Dication and trication which can increase the permeability of Escherichia coli outer membrane

Katsu, Takashi; Shibata, Mie; Fujita, Yuzuru

doi:10.1016/0005-2736(85)90138-5

Cited by 15 publications

(21 citation statements)

References 30 publications

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“…Cells were grown in minimal salt medium, supplemented with 1% Polypeptone, at 37°C under aerobic conditions (14). Cells were harvested during the exponential phase of growth, washed twice with buffer (100 mM choline chloride and 50 mM MOPS-Tris, pH 7.2), and suspended in the same buffer at 12 mg of cell protein/ml (8,10,(12)(13)(14)(15)(16). The protein content was determined by the method of Lowry et al (18).…”

Section: Methodsmentioning

confidence: 99%

“…Apparently, the binding of the peptide to LPS was not enough to permeabilize the outer membrane, indicating that other factors are involved. On the other hand, much simpler polyamines than PMBN have been reported to increase the permeability of the outer membrane (8,13). These polyamines possess two or three amino groups, which were further modified with bulky hydrophobic moieties (8,13).…”

mentioning

confidence: 99%

“…On the other hand, much simpler polyamines than PMBN have been reported to increase the permeability of the outer membrane (8,13). These polyamines possess two or three amino groups, which were further modified with bulky hydrophobic moieties (8,13). Thus, PMBN is not the only compound to increase the permeability of the outer membrane.…”

mentioning

confidence: 99%

“…1. Potentiometric measurements made with ion-selective electrodes were used, because the measurement process is fast, easy, and continuous in turbid cell suspensions (3,7,8,10,11,(13)(14)(15)(16)26). In this study, we used Ca 2ϩ , tetraphenylphosphonium (TPP ϩ ), and K ϩ electrodes.…”

mentioning

confidence: 99%

“…This approach seemed to be especially effective for analyzing the action of cationic compounds on the outer membrane, since these compounds are considered to expel divalent cations (such as Ca 2ϩ and Mg 2ϩ ) stabilizing LPS (14,15). The TPP ϩ electrode was used to monitor the increase in permeability of the outer membrane, because the uptake of TPP ϩ , which was stimulated by the membrane potential (inside negative) formed in the cytoplasmic membrane, was promoted when the permeability barrier of the outer membrane was disrupted (3,8,(13)(14)(15). The K ϩ electrode was used to monitor the increase in permeability of the cytoplasmic membrane, which usually leads to dissipation of membrane potential, resulting in the efflux of the accumulated TPP ϩ (10, 16).…”

mentioning

confidence: 99%

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Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

Katsu

Nakagawa

Yasuda

2002

Antimicrob Agents Chemother

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We analyzed the interaction between polyamines and the outer membrane of Escherichia coli cells using potentiometric measurements with Ca 2؉ , tetraphenylphosphonium (TPP ؉ ), and K ؉ electrodes. The Ca 2؉electrode was used to examine the ability of the polyamines to release Ca 2؉ from the outer membrane. The TPP ؉ electrode was used to examine the ability to permeabilize the outer membrane, since the uptake of TPP

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

Katsu

Nakagawa

Yasuda

2002

Antimicrob Agents Chemother

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Compromising the Protective Barrier of the Gram-Negative Bacterial Cell Surface

Reese¹,

Clancy²

1992

Emerging Targets in Antibacterial and Antifungal Chemotherapy

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Effect of histamine on the signal transduction of the AtoS–AtoC two component system and involvement in poly-(R)-3-hydroxybutyrate biosynthesis in Escherichia coli

et al. 2008

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AtoS-AtoC two-component system acts directly on the atoDAEB operon transcription to regulate the biosynthesis of short-chain poly-(R)-3-hydroxybutyrate. This study sought to investigate the effect of histamine and compound 48/80 on the regulation of AtoS-AtoC two-component system in Escherichia coli K-12 MA255 (speC(-), speB(-)) and the isogenic E. coli strains BW25113 (atoSC(+)) and BW28878 (DeltaatoSC) transformed with plasmids carrying related genes. Histamine or compound 48/80 induced or tended to reduce atoC transcription, respectively, while neither compound showed any effect on atoDAEB operon transcription. Moreover, histamine down-regulated poly-(R)-3-hydroxybutyrate biosynthesis, whereas compound 48/80 up-regulated its biosynthesis, maximal induction being obtained in the presence of multiple copies of AtoS-AtoC. Interestingly, co-administration of histamine counteracted this inductive effect of compound 48/80. The reported data provide the first evidence for a differential modulator role of histamine and compound 48/80 on the AtoS-AtoC two-component system signaling in potentially pathogenic bacteria, leading to a new perspective on their symbiotic behavior.

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Dication and trication which can increase the permeability of Escherichia coli outer membrane

Cited by 15 publications

References 30 publications

Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

Compromising the Protective Barrier of the Gram-Negative Bacterial Cell Surface

Effect of histamine on the signal transduction of the AtoS–AtoC two component system and involvement in poly-(R)-3-hydroxybutyrate biosynthesis in Escherichia coli

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