Diarylheptanoids from <i>Alpinia officinarum</i> Cause Distinct but Overlapping Effects on the Translatome of B Lymphoblastoid Cells

Kakegawa, Tomohito; Takase, Saeko; Masubuchi, Eri; Yasukawa, Ken

doi:10.1155/2014/204797

Cited by 13 publications

(12 citation statements)

References 27 publications

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“…Although the present direct translatome comparisons of alpinone 3-acetate and hydrocortisone indicate greater effects of hydrocortisone in TPAinduced cells, comparisons in WiKiPathways (Table 4) were not sufficient to ascribe the details of bioactive differences between these compounds with positive Z scores in WiKiPathways for both agents. In contrast, our previous experiments with Alpinia diarylheptanoids resulted in positive and negative Z scores [16]. Hence, alpinone 3-acetate and particularly hydrocortisone may downregulate genes in translatome analyses to a lesser extent than diarylheptanoids.…”

Section: Discussioncontrasting

confidence: 78%

“…3). Moreover, nine and five gene probes were differentially upregulated and downregulated by alpinone Microarray and data analyses were performed as previously described [16] by the contract analysis service at Filgen Incorporation Japan. Gene expression was considered significantly changed after calculating ratio flags, which indicate upregulation or downregulation (ratio C2 or B0.5).…”

Section: Discussionmentioning

confidence: 99%

“…Polysome analyses were performed as described previously [16] with slight modifications. Briefly, 60-ml suspensions of exponentially growing THP-1 cells (0.5 9 10 6 cells/ml) were collected by centrifugation, resuspended in 30 ml of medium containing 100-nM TPA, and cultured in 150-cm 2 plates for 24 h, resulting in differentiation into macrophages.…”

Section: Cellular Fractionation and Rna Preparationmentioning

confidence: 99%

“…2, and heavy fractions (#6, 7, and 8; Fig. 2a) were stored at -30°C until total RNA extraction [16].…”

Section: Cellular Fractionation and Rna Preparationmentioning

confidence: 99%

“…We isolated noble diarylheptanoid compounds from Alpinia officinarum [8,9] and demonstrated anti-tumor [9][10][11], anti-inflammatory [10], anti-influenza viral [12,13], and anti-RS virus properties [14,15]. In a subsequent study, we reported using translatome analyses to investigate the mechanisms and modes of action of these compounds [16]. In these experiments, human B lymphoblastoid cells were exposed to diarylheptanoids and were used to prepare pools of ribosome-associated mRNAs for DNA microarray hybridization analyses.…”

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds

2016

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We aimed to investigate the bioactive components of Alpinia japonica as anti-inflammatory compounds using searches of the Alpinia genus, and subsequently demonstrated that alpinone 3-acetate markedly inhibits 12-O-tetradecanoyiphorbol 13-acetate-induced inflammation in a mouse model of ear edema. To assess other bioactivities of alpinone 3-acetate, we performed translatome analyses and compared them with those of hydrocortisone. Polysome-associated mRNAs were prepared from alpinone 3-acetate- or hydrocortisone-treated and control cells from 12-O-tetradecanoyiphorbol 13-acetate-induced THP-1-derived macrophages cultured in the presence of Escherichia coli O-111 lipopolysaccharide. Subsequent microarray analysis revealed that alpinone 3-acetate and hydrocortisone upregulated and downregulated the same 155 and 41 genes, respectively. Moreover, direct comparisons of translationally regulated genes indicated 5 and 10 gene probes that were upregulated and downregulated by alpinone 3-acetate and hydrocortisone, respectively. In conclusion, assays of 12-O-tetradecanoyiphorbol 13-acetate-induced inflammation ear edema in mice and polysome profiling of alpinone 3-acetate bioactivities indicated similar medicinal possibilities to those of hydrocortisone.

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Section: Discussioncontrasting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Cellular Fractionation and Rna Preparationmentioning

confidence: 99%

“…2, and heavy fractions (#6, 7, and 8; Fig. 2a) were stored at -30°C until total RNA extraction [16].…”

Section: Cellular Fractionation and Rna Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds

2016

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Extraction and isolation of diphenylheptanes and flavonoids fromAlpinia officinarumHance using supercritical fluid extraction followed by supercritical fluid chromatography

Dai

et al. 2023

J of Separation Science

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In this paper, an off-line combination method of supercritical fluid extraction and supercritical fluid chromatography was developed for the selective extraction and isolation of diphenylheptanes and flavonoids from Alpinia officinarum Hance.The enrichment of target components was successfully achieved using supercritical fluid extraction with the following conditions (8% ethanol as co-solvent at 45 • C and 30 MPa for 30 min). Taking full advantage of the complementarity of supercritical fluid chromatography stationary phases, a two-step preparative supercritical fluid chromatography strategy was constructed. The extract was firstly divided into seven fractions on a Diol column (250 × 20 mm internal diameter, 10 μm) within 8 min by gradient elution increasing from 5% to 20% modifier (methanol) at 55 ml/min and 15 MPa. Then the seven fractions were separated by using a 1-AA or a DEA column (250 × 19 mm internal diameter, 5 μm) at 50 ml/min and 13.5 MPa. This two-step strategy showed superior separation ability for structural analogs. As a result, seven compounds, including four diphenylheptanes and three flavonoids with high purity, were successfully obtained. The developed method is also helpful for the extraction and isolation of other structural analogs of traditional Chinese medicines.

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Shikonin changes the lipopolysaccharide-induced expression of inflammation-related genes in macrophages

et al. 2017

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We aimed to find candidate molecules possibly involved in the anti-inflammatory activity of shikonin (active compound of "Shikon") by analyzing its effects on gene expression of lipopolysaccharide (LPS)-treated THP-1 macrophages. Polysome-associated mRNAs (those expected to be under translation: translatome) from cells treated with LPS alone (LPS: 5 µg/mL), shikonin alone (S: 100 nM), or LPS plus shikonin (LPS&S) for 3 h were analyzed by DNA microarray followed by detection of enriched pathways/gene ontologies using the tools of the STRING database. Candidate genes in enriched pathways in the comparison of LPS&S cells vs. LPS cells were analyzed by reverse-transcription quantitative real-time PCR (RT-qPCR; 1, 2, and 3 h). DNA microarray showed shikonin significantly influences gene expression. Gene expression changes between LPS&S cells and LPS cells were compared to detect relevant proteins and/or mRNAs underlying its anti-inflammatory effects: shikonin downregulated pathways which were upregulated in LPS cells, for example, 'innate immune response'. Within changed pathways, three genes were selected for RT-qPCR analyses as key candidates influencing inflammatory responses: CYBA (component of the superoxide-generating Nox2 enzyme), GSK3B (controller of cell responses after toll-like receptor stimulation), and EIF4E (a key factor of the eukaryotic translation initiation factor 4F complex that regulates abundance of other proteins involved in immune functions). All three mRNAs were decreased at 2 h, and CYBA continued low at 3 h relative to LPS cells. Given that shikonin decreased the expression of CYBA gene of Nox2, in addition to the direct inhibition of the Nox2 activity that we have previously shown, it is suggested that one of its anti-inflammatory mechanisms could be attenuation of oxidative stress.

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Diarylheptanoids from Alpinia officinarum Cause Distinct but Overlapping Effects on the Translatome of B Lymphoblastoid Cells

Cited by 13 publications

References 27 publications

Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds

Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds

Extraction and isolation of diphenylheptanes and flavonoids fromAlpinia officinarumHance using supercritical fluid extraction followed by supercritical fluid chromatography

Shikonin changes the lipopolysaccharide-induced expression of inflammation-related genes in macrophages

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