Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity

Coesel, Sacha; Mangogna, Manuela; Ishikawa, Tomoko; Heijde, Marc; Rogato, Alessandra; Finazzi, Guido; Todo, Tomoki; Bowler, Chris; Falciatore, Angela

doi:10.1038/embor.2009.59

Cited by 181 publications

(195 citation statements)

References 38 publications

Supporting

Mentioning

181

Contrasting

Unclassified

Order By: Relevance

“…Proteins were extracted as described in ref. 32. The diatom LHCX proteins (around 22 kDa) were detected using a rabbit polyclonal anti-LHCSR antibody from Chlamydomonas (gift of Graham Peers, University of California, Berkeley, CA), used at a dilution of 1:5,000 (referred to as α-LHCX in the figures).…”

Section: Methodsmentioning

confidence: 99%

An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to light

Bailleul

Rogato

Martino

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

277

283

View full text Add to dashboard Cite

Diatoms are prominent phytoplanktonic organisms that contribute around 40% of carbon assimilation in the oceans. They grow and perform optimally in variable environments, being able to cope with unpredictable changes in the amount and quality of light. The molecular mechanisms regulating diatom light responses are, however, still obscure. Using knockdown Phaeodactylum tricornutum transgenic lines, we reveal the key function of a member of the light-harvesting complex stress-related (LHCSR) protein family, denoted LHCX1, in modulation of excess light energy dissipation. In contrast to green algae, this gene is already maximally expressed in nonstressful light conditions and encodes a protein required for efficient light responses and growth. LHCX1 also influences natural variability in photoresponse, as evidenced in ecotypes isolated from different latitudes that display different LHCX1 protein levels. We conclude, therefore, that this gene plays a pivotal role in managing light responses in diatoms.

show abstract

Section: Methodsmentioning

confidence: 99%

An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to light

Bailleul

Rogato

Martino

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

277

283

View full text Add to dashboard Cite

show abstract

“…The animal-like CRY in Porphyra is closely affiliated with the cryptochrome photolyase family (e.g., Ostreococcus tauri, Phaeodactylum tricornutum in SI Appendix, Fig. S27), which has maintained photolyase activity (60,61), in contrast to other plant and animal-like CRYs. Moreover, cryptochrome photolyase family CRY can affect transcriptional activity in a heterologous clock system (e.g., mammalian CRY) and control blue-lightdependent cellular processes, as found with insect or plant CRYs (60,61).…”

Section: Significancementioning

confidence: 99%

Insights into the red algae and eukaryotic evolution from the genome of Porphyra umbilicalis (Bangiophyceae, Rhodophyta)

Brawley¹,

Blouin²,

Ficko-Blean³

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

238

222

View full text Add to dashboard Cite

Porphyra umbilicalis (laver) belongs to an ancient group of red algae (Bangiophyceae), is harvested for human food, and thrives in the harsh conditions of the upper intertidal zone. Here we present the 87.7-Mbp haploid Porphyra genome (65.8% G + C content, 13,125 gene loci) and elucidate traits that inform our understanding of the biology of red algae as one of the few multicellular eukaryotic lineages. Novel features of the Porphyra genome shared by other red algae relate to the cytoskeleton, calcium signaling, the cell cycle, and stress-tolerance mechanisms including photoprotection. Cytoskeletal motor proteins in Porphyra are restricted to a small set of kinesins that appear to be the only universal cytoskeletal motors within the red algae. Dynein motors are absent, and most red algae, including Porphyra, lack myosin. This surprisingly minimal cytoskeleton offers a potential explanation for why red algal cells and multicellular structures are more limited in size than in most multicellular lineages. Additional discoveries further relating to the stress tolerance of bangiophytes include ancestral enzymes for sulfation of the hydrophilic galactan-rich cell wall, evidence for mannan synthesis that originated before the divergence of green and red algae, and a high capacity for nutrient uptake. Our analyses provide a comprehensive understanding of the red algae, which are both commercially important and have played a major role in the evolution of other algal groups through secondary endosymbioses.cytoskeleton | calcium-signaling | carbohydrate-active enzymes | stress tolerance | vitamin B 12T he red algae are one of the founding groups of photosynthetic eukaryotes (Archaeplastida) and among the few multicellular lineages within Eukarya. A red algal plastid, acquired through secondary endosymbiosis, supports carbon fixation, fatty acid synthesis, and other metabolic needs in many other algal groups in ways that are consequential. For example, diatoms and haptophytes have strong biogeochemical effects; apicomplexans cause human disease (e.g., malaria); and dinoflagellates include both coral symbionts and toxin-producing "red tides" (1). The evolutionary processes that produced the Archaeplastida and secondary algal lineages remain under investigation (2-5), but it is clear that both nuclear and plastid genes from the ancestral red algae have contributed dramatically to broader eukaryotic evolution and diversity. Consequently, the imprint of red algal metabolism on the Earth's climate system, aquatic foodwebs, and

