Science

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Microbial synthesis of valuable hemoproteins has become a popular research topic, and Pichia pastoris is a versatile platform for the industrial production of recombinant proteins. However, the inadequate supply of heme limits the synthesis of high‐active hemoproteins. Here a strategy for enhancing intracellular heme biosynthesis to improve the titers and functional activities of hemoproteins is reported. After selecting a suitable expressional strategy for globins, the efficient heme‐supply P. pastoris chassis is established by removing the spatial segregation during heme biosynthesis, optimizing precursor synthesis, assembling rate‐limiting enzymes using protein scaffolds, and inhibiting heme degradation. This robust chassis produces several highly active hemoproteins, including porcine myoglobin, soy hemoglobin, Vitreoscilla hemoglobin, and P450‐BM3, which can be used in the development of artificial meat, high‐cell‐density fermentation, and whole‐cell catalytic synthesis of high‐value‐added compounds. Furthermore, the engineered chassis strain has great potential for producing and applying other hemoproteins with high activities in various fields.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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