Diceratocephala boschmai (Platyhelminthes: Temnocephalida) from crayfish farms in Thailand: investigation of the topographic surface and analysis of 18S ribosomal DNA sequences

Contributions to the knowledge of Pseudolevinseniella (Trematoda: Digenea) and temnocephalans from alien crayfish in natural freshwaters of Thailand

“…In the Diceratocephalidae studied here, the organ positions were similar to those described by Ngamniyon et al. (2014) and were maintained in C. destructor from local farming of freshwater crayfish in Thailand.…”

“…Sample sequences were identified using the Basic Local Alignment Search Tool (BLAST) (Mount, 2007) for the local similarity of 18S, 28S rDNA and cox1 from the nucleotide databases of The National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov). For other flatworms and outgroup species, nucleotide sequences were searched from these databases (accession numbers from Cribb et al., 2001; Lockyer et al., 2003; Van Steenkiste et al., 2013; Fraija-Fernandez et al., 2015; Vdacny et al., 2011; Laumer and Giribet, 2014; Ngamniyon et al., 2014; Tkach et al., 2003; Devi et al., 2010; Vdacny et al., 2012; Hoyal-Cuthill et al., 2016; Leung et al., 2009; Keeney et al., 2009; Ng et al., 2018). Sequences were aligned with the Clustal Omega multiple sequence alignment programme (http://www.genome.jp/tools/clustalw/), and phylogenetic trees inferred using the maximum-likelihood approach in MEGA 5.1 (Tamura et al., 2011).…”

“…Volonterio (2009) described D. boschmai Baer (1953) collected from C. quadricarinatus on a Uruguayan crayfish farm. Ngamniyon et al. (2014) found D. boschmai on the bodies of C. destructor from local farms of freshwater crayfish in Thailand.…”

Molecular characterization of two Australian temnocephalans (Temnocephalida, Platyhelminthes) introduced with alien crayfish (Parastacidae, Decapoda) into South Africa

“…quadricarinatus (Genbank accession MW443039) at only 2 sites of the 528 that could be compared). The host of the worm from which this Thai sequence came was Cherax destructor Clark, 1936 introduced from Australia (Ngamniyon et al, 2014). We did not include this sequence in our analysis because of its shorter length.…”

“…Temnocephalans (Platyhelminthes, Temnocephalida) are ectocommensal worms, which occur mainly on freshwater crustacean hosts, particularly crayfish of the family Parastacidae (Cannon, 1991) (Williams, 1981). Temnocephalans have a wide natural distribution throughout the Neotropical, Australian and Oriental realms (Chiesa et al, 2015; Damborenea & Brusa, 2009; Martínez‐Aquino et al, 2014; Ngamniyon et al, 2014; Ohtaka et al, 2012; Oki et al, 1995; Sewell, 2013; Sluys et al, 2010; Snovsky & Galil, 2011; Volonterio, 2009). They have a direct life cycle, laying eggs on the host from which juvenile worms emerge (Joffe, 1981).…”