Diapause induces remodeling of the fatty acid composition of membrane and storage lipids in overwintering larvae of Ostrinia nubilalis, Hubn. (Lepidoptera: Crambidae)

Vukašinović, Elvira L.; Pond, David W.; Worland, M. R.; Kojić, Danijela; Purać, Jelena; Popović, Željko D.; Grubor-Lajšić, Gordana

doi:10.1016/j.cbpb.2015.02.003

Cited by 23 publications

(14 citation statements)

References 38 publications

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“…Our findings suggest that a variable content of plant-derived lipids in different laboratory food recipes might account for these inconsistent results. Interestingly, phytophagous insects collected in the wild at different times of the year do show seasonal changes in fatty acid unsaturation (Pruitt and Lu, 2008;Rozsypal et al, 2014;Vuka sinovi c et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

A Temperature-Dependent Switch in Feeding Preference Improves Drosophila Development and Survival in the Cold

et al. 2018

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How cold-blooded animals acclimate to temperature and what determines the limits of their viable temperature range are not understood. Here, we show that Drosophila alter their dietary preference from yeast to plants when temperatures drop below 15 C and that the different lipids present in plants improve survival at low temperatures. We show that Drosophila require dietary unsaturated fatty acids present in plants to adjust membrane fluidity and maintain motor coordination. Feeding on plants extends lifespan and survival for many months at temperatures consistent with overwintering in temperate climates. Thus, physiological alterations caused by a temperature-dependent dietary shift could help Drosophila survive seasonal temperature changes.

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Section: Discussionmentioning

confidence: 99%

A Temperature-Dependent Switch in Feeding Preference Improves Drosophila Development and Survival in the Cold

et al. 2018

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“…The J III stage is considered environmentally resistant to freezing temperatures because it contains densely packed lipid droplets (Zhao et al, 2007). Like other nematodes and insects, the fatty acids of the PWN are mainly composed of palmitic acid, stearic acid, oleic acid and linoleic acid, but palmitoleic acid (C16:1) was not detected (Jagdale and Gordon, 1997; Vukašinović et al, 2015). The difference in the fatty acid profiles may be related to species and environmental factors (Thompson, 1973).…”

Section: Discussionmentioning

confidence: 97%

“…Overwintering individuals, such as Steinernema feltiae and other insects, have significantly greater ratios of unsaturated to saturated fatty acids (Sinclair and Marshall, 2018; Jagdale and Gordon, 1997). Unsaturated fatty acids with low melting points probably allow lipid membranes to maintain the sufficient fluidity required to maintain protein functions at low ambient temperatures (Nieminen et al, 2013; Vukašinović et al, 2015). The accumulations of low-molecular-weight sugars and polyhydric alcohols (polyols) are well-known responses to freezing, drought, salt and osmotic stresses in the entomopathogenic nematodes S .…”

Section: Introductionmentioning

confidence: 99%

Cold adaptive potential of pine wood nematodes overwintering in plant hosts

Liu

Pan

et al. 2019

Biology Open

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The pine wood nematode (PWN; Bursaphelenchus xylophilus ) is the causal agent of pine wilt disease, which results in severe ecological and economic losses in coniferous forests. During overwintering, PWNs undergo morphological and physiological changes to adapt to low temperature environments. Here, the physiological changes of the PWN populations sampled in the summer and winter were compared to analyze the role of low temperatures in their response. The PWN overwinters as third-stage dispersal juveniles, which showed significantly greater survival rates than summer populations (propagative forms) at sub-zero temperatures. The major biochemical compounds in the populations were analyzed by gas chromatography. Eight dominant fatty acids, with stearic acid being the most important, were identified from PWN propagative stage and third-stage dispersal stage. Compared with the propagative stage, the dispersal stage showed significant increases in the fatty acid content and the proportion of unsaturated fatty acids. Three carbohydrates, trehalose, glycerol and glucose, were detected in the PWN. Compared with the summer population, the levels of trehalose and glycerol increased significantly, while glucose decreased, in the winter population. The modifications in fatty acid composition and cryoprotectant levels, as elements of its changing physiology, play important roles in the overwintering success of the PWN.

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“…Diapause therefore plays a significant role in the population development and reproduction of insects (Clark & Worland, 2008;Hahn & Denlinger, 2011) as well as the stabilization of genetic characteristics during evolution (Denlinger, 2003). Characteristic changes of diapause process are lower metabolic activity and a pause in morphogenesis (Vukasinovic et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Expression analysis and functional identification of several genes related to diapause in Bombyx mori

et al. 2019

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Diapause is an important characteristic of insects used to adapt to extreme changes in environmental conditions. Embryonic diapause of the bivoltine silkworm (Bombyx mori) is determined by environmental conditions experienced by the mother while in the embryo stage. If they are incubated at 25°C with natural light, their progenies will be diapause‐destined. If they are incubated at 17°C in darkness, their progenies will be non‐diapause‐destined. The molecular mechanism of diapause remains unknown. In the present study, we analyzed two downregulated genes (BGIBMGA003835, BGIBMGA012335) and two upregulated genes (BGIBMG012996, BGIBMG002426) related to carbohydrate metabolism, verified differentially expressed in ovaries and heads of 1‐day‐old fifth‐instar larvae to 6‐day pupae by quantitative real‐time reverse transcription polymerase chain reaction (qRT‐PCR). In line with published data, the expression level of these genes in larvae were generally lower than in pupae. We further analyzed the expression levels of the four genes in BmN cells that had been treated with various concentrations of diapause hormone (DH). It demonstrated that the expression of these genes was affected by DH. Knockdown of the selected genes in non‐diapause‐destined female pupae changed the fate of the progeny from non‐diapause‐ to daipause‐destined, as seen by the appearance of diapause eggs. Our study provides insight into the molecular mechanism of diapause in B. mori.

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Diapause induces remodeling of the fatty acid composition of membrane and storage lipids in overwintering larvae of Ostrinia nubilalis, Hubn. (Lepidoptera: Crambidae)

Cited by 23 publications

References 38 publications

A Temperature-Dependent Switch in Feeding Preference Improves Drosophila Development and Survival in the Cold

A Temperature-Dependent Switch in Feeding Preference Improves Drosophila Development and Survival in the Cold

Cold adaptive potential of pine wood nematodes overwintering in plant hosts

Expression analysis and functional identification of several genes related to diapause in Bombyx mori

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