Thin-Layer Chromatography. Recent Developments in Equipment and Applications

Insecticide Synergists, Identification of 3,4-Methylenedioxyphenyl Synergists by Thin-Layer Chromatography.

“…Instruments, Inc., Great Neck, N. Y. was used. A similar apparatus is available from Microchemical Specialties Co., Berkeley 3, Calif. Wolfish et al (23) Cool to room temperature and store in a glassstoppered container.…”

Incorporation of Tritium from Thymidine‐methyl‐H3 into DNA, RNA, Lipids and Protein in Logarithmic and Stationary Phase Tetrahymena pyriformis, Strain W*

“…as the adsorbent surface. Plates were prepared as outlined in Wollish, S c h m d and Hawrylshyn (29). Butano1:glacial acetic acid:walter ( 3 : l : l ) was the first solvent, phenol (Mallinckrodt):water (3:l) the second (8).…”

Application and Theory of Unidimensional Multiple Chromatography.

“…The R values were found to vary with the condition of drying, the thickness of the support layers, and length of exposure to the atmosphere. The lack of reproducibility may be in part the result of variations in the structure of the gel when treated in different ways (88, 83), To ensure uniform layering, the apparatus described by Wollish et al (44) is recommended.…”

“…The superb resolving power of UMC is achieved by effectively increasing the length of the chromatographic support and the number of theoretical plates over which the solutes migrate. Although UMC is capable of excellent resolution of simple mixtures (10,19), the excessive time required for multiple irrigations has probably curtailed its use. In recent years, however, centrifugally accelerated chromatography (5,18,26,27) and thin-layer chromatography, TLC [for recent reviews, see (33,44) ], have been introduced as analytical tools and both of these innovations have the advantage of decreasing solvent development time. For this reason, it is now feasible to employ a larger number of solvent passes when attempting to resolve simple mixtures.…”