Die Bestimmung der p-Aminobenzoesäure mit Fluram
 ®
 im Urin nach Durchführung des Pankreasfunktionstests mit Bentiromid

Eisenwiener, Hans-Georg; Morger, F; Lergier, W.; Gillessen, Dieter

doi:10.1515/cclm.1982.20.8.557

Cited by 3 publications

(3 citation statements)

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“…Para ‐aminobenzoic acid in urine . We determined para ‐aminobenzoic acid spectrophotometrically with fluorescamine (Roche, 071088) as described previously [19]. Addition of 200 μg of para ‐aminobenzoic acid to 1 mL of urine yielded a recovery of 96% ( n = 2).…”

Section: Methodsmentioning

confidence: 99%

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Roos

Meyboom

Kosmeijer-Schuil

et al. 1998

Journal of Internal Medicine

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Abstract. de Roos B, Meyboom S, Kosmeijer-Schuil TG, Katan MB (Wageningen Agricultural University, Wageningen, The Netherlands). Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers. J Intern Med 1998; 244: 451-60.Objectives. To determine the absorption and urinary excretion of the cholesterol-raising coffee diterpenes cafestol and kahweol in humans. Subjects and design. Nine healthy ileostomists consumed a dose of one, two or three cups of Frenchpress coffee together with a standardized breakfast on three separate days in random order. Subsequently, ileostomy effluent was collected for 14 h and urine for 24 h. Stability of cafestol and kahweol was also assessed under simulated gastrointestinal tract conditions. Main outcome measures. Absorption of diterpenes, stability of diterpenes during incubation with gastrointestinal fluids, and urinary excretion of diterpenes. Results. Corrected mean absorptions expressed as percentages of the amount consumed and the amount entering the duodenum were 67 and 88%, respectively, for cafestol, and 72 and 93%, respectively, for kahweol. We found losses of diterpenes during incubation in vitro with gastric juice (cafestol, 24%; kahweol, 32%), during storage with ileostomy effluent (cafestol, 18%; kahweol, 12%), and during freeze-drying (cafestol, 26%; kahweol, 32%). Mean excretion of glucuronidated plus sulphated conjugates in urine was 1.2% of the ingested amount for cafestol and 0.4% of the ingested amount for kahweol. Conclusions. About 70% of the ingested cafestol and kahweol is absorbed in ileostomy volunteers. Possibly, undetected metabolites are present in ileostomy effluent, resulting in lower absorption percentages. Only a small part of the diterpenes is excreted as a conjugate of glucuronic acid or sulphate in urine. Therefore, these compounds are extensively metabolized in the human body.

show abstract

Section: Methodsmentioning

confidence: 99%

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Roos

Meyboom

Kosmeijer-Schuil

et al. 1998

Journal of Internal Medicine

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show abstract

“…Para-aminobenzoic acid in urine. We determined paraaminobenzoic acid spectrophotometrically with fluorescamine (Roche, 071088) as described previously [19]. Addition of 200 g of para-aminobenzoic acid to 1 mL of urine yielded a recovery of 96% (n ϭ 2).…”

Section: Cafestol and Kahweol In Coffee And Gastrointestinal Fluidsmentioning

confidence: 99%

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

et al. 1998

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de Roos B, Meyboom S, Kosmeijer-Schuil TG, Katan MB (Wageningen). Absorption and uri-nary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers. J Intern Med 1998; 244: 451-60. Objectives. To determine the absorption and urinary excretion of the cholesterol-raising coffee diterpenes cafestol and kahweol in humans. Subjects and design. Nine healthy ileostomists consumed a dose of one, two or three cups of French-press coffee together with a standardized breakfast on three separate days in random order. Subsequently, ileostomy effluent was collected for 14 h and urine for 24 h. Stability of cafestol and kahweol was also assessed under simulated gastro-intestinal tract conditions. Main outcome measures. Absorption of diterpenes, stability of diterpenes during incubation with gastrointestinal fluids, and urinary excretion of diterpenes. Results. Corrected mean absorptions expressed as percentages of the amount consumed and the amount entering the duodenum were 67 and 88%, respectively, for cafestol, and 72 and 93%, respectively , for kahweol. We found losses of diterpenes during incubation in vitro with gastric juice (cafestol, 24%; kahweol, 32%), during storage with ileostomy effluent (cafestol, 18%; kahweol, 12%), and during freeze-drying (cafestol, 26%; kahweol, 32%). Mean excretion of glucuronidated plus sulphated conjugates in urine was 1.2% of the ingested amount for cafestol and 0.4% of the ingested amount for kahweol. Conclusions. About 70% of the ingested cafestol and kahweol is absorbed in ileostomy volunteers. Possibly, undetected metabolites are present in ileostomy effluent , resulting in lower absorption percentages. Only a small part of the diterpenes is excreted as a conjugate of glucuronic acid or sulphate in urine. Therefore, these compounds are extensively metabolized in the human body.

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“…This obviated the necessity of prior toxicologic studies. (4) In our HPLC assay for PABA,' PAS was detected also with a retention time suitable for accurate quantification of both compounds. As reported here for five healthy volunteers day to day variations in PAS excretion were not always negligible, confirming the day to day variations in PABA excretion reported by Kay et al 2 As a consequence, in one case a difference of 0-22 was found between PABA/PAS ratio and PABA excretion index (Table), although in the other case both parameters corresponded very well.…”

Section: Discussionmentioning

confidence: 97%

Improved specificity of the PABA test with p-aminosalicylic acid (PAS).

Hoek¹,

Bergh²,

Elhorst³

et al. 1987

Gut

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Die Bestimmung der p-Aminobenzoesäure mit Fluram^® im Urin nach Durchführung des Pankreasfunktionstests mit Bentiromid

Cited by 3 publications

References 0 publications

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Improved specificity of the PABA test with p-aminosalicylic acid (PAS).

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Die Bestimmung der p-Aminobenzoesäure mit Fluram ® im Urin nach Durchführung des Pankreasfunktionstests mit Bentiromid

Cited by 3 publications

References 0 publications

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Absorption and urinary excretion of the coffee diterpenes cafestol and kahweol in healthy ileostomy volunteers

Improved specificity of the PABA test with p-aminosalicylic acid (PAS).

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Die Bestimmung der p-Aminobenzoesäure mit Fluram^® im Urin nach Durchführung des Pankreasfunktionstests mit Bentiromid