Didymella corylicola sp. nov., a new fungus associated with hazelnut fruit development in Italy

Scarpari, Marzia; Vitale, S.; Giambattista, G. Di; Luongo, L.; Gregorio, Tommaso De; Schreiber, Giulio; Petrucci, Mariangela; Belisario, A.; Voglmayr, Hermann

doi:10.1007/s11557-020-01562-y

Cited by 15 publications

(10 citation statements)

References 24 publications

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“…In previous studies, Didymellaceae species were identified mainly according to their morphologies and plant–host relationships, which are limited in species determination [ 24 , 45 ]. In recent years, phylogenetic analysis based on multi-loci has been developed for taxon analysis in Phoma and phoma-like genera [ 45 , 46 ], and the multi-loci (ITS, LSU, rpb2 , and tub2 ) provide a solid phylogenetic pillar for the determination of Didymellaceae taxa [ 22 , 47 , 48 , 49 , 50 ]. Here, we utilized these multi-loci for phylogenetic analysis, revealing that these obtained isolates associated with LBSD symptoms of tea plants belong to two novel species, tentatively named D. theae and D. theifolia to refer to their host plants, with the suffix “– folia ” used for a distinction from the former.…”

Section: Discussionmentioning

confidence: 99%

Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease

Song³

et al. 2022

JoF

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Amplicon sequencing is a powerful tool for analyzing the fungal composition inside plants, whereas its application for the identification of etiology for plant diseases remains undetermined. Here, we utilize this strategy to clarify the etiology responsible for tea leaf brown-black spot disease (LBSD), a noticeable disease infecting tea plants etiology that remains controversial. Based on the ITS-based amplicon sequencing analysis, Didymella species were identified as separate from Pestalotiopsis spp. and Cercospora sp., which are concluded as the etiological agents. This was further confirmed by the fungal isolation and their specific pathogenicity on diverse tea varieties. Based on the morphologies and phylogenetic analysis constructed with multi-loci (ITS, LSU, tub2, and rpb2), two novel Didymella species—tentatively named D. theae and D. theifolia as reference to their host plants—were proposed and characterized. Here, we present an integrated approach of ITS-based amplicon sequencing in combination with fungal isolation and fulfillment of Koch’s postulates for etiological identification of tea plant disease, revealing new etiology for LBSD. This contributes useful information for further etiological identification of plant disease based on amplicon sequencing, as well as understanding, prevention, and management of this economically important disease.

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Section: Discussionmentioning

confidence: 99%

Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease

Song³

et al. 2022

JoF

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“…Using our results from UNITE, NCBI, and T-BAS ( supplementary fig. 4 ) to guide taxon sampling, we searched the literature for existing phylogenies and available genetic marker sequences for the different lineages to which our samples potentially belonged ( Nygren 2011 ; Wang 2016 , 2022 ; Chen et al 2017 ; Wanasinghe 2017 ; Crous 2019 , 2021 ; Jaklitsch 2018 ; Valenzuela-Lopez 2018 ; Hyde 2019 ; Hou 2020 ; Scarpari 2020 ; Vieira 2020 ; Jiang 2021 ; Karácsony et al 2021 ; Liu 2022 ; Wanasinghe and Mortimer 2022 ). Various combinations of 13 genetic markers were selected for the different lineages, sequences for which were retrieved from GenBank ( supplementary table 4 ).…”

Section: Methodsmentioning

confidence: 99%

Tapping Culture Collections for Fungal Endophytes: First Genome Assemblies for Three Genera and Five Species in theAscomycota

Hill

Levicky

Pitsillides

et al. 2023

Genome Biology and Evolution

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The Ascomycota form the largest phylum in the fungal kingdom and show a wide diversity of lifestyles, some involving associations with plants. Genomic data is available for many ascomycetes that are pathogenic to plants, but endophytes, which are asymptomatic inhabitants of plants, are relatively understudied. Here, using short- and long-read technologies, we have sequenced and assembled genomes for 15 endophytic ascomycete strains from CABI’s culture collections. We used phylogenetic analysis to refine the classification of taxa, which revealed that 7 of our 15 genome assemblies are the first for the genus and/or species. We also demonstrated that cytometric genome size estimates can act as a valuable metric for assessing assembly ‘completeness’, which can easily be overestimated when using BUSCOs alone and has broader implications for genome assembly initiatives. In producing these new genome resources, we emphasise the value of mining existing culture collections to produce data that can help to address major research questions relating to plant–fungal interactions.

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“…The phylogenetic tree was constructed with four loci, ITS, LSU, rpb2 and tub2, to identify these isolates of Didymellaceae at the species level. The reference strains were selected on the basis of the high sequence similarity of BLAST searches of ITS in GenBank and the adjacent strains provided by recent studies of Didymellaceae [54][55][56][57][58][59]. We selected 310 isolates that had been sequenced and deposited in GenBank as reference strains, referring to 34 representative genera.…”

Section: Sequence Alignment and Phylogenetic Analysismentioning

confidence: 99%

New Species of Didymellaceae within Aquatic Plants from Southwestern China

Chen

Wang

Jiang

et al. 2023

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Members of Didymellaceae have a wide geographical distribution throughout different ecosystems, and most species are associated with fruit, leaf, stem and root diseases of land plants. However, species that occur in aquatic plants are not clearly known. During a survey of the diversity of endophytes in aquatic plants in Yunnan, Sichuan, and Guizhou provinces, we obtained 51 isolates belonging to Didymellaceae based on internal transcribed spacer region (ITS) sequences. Further, the phylogenetic positions of these isolates were determined by combined sequences composed of ITS, partial large subunit nrRNA gene (28S nrDNA; LSU), RNA polymerase II second largest subunit (rpb2) and partial beta-tubulin gene (tub2). Combining morphological characteristics and multi-locus phylogenetic analyses, two new varieties belong to Boeremia and 12 new species distributed into seven genera were recognized from 51 isolates, i.e., Cumuliphoma, Didymella, Dimorphoma, Ectophoma, Leptosphaerulina, Remotididymella, and Stagonosporopsis. Among these species, only one species of Stagonosporopsis and two species of Leptosphaerulina show teleomorphic stages on OA, but have no anamorphic state. Each new species is described in detail, and the differences between new species and their phylogenetically related species are discussed here. The high frequency of new species indicates that aquatic plants may be a special ecological niche which highly promotes species differentiation. At the same time, the frequent occurrence of new species may indicate the need for extensive investigation of fungal resources in those aquatic environments where fungal diversity may be underestimated.

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Didymella corylicola sp. nov., a new fungus associated with hazelnut fruit development in Italy

Cited by 15 publications

References 24 publications

Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease

Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease

Tapping Culture Collections for Fungal Endophytes: First Genome Assemblies for Three Genera and Five Species in theAscomycota

New Species of Didymellaceae within Aquatic Plants from Southwestern China

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