Diatom-Inspired Bionic Hydrophilic Polysaccharide Adhesive for Rapid Sealing Hemostasis

Abstract

Diatoms are typical marine biofouling organisms that secrete extracellular polymers (EPS) to achieve strong underwater adhesion. Here, we report a diatom-inspired bionic hydrophilic polysaccharide adhesive composed of diatom biosilica (DB) and bletilla striata polysaccharide (BSP) for rapid sealing hemostasis. The hierarchical porous structure of DB with rich surface silanol groups provides a strong anchored interface effect for BSP, which can significantly enhance cross-linking density and interaction strength of the hydrophilic macromolecular network. BSP/DB adhesive offers 6 times greater mechanical strength and viscosity over BSP under different temperature conditions. The aggregation effect of DBs interface for BSP avoided the washout of BSP/DB adhesive during application in a wet environment before cross-linking occurs. This strengthened the adhesion ability of BSP/DB adhesive to biological tissue that brought out complete sealing hemostasis without blood loss in a rat liver injury model. The dry BSP/DB prepared by lyophilization inherited excellent clotting ability of BSP/DB adhesive, which could realize rapidly the cruor of anticoagulant whole blood within 1 min. The results of animal studies confirmed that dry BSP/DB exhibited superior hemostatic performance over silicate-based inorganic Quikclot, in terms of hemostatic rate, blood loss, dosage, and multiscroll wound closure.

Degradable Nanohydroxyapatite-Reinforced Superglue for Rapid Bone Fixation and Promoted Osteogenesis

“…As shown in FT-IR, compared with BDSG without nHAP, the -OH (3411 cm –1 ) and CO (1714 cm –1 ) peaks of O-BDSG-OH (BDSG with nHAP) shift to a lower wavenumber, and the vibration band of -OH peak widens, while no corresponding change was observed in O-BDSG-CH 3 (BDSG with nHAP-CH 3 ) (Figure F). This indicates the formation of hydrogen bonds between nHAP and polymer chains. , The area of two peaks of H-bonded -OH groups (∼3410 cm –1 ) and free -OH groups (∼3500 cm –1 ) was further separately determined by Gaussian fitting of the peaks . And the peak area ratio (peak area of 3410 cm –1 divided by the sum of the areas of peak 3500 cm –1 and peak 3410 cm –1 ) of O-BDSG-OH is higher than those of both BDSG and O-BDSG-CH 3 , also reflecting the existence of new hydrogen bonds (Figure S13).…”

Silk fibroin-based hemostatic powders with instant and robust adhesion performance for sutureless sealing of gastrointestinal defects

“…S4 (ESI†), the storage modulus ( G ′) was higher than the loss modulus ( G ′′) over the whole range of angular frequency, which is a characteristic of stable gel-like behavior. 37,38 Strain-dependent oscillatory rheology of the bulk hydrogel displayed broad linear viscoelastic regions with a gel to sol cross-over point at about 7% strain, reflecting a wide processing regime (Fig. S5, ESI†).…”

Progress in injectable hydrogels for the treatment of incompressible bleeding: an update

“…Due to the high temperature sensitivity of these substances, painless removal of the material can be performed by changing the ambient temperature (e.g., by introducing ice). The diatom biosilica, which contains a large number of surface silanol groups, was incorporated into bletilla striata polysaccharide, a traditional Chinese medicine ingredient with haemostatic effects ( Sun et al, 2023 ). The structural features of the diatom biosilica increased the cohesion of the material and the force applied to the tissue surface to improve the contact efficiency and duration of the material with the wound, reducing the bleeding volume from 452 mg to 250 mg and the bleeding time from 327 s to 186 s. Diatom biosilica was also used to reduce the bleeding time from 327 s to 186 s.…”