Diarrhea Caused in Gnotobiotic Piglets by the Reovirus-Like Agent of Human Infantile Gastroenteritis

Abstract

One- to four-day-old gnotobiotic piglets were inoculated orally with a reovirus-like agent obtained from human infants with acute gastroenteritis. Diarrhea developed in the piglets two to seven days after inoculation and was reproduced for five serial passages in one sequence and for three passages in another. Nineteen of 21 inoculated piglets developed diarrhea; reovirus-like particles were observed in intestinal contents and/or fecal samples from 17 animals with illness and from two inoculated piglets that did not develop diarrhea. One piglet, for which daily fecal samples were examined by electron microscopy, shed the largest number of virus particles at the onset of diarrhea. Immunofluorescent antibody responses to the reovirus-like agent were detected in sera from the seven inoculated animals that were tested.

Human rotavirus enteritis induced in conventional piglets. Intestinal structure and transport.

“…All animals in the infected group were diseased, but perhaps not all were at the same stage of infection when studied 72 h after they had received the virus. The variations in symptoms in our animals accord with the reported variability of the response of piglets to HRV infection (11,26,27). In part, these apparent variations may be due to the variable distribution and extent of the infection in the gut.…”

Intestinal Lesions Induced in Gnotobiotic Calves by the Virus of Human Infantile Gastroenteritis

“…The intestine from calf 2 was examined by scanning electron microscopy. Equipment, procedures and isolation units have been described [13].Source and early calf passages of the D strain of the viral agent have been described [8,19,201. The calves were inoculated orally 114) at 8-10 hours old when they were first fed milk.…”

“…Immunofluorescence in the small intestinal lamina propria was not evaluated because of nonspecific staining of eosinophils. Indirect-immunofluorescence [ 19,201 on sections of distal small intestine from calf 3 was done with acute and convalescent-phase human infant serum and then by fluorescein-conjugated goat antiserum to human gamma-globulin (Antibodies, Inc., Davis, Calif.). Intestine and mesenteric lymph nodes for transmission electron microscopy were prepared as described (171.…”

Patterns of Shedding of Human Reovirus-Like Agent in Gnotobiotic Newborn Piglets with Experimentally-Induced Diarrhea

“…Investigations recently have been directed toward finding susceptible animal species to be used as animal models [6][7][8][9]. Such studies have revealed that the human reovirus-like (HRVL) agent may induce diarrhea in piglets, monkeys, and calves [6,7,9,10].…”