Dicentric and translocation analysis for retrospective dose estimation in humans exposed to ionising radiation during the Chernobyl nuclear power plant accident

Salassidis, K.; Schmid, E.; Peter, R. U.; Braselmann, Herbert; Bauchinger, M.

doi:10.1016/0027-5107(94)90071-x

Cited by 74 publications

(23 citation statements)

References 10 publications

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“…Several investigations using conventional cytogenetic methods, as well as fluorescence in situ hybridization (FISH) techniques, were carried on the populace in these countries in order to evaluate the presence of structural chromosome aber rations in circulating lymphocytes and to attempt an estimate of the absorbed dose. The results of these studies were in good agreement, showing an elevated incidence of chromosomal breakage in exposed subjects (Stephan and Oestreicher, 1989;Pohl-Ruling et al, 1991;Verschaeve et al, 1993;Salassidis et al, 1994Salassidis et al, , 1995Granath et al, 1996a;Snigiryova et al, 1997). In addition, a significant increase in cancer incidence was detected in subjects having a high frequency of chromosome aberrations in somatic cells; as a consequence, the observed DNA damage may be considered an early indicator of cancer risk (The Nordic Study Group, 1990;Bonassi et al, 1995).…”

supporting

confidence: 74%

FISH analysis of translocations in lymphocytes of children exposed to the Chernobyl fallout: preferential involvement of chromosome 10

Scarpato

Lori

Panasiuk

et al. 1997

Cytogenet Genome Res

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Using whole-chromosome painting probes, we have analyzed the frequency of translocations of chromosomes 1, 3, and 10 in peripheral lymphocytes of 20 Gomel (Belarus) children, including both thyroid tumor affected and healthy individuals. Gomel was one of the most heavily radiocontaminated areas due to fallout from the Chernobyl nuclear power plant disaster. As controls, 14 healthy children from Pisa (Italy) were investigated simultaneously. Translocation rates were significantly higher in the tumor affected (1.71 ± 0.68) and healthy Gomel children (2.69 ± 0.50) than in the Italian controls (0.79 ± 0.24). We also observed, in healthy Gomel children, an approximately three-fold higher frequency of chromosome 10 translocations compared to translocations affecting chromosomes 1 or 3 (P = 0.0096), a difference that was made even larger (about four fold) after correcting for chromosome size (P = 0.0009). This finding suggests a preferential involvement of chromosome 10 in translocations induced in vivo by low levels of ionizing radiation.

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supporting

confidence: 74%

FISH analysis of translocations in lymphocytes of children exposed to the Chernobyl fallout: preferential involvement of chromosome 10

Scarpato

Lori

Panasiuk

et al. 1997

Cytogenet Genome Res

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“…Many authors report increased genetic damage in peripheral blood lymphocytes of human populations exposed to relatively low-dose occupational radiation exposure. For example, significant increases in the incidence of dicentrics plus rings were observed in follow up studies of populations around Chernobyl [24–28], liquidators of the Chernobyl accident [27], and the population living in buildings with chronic low-dose exposure [29]. Significant association between translocation frequency and accumulated dose in nuclear workers was observed by many authors [30, 31].…”

Section: Discussionmentioning

confidence: 99%

Chromosome analysis of nuclear power plant workers using fluorescence in situ hybridization and Giemsa assay

Hristova¹,

Hadjidekova²,

Grigorova³

et al. 2013

Journal of Radiation Research

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The aim of this study was to evaluate the genotoxic effects of ionizing radiation in vivo in exposed Bulgarian nuclear power plant workers by using classical cytogenetic and molecular cytogenetic analyses of peripheral lymphocytes. Chromosome analysis using fluorescence in situ hybrydization (FISH) and Giemsa techniques was undertaken on 63 workers and 45 administrative staff controls from the Bulgarian Nuclear Power Plant. Using the Giemsa method, the frequencies of cells studied with chromosome aberrations, dicentrics plus rings and chromosome fragments in the radiation workers were significantly higher compared with the control group (P = 0.044, P = 0.014, and P = 0.033, respectively). A significant association between frequencies of dicentrics plus rings and accumulated doses was registered (P < 0.01). In the present study, a FISH cocktail of whole chromosome paints for chromosomes 1, 4 and 11 was used. A significant association between frequency of translocations and accumulated doses was also observed (P < 0.001). Within the control group, a correlation was found between age and the spontaneous frequency of translocations. No correlation was found between smoking status and frequency of translocations. When compared with the control group, workers with accumulated doses up to 100 mSv showed no increase in genome translocation frequency, whereas workers with accumulated doses from 101 to 200 mSv showed a statistically significant doubling of genome translocation frequency (P = 0.009). Thus, in cases of chronic exposure and for purposes of retrospective dosimetry, the genome frequency of translocations is a more useful marker for evaluation of genotoxic effects than dicentric frequency.

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“…This correct dose was computed as an average of the collective biological dosimetry, with weights based on variances of the measurements for each technique. In this regard, the present study works toward the goal of collective dosimetry for dose reconstruction (16,27,29,82,83). However, comparisons in the scientific literature between biological and physical methods of radiation dosimetry remain infrequent (80,(84)(85)(86).…”

Section: Discussionmentioning

confidence: 99%

Collective Radiation Biodosimetry for Dose Reconstruction of Acute Accidental Exposures: A Review

Pass

1997

Environmental Health Perspectives

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Quantification of the biologically relevant dose is required to establish cause and effect between radiation detriment or burden and important biological outcomes. Most epidemiologic studies of unanticipated radiation exposure fail to establish cause and effect because researchers have not been able to construct a valid quantification of dose for the exposed population. However, no one biodosimetric technique (biophysical or biological) meets all the requirements of an ideal dosimeter. This paper reviews how the collection of biodosimetric data for victims of radiation accidents can be used to create a dosimetric "gold standard." Particular emphasis is placed on the use of electron spin resonance, a standard for radiation accident dosimetry. As an example of this technique, a review will be presented of a previously reported study of an individual exposed to a 60Co sterilization source.

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Dicentric and translocation analysis for retrospective dose estimation in humans exposed to ionising radiation during the Chernobyl nuclear power plant accident

Cited by 74 publications

References 10 publications

FISH analysis of translocations in lymphocytes of children exposed to the Chernobyl fallout: preferential involvement of chromosome 10

FISH analysis of translocations in lymphocytes of children exposed to the Chernobyl fallout: preferential involvement of chromosome 10

Chromosome analysis of nuclear power plant workers using fluorescence in situ hybridization and Giemsa assay

Collective Radiation Biodosimetry for Dose Reconstruction of Acute Accidental Exposures: A Review

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