Die Bildung der Oocystenh�lle bei Eimeria perforans (Sporozoa)

Scholtyseck, Erich; Voigt, W. H.

doi:10.1007/bf00339281

Cited by 38 publications

(10 citation statements)

References 9 publications

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“…The appearance of this antigen coincided with the presence of macrogamonts in the infected ceca and the development of WF II within the macrogamonts. The same 51-kDa antigen was also observed at 138 h p.i., a time when mature WF II were observed by transmission electron The inner wall of the oocyst is formed by material derived from WF II Scholtyseck and Voigt 1964). In the current study, the involvement of WF II antigens in oocyst formation was studied with the help of mAbs.…”

Section: Characterization Of the Antigens Recognized By Mab E2e5mentioning

confidence: 99%

“…The outer layer of the oocyst wall in Eimeria spp is formed by electron-dense WF I, which appears later in the development of macrogamont (Scholtyseck and Voigt 1964).…”

Section: Characterization Of the Antigens Recognized By Mab E1d8mentioning

confidence: 99%

“…Macrogametes of Eimeria spp can be ultrastructurally identified by two types of wall-forming bodies (WF I, WF II), involved in the building of the oocyst wall (Scholtyseck and Voigt 1964;Scholtyseck 1973). Electron microscopic studies of cell wall formation during macrogametogenesis in E. mivati (Wheat et al 1976), Eimeria spp (Wang 1982), Sarcocystis spp (Vertterling et al 1973) and Toxoplasma gondii (Ferguson et al 1975) have provided evidence for the role of WF I and WF II in oocyst wall genesis.…”

Section: Introductionmentioning

confidence: 99%

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Monoclonal antibodies specific for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa)

Mouafo

Weck-Heimann

Dubremetz

et al. 2002

Parasitology Research

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Two monoclonal antibodies (mAbs) raised against the macrogamonts of Eimeria tenella identified antigens located in the wall-forming bodies of type I (WF I) and type II (WF II) by indirect immunofluorescence and by immunoelectron microscopy. With these mAbs, the involvement of both types of wall-forming body at the protein level in the formation of the inner and outer oocyst walls of E. tenella was shown by indirect immunofluorescence assay. On Western blots of pure macrogamont, mAb E1D8 against WF I reacted with a series of bands between 42 kDa and 105 kDa. In pure, unsporulated extract, this mAb recognized a complex of bands between 26 kDa and 153 kDa. mAb E2E5 against WF II, on Western blots of pure extract of macrogamonts, recognized an antigen of 51 kDa. Later in the development, after the formation of the inner oocyst wall, mAb E2E5 reacted with three polypeptide of 23, 25 and 30 kDa. Proteolytic processing may be forwarded as the mechanism regulating the distinct regulation protein involved in the oocyst wall.

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Section: Characterization Of the Antigens Recognized By Mab E2e5mentioning

confidence: 99%

“…The outer layer of the oocyst wall in Eimeria spp is formed by electron-dense WF I, which appears later in the development of macrogamont (Scholtyseck and Voigt 1964).…”

Section: Characterization Of the Antigens Recognized By Mab E1d8mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monoclonal antibodies specific for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa)

Mouafo

Weck-Heimann

Dubremetz

et al. 2002

Parasitology Research

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“…Scholtyseck and Voigt (19) found, in an electron microscope investigation of E. perforam, that 2 different kinds of wall-forming bodies, as well as so-called "dark bodies", occurred in the cytoplasm of macrogametes. The wallforming bodies were found to form the outer and inner layers of the oocyst wall, whereas the function of the dark bodies was uncertain.…”

Section: From Henry (9)mentioning

confidence: 99%

Observations on the Life Cycle of Eimeria bilamellata Henry, 1932 in the Uinta Ground Squirrel *Spermophilus armatus**

Todd¹,

Hammond²,

Anderson³

1968

The Journal of Protozoology

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SYNOPSIS. Oocysts of Eimeria bilamellata were found in Spermophilus armatus, Utah and Wyoming; S. beecheyi, California; and S variegatus, Utah. Oocysts were not found in S. lateralis, S. richardsoni, S. columbianus, S. tridecemlineatus, or the white‐tailed prairie dog, Cynomys leucurus. Experimental infections were established in S. armatus, S. variegatus, and S. lateralis, but not in S. richardsoni, least chipmunks Eutamius minimus, laboratory rats Rattus norvegicus, or Mongolian gerbils Meriones unguiculatus. After one experimental infection S. armatus and S. variegatus were immune to further infections. Spermophilus lateralis could be infected 3 or 4 times before the animals were immune. However, individuals of S. armatus in a natural population had more than one infection with E. bilamellata; probably infections must be of a certain level before immunity develops. When S. armatus were inoculated with about 100,000 oocysts, the animals usually died on the 7th day after incoculation. Oocysts were 33‐37 by 25‐31 μ (mean 34.5 by 28.2 μ). The oocyst wall was brown and composed of 2 layers. A distinct micropyle was present. Sporocysts were 18‐23 by 9‐12 μ (mean 19.9 by 10.3 μ). In experimental infections, the prepatent period was 10 days and the patent period 5–21 (mean 9) days. Schizonts were 1st seen 7 days after inoculation. They were located above the host cell nuclei of epithelial cells at the tips of the villi of the jejunum and ileum. One or more earlier generations of schizonts were thought to occur, but these were not observed. Gametogony took place in epithelial cells of the jejunum and ileum. Shortly after the merozoites entered the cells, the cells became enlarged and were displaced into the lamina propria. The microgametocytes were considerably larger than the macrogametes and contained a central residual body. Macrogametes had a peripheral eosinophilic layer as well as cytoplasmic granules; both apparently participated in formation of the oocyst wall.

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“…Coccidian oocysts are crucial for the survival of the parasites in the external environment and the transmission to suitable hosts (Kheysin 1972 ). The oocyst wall, which is formed from proteins synthesized during the macrogametocyte development, has unique characteristics that protect the enclosed sporozoites from chemical and physical damage (Kheysin 1972 ; Scholtyseck and Voigt 1964 ). Hence, oocysts are resistant to disinfectants and chemicals, like sulfuric acid or potassium dichromate (Dubey et al 1970 ; Kheysin 1972 ; Marquardt 1966 ), although they are sensitive to heat, cold, and desiccation (Dubey 1998 ; Kheysin 1972 ; Ryley 1973 ).…”

Section: Introductionmentioning

confidence: 99%

Correlative light and electron microscopy of wall formation in Eimeria nieschulzi

et al. 2020

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Coccidian parasites possess complex life cycles involving asexual proliferation followed by sexual development leading to the production of oocysts. Coccidian oocysts are persistent stages which are secreted by the feces and transmitted from host to host guaranteeing life cycle progression and disease transmission. The robust bilayered oocyst wall is formed from the contents of two organelles, the wall-forming bodies type I and II (WFBI, WFBII), located exclusively in the macrogametocyte. Eimeria nieschulzi has been used as a model parasite to study and follow gametocyte and oocyst development. In this study, the gametocyte and oocyst wall formation of E. nieschulzi was analyzed by electron microscopy and immuno-histology. A monoclonal antibody raised against the macrogametocytes of E. nieschulzi identified a tyrosine-rich glycoprotein (EnGAM82) located in WFBII. Correlative light and electron microscopy was used to examine the vesicle-specific localization and spatial distribution of GAM82-proteins during macrogametocyte maturation by this monoclonal antibody. In early and mid-stages, the GAM82-protein is ubiquitously distributed in WFBII. Few hours later, the protein is arranged in subvesicular structures. It was possible to show that the substructure of WFBII and the spatial distribution of GAM82-proteins probably represent pre-synthesized cross-linked materials prior to the inner oocyst wall formation. Dityrosine-cross-linked gametocyte proteins can also be confirmed and visualized by fluorescence microscopy (UV light, autofluorescence of WFBII). Electronic supplementary material The online version of this article (10.1007/s00436-020-06765-6) contains supplementary material, which is available to authorized users.

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Die Bildung der Oocystenh�lle bei Eimeria perforans (Sporozoa)

Cited by 38 publications

References 9 publications

Monoclonal antibodies specific for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa)

Monoclonal antibodies specific for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa)

Observations on the Life Cycle of Eimeria bilamellata Henry, 1932 in the Uinta Ground Squirrel *Spermophilus armatus**

Correlative light and electron microscopy of wall formation in Eimeria nieschulzi

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Die Bildung der Oocystenh�lle bei Eimeria perforans (Sporozoa)

Cited by 38 publications

References 9 publications

Monoclonal antibodies specific for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa)

Monoclonal antibodies specific for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa)

Observations on the Life Cycle of Eimeria bilamellata Henry, 1932 in the Uinta Ground Squirrel Spermophilus armatus*

Correlative light and electron microscopy of wall formation in Eimeria nieschulzi

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Observations on the Life Cycle of Eimeria bilamellata Henry, 1932 in the Uinta Ground Squirrel *Spermophilus armatus**