show abstract

“…87) In the diatom Phaeodactylum tricornutum, PtCPF1 (Phaeodactylum tricornutum cryptochrome/photolyase family 1) is a novel cryptochrome/photolyase family member that not only repairs UV-induced DNA damage but also acts as a transcriptional repressor of the circadian clock. 88) In addition, the critical role of redox signaling in the light-dependent entrainment of the circadian clock 57,68) strongly implicates cellular responses to the toxic effects of sunlight as the evolutionary origin of circadian rhythms.…”

Section: Perspectivementioning

confidence: 99%

A Common Origin: Signaling Similarities in the Regulation of the Circadian Clock and DNA Damage Responses

Uchida

Hirayama

Nishina

2010

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

INTRODUCTIONFrom bacteria to humans, almost all organisms can adapt the timing of their physiology to the cyclic changes of their environment, thanks to a naturally-selected intrinsic timekeeping system called the circadian clock. 1) The circadian clock enhances the physiological efficiency and survival of an organism by organizing its behavior and body functions. 2,3) During the circadian day, the organism's physiology is given over to catabolic processes, whereas the anabolic functions of growth, repair and consolidation occur at night. To achieve this schedule in mammals, the circadian clock regulates a number of physiological functions, including sleep and wakefulness, food intake, body temperature, cardiovascular and renal activity, hormone production, hepatic metabolism and immune responses. 4,5) Accordingly, disruption of the circadian clock in humans has been linked to profound effects on health, including insomnia, stomach ailments, depression and cancer. 4,5) In most organisms, the molecular mechanisms underlying the establishment and maintenance of biological rhythms comprise interconnected transcription-translation feedback loops in which some clock factors repress their own transcription once they have attained critical levels. 2,3) These oscillators have the property of being endogenous and cellautonomous systems that maintain their rhythm in the absence of external time cues. 6) Both vertebrates and invertebrates have circadian oscillators scattered throughout their bodies. 7,8) In mammals, the circadian system is composed of both central and peripheral oscillators. 7) The mammalian central clock is located in the suprachiasmatic nucleus (SCN) within the anterior hypothalamus of the brain. 9) This central clock acts as a coordinator and provides time signals via both neural and humoral routes that entrain independent peripheral clocks. Dysfunction of the central clock does not inactivate the peripheral clocks but instead causes individual peripheral oscillators to become temporally uncoupled. [10][11][12] To guarantee that an organism's behavior remains tied to the rhythms of its environment, the circadian clock must be able to reset itself in response to environmental cues. 9,13,14) The main environmental stimulus for organisms is light, which is provided in day-night cycles. Mammals have no photoreceptors in peripheral tissues, 15) so that the effect of light on peripheral clocks is indirect. 13) For the mammalian clock, the SCN integrates photic cues from the retina and uses neural and humoral signals to transmit this information to peripheral clocks, synchronizing them. [16][17][18] This communication between the central and peripheral clocks results in the seamless regulation of fundamental physiological functions. 4,8) Interestingly, peripheral clocks can also respond directly to SCN-independent signals such as feeding and temperature change. 19,20) However, the physiological role of SCN-independent responses of peripheral clocks is not yet fully understood. A recent study has reported that ...

show abstract

Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity

Cited by 181 publications

References 38 publications

An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to light

An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to light

Insights into the red algae and eukaryotic evolution from the genome of Porphyra umbilicalis (Bangiophyceae, Rhodophyta)

A Common Origin: Signaling Similarities in the Regulation of the Circadian Clock and DNA Damage Responses

Contact Info

Product

Resources

About