Diarrheal Diseases in Children and Adults Associated with &lt;i&gt;Escherichia coli&lt;/i&gt; Strains not Belonging to Enteropathogenic Serotypes

Costin, I. D.

doi:10.1159/000161903

Cited by 5 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, illness caused by infection with noninvasive EEC strains in children and adults, as well as in infants, is usually gastroenteritis or occasionally enteritis or colitis, resembling salmonellosis (Costin, 1966;Sakazaki et al, 1967.…”

Section: Enteropathogenicitymentioning

confidence: 99%

Enteropathogenicity and Enterotoxigenicity of Human Enteropathogenic Escherichia Coli

Sakazaki¹,

Tamura²,

Nakamura³

et al. 1974

JJMSB

View full text Add to dashboard Cite

SUMMARY :The mechanism of enteropathogenicity of human enteropathogenic Escherichia coli (EEC) was studied by ligated gut loop tests in rabbits, keratoconjunctival tests in guinea pig eyes and feeding tests on human volunteers, and by examining for their ability to penetrate into HeLa cells.Strains of invasive EEC (Shigella-like) serotypes produced inflammatory changes and fluid accumulation in the ligated rabbit gut loop, keratoconjunctivitis in guinea pig eyes, and penetration into HeLa cells. With few exceptions, no enterotoxic activity was demonstrated with cell-free filtrates of broth cultures. It was considered that EEC strains of this group may probably cause disease in man by a mechanism similar to that of Shigella organisms, and that enterotoxin production may not be concerned with the pathogenicity of these organisms.When strains of noninvasive EEC including 27 OK group serotypes were studied, 46 of 67 cultures employed produced fluid accumulation and histopathological changes in ligated gut loop tests in rabbits, whereas none of them induced keratoconjunctivitis in guinea pig eyes nor penetration into HeLa cells. In addition, cell-free filtrates from broth cultures of 12 out of 20 strains produced alterations similar to those caused by living culture in the gut loop tests. When R-state cultures derived from three strains of noninvasive EEC serotypes were tested for their activity in rabbit gut loops, it was demonstrated that the mutants no longer had the ability to produce positive gut loop reactions, although filtrates of the R cultures were still able to cause pathological alterations in the loops. Cell cultures killed by acetone, chloroform, or heat failed to give positive gut loop reactions.Positive gut loop reactions were also induced by some strains not belonging to the recognized EEC serotypes originating from sources other than gastroenteritis patients.Nine strains consisting of four of noninvasive EEC and five enterotoxigenic strains not belonging to the recognized EEC serotypes were tested with human volunteers.All the former strains caused acute gastroenteritis in the volunteers, while the latter elicited on response.These results suggest that enterotoxin production is not concerned in the mechanism of enteropathogenicity of noninvasive EEC.On the other hand, it seemed that the ability to multiply in the intestine is essential in the enteropathogenicity of noninvasive EEC strains, although an enterotoxic substance demonstrated in cell-free filtrates may play a role in the development of gastroenteritis.

show abstract

Section: Enteropathogenicitymentioning

confidence: 99%

Enteropathogenicity and Enterotoxigenicity of Human Enteropathogenic Escherichia Coli

Sakazaki¹,

Tamura²,

Nakamura³

et al. 1974

JJMSB

View full text Add to dashboard Cite

show abstract

Sensitivity of strains of enteropathogenicEscherichia coli to tobramycin and other antibiotics

Konforti¹,

Lev²,

Hanner³

1977

Med Microbiol Immunol

View full text Add to dashboard Cite

The sensitivity patterns of strains of enteropathogenic Escherichia coli associated with diarrhoeal disease of infants to tobramycin and other antibiotics were estimated. The activity of tobramycin and of gentamicin alone and in combinations against recent isolates of enteropathogenic Escherichia coli was investigated. It was found that all the strains included in the present study were sensitive to tobramycin and gentamicin and most of them were sensitive to colistin and furazolidone. No significant differences in minimal inhibitory concentrations (MICs) and rates of killing were found between tobramycin and gentamicin, and they acted in an additive manner against most of the strains tested.

show abstract

Enteropathogenic Escherichia coli: Lack of Correlation of Serotype with Pathogenicity

Goldschmidt¹,

DuPont²

1976

Journal of Infectious Diseases

View full text Add to dashboard Cite

Forty-eight strains of Escherichia coli isolated from children with diarrhea were classified according to nine enteropathogenic serotypes. The strains were examined for production of enterotoxin and for invasiveness by study of bacteria or bacteria-free filtrates in conventional animal and tissue culture models. Filtrates of only three strains (6%) consistently dilated rabbit ileal loops, while all 48 strains yielded negative results in suckling mice, adrenal cells, and guinea pig eyes. When filtrates of the three strains that dilated the rabbit ileum were heated at 60 C for 30 min, the reaction in rabbit ileal loops was negative; this finding indicated the production of a heat-labile enterotoxin. This study shows the lack of correlation between classical enteropathogenic serotypes of E. coli and presently known virulence properties in animal models. The results raise doubts about the value of serotyping E. coli isolates from sporadic cases of diarrhea. When it is suspected that an E. coli isolate is enteropathogenic, it may be important to perform more than one laboratory assay.

show abstract

Diarrheal Diseases in Children and Adults Associated with <i>Escherichia coli</i> Strains not Belonging to Enteropathogenic Serotypes

Cited by 5 publications

References 5 publications

Enteropathogenicity and Enterotoxigenicity of Human Enteropathogenic Escherichia Coli

Enteropathogenicity and Enterotoxigenicity of Human Enteropathogenic Escherichia Coli

Sensitivity of strains of enteropathogenicEscherichia coli to tobramycin and other antibiotics

Enteropathogenic Escherichia coli: Lack of Correlation of Serotype with Pathogenicity

Contact Info

Product

Resources

About