Die Feinstruktur der Gallenblase und der Galleng�nge

The human gallbladder

“…Previous investigations on the human gallbladder have demonstrated positive PAS and Alcian blue (pH 1.2) reactions in the Golgi area of the principal cells, in their apical granules, and in the brush border [11]. The PA-TCH-silver proteinate method [12] for localization of neutral mucosubstances and the colloidal iron method [13] for localization of acid mucosubstances at the electron microscopical level have confirmed the light microscope findings.…”

“…The PA-TCH-silver proteinate method [12] for localization of neutral mucosubstances and the colloidal iron method [13] for localization of acid mucosubstances at the electron microscopical level have confirmed the light microscope findings. More exactly, the reactions were positive 1) in the innermost sacculi of the Golgi complex, 2) in the secretory granules of the Golgi area and of the apical zone, and 3) in the surface coat and on and between the microvilli [11]. These results demonstrate that the principal cells of the human gallbladder epithelium can synthesize, store, and secrete neutral and acid mucosubstances in the same granule.…”

“…Abundant dense bodies with a heterogeneous aspect characterize not only the principal cells of the human gallbladder, but also that of other mammals. In the mouse, for instance, their number increases with age; their heterogeneity is also visible in freeze-fracture replicas [15]. Some of the dense bodies show a positive acid-phosphatase reaction and therefore they are Iysosomes [15].…”

“…The acid mucosubstances are, at least in part, sulfated. Secretion of the granule content into the gallbladder lumen occurs through a very active exocytosis, a process frequently seen in the principal cells [11]. Mucosubstances are also produced in cultures of epithelial cells of the human gallbladder [14].…”

Presence of brush cells in the mouse gallbladder

“…The alternating arrangement of actin filaments and microtubules is a regular feature conferring a stiff appearance to the apical cytoplasm of BC. This pattern is even evident in freeze-fracture replicas of unfixed specimens (Luciano et al, 1973). In thin sections however, the preservation of both structures is critical and depends to a great extent on the fixative solution used (Luciano et al, 1981).…”