Die Dünnschichtchromatographie in der Arzneimittelanalyse

Thin-Layer Chromatography. Recent Developments in Equipment and Applications

“…The plates must be thoroughly cleaned, preferably with sulfuric-chromic acid, followed by water, and dried. A slurry is prepared by mixing 15 grams of Silica Gel G with 50 ml. of water and shaking for about IV* minutes, and is used promptly.…”

Separation of water-soluble vitamins on starch thin layers

“…The flask and sample holder were rinsed with 0.017V sodium hydroxide to remove any absorbed fluoride. Analysis of the absorber solution for fluoride ion was accomplished via one of the various published colorimetric techniques (2).…”

Detection and determination by ultraviolet reflectance spectrometry of vitamins resolved on thin layer plates

“…tion. This approach was originally developed by Brockman and Volpers (5) and by Sease (6,7) for the location of colorless, ultraviolet absorbing material in column chromatography, and has subsequently been used by other investigators in conjunction with thin layer chromatography (8)(9)(10). The other method employed direct scanning of silica gel G chromatoplates by means of a spectrophotometer fitted with a standard reflectance attachment and set at a wavelength corresponding to the absorption maximum of the vitamin of interest.…